Digluconate and Isopropyl Alcohol Biocide Formulation

Abstract

Effective surface disinfection is a fundamental infection control strategy within healthcare. This study assessed the antimicrobial efficacy of novel biocide formulations comprising 5% and 2% eucalyptus oil (EO) combined with 2% chlorhexidine digluconate (CHG) and 70% isopropyl alcohol (IPA) contained within a wipe. The efficacy of this novel antimicrobial formulation to remove and eliminate methicillin-resistant Staphylococcus aureus (MRSA), Escherichia coli and Candida albicans from steel surfaces was investigated. Adpression studies of pre-contaminated wipes were also utilised to assess their potential to induce cross-contamination between hard surfaces. Furthermore, the bactericidal nature of the EO-formulation was established in addition to time-kill. The EO-containing formulations demonstrated bactericidal antimicrobial efficacy against all microorganisms and did not induce surface cross-contamination. There was no significant difference (p < 0.05) between the 5% and 2% EO formulations in their ability to remove microorganisms from steel surfaces, however both significantly (p < 0.05) removed more than the control formulations. Microbial biofilms were eliminated within 10 min (p < 0.05) when exposed to the EO formulations. Our novel EO-formulation demonstrated rapid antimicrobial efficacy for potential disinfection and elimination of microbial biofilms from hard surfaces and may therefore be a useful adjunct to current infection control strategies currently employed within healthcare facilities.

MoWa: A Disinfectant for Hospital Surfaces Contaminated With Methicillin-Resistant Staphylococcus aureus (MRSA) and Other Nosocomial Pathogens

“…Antimicrobial activity against MRSA and E. coli has also been demonstrated, with a similar efficacy to that presented in the current study, for antimicrobial formulations containing chlorhexidine gluconate and/or isopropyl alcohol ( Campos et al., 2012 ; Hendry et al., 2012 ). Ethanol wipes, and a portable UV radiation device, were also effective to eradicate contamination by MRSA and other nosocomial pathogens whereas spraying a solution of 70% ethanol only reduced the density of MRSA ( Panousi et al., 2009 ; Umezawa et al., 2012 ).…”

Chlorhexidine Gel Use in the Oral District: A Systematic Review

“…No bacterial resistance has been shown following the use of chlorhexidine, nor for the realization of opportunistic infections or negative characteristics for the oral microbiota. [64,65]. After three months of use of chlorhexidine gluconate in oral rinsing and then his stop, the number of plaque bacteria returned to basal levels.…”

Green biocides, a promising technology: current and future applications to industry and industrial processes

“…The possibility of synthesizing these molecules suggests the opportunity to scale up their production to meet future needs and challenges in this field. The alcohol‐based biocides inhibit cell wall formation and DNA and RNA synthesis. Low concentrations of phenoxyethanol can cause proton translocation in bacteria ethanol, methanol, isopropanol, benzyl alcohol, phenylethyl alcohol, 2‐bromo‐2‐nitro‐1,3‐diol (Bronopol), phenoxyethanol (phenoxetol), chlorbutanol (chlorbutol) and 2,4‐dichlorobenzyl alcohol .…”