Diisoprenyl Cyclohexene-Type Meroterpenoids with Cytotoxic Activity from a Mangrove Endophytic Fungus Aspergillus sp. GXNU-Y85

Abstract

Five new diisoprenyl cyclohexene-type meroterpenoids, aspergienynes J–N (1–5), along with three known analogues (6–8), were obtained from the mangrove endophytic fungal strain Aspergillus sp. GXNU-Y85. The chemical structures, including their absolute configurations, were established via spectroscopic data and comparison of experimental and calculated ECD spectra. Cytotoxicity assay results indicated that compound 8 had strong cytotoxicity against HeLa cancer cells, and its IC50 value was 11.8 μM. In addition, flow cytometry analysis revealed that the cytotoxicity of 8 was due to the induction of G1 cell cycle arrest and apoptosis in HeLa cells.

New Meroterpenoids and α-Pyrone Derivatives Isolated from the Mangrove Endophytic Fungal Strain Aspergillus sp. GXNU-Y85

“…In the present study, three new compounds (1, 2, and 4), one new natural compound (3), and five previously reported isolates (5)(6)(7)(8)(9) were acquired from the fungal strain Aspergillus sp. GXNU-Y85 by various chromatographic techniques.…”

“…In a continuous endeavour to identify the bioactive constituents derived from mangrove endophytic fungi [5,6], we isolated two new meroterpenoids, aspergienynes O and P (1 and 2), one new natural compound, aspergienyne Q (3), and a new α-pyrone derivative termed 3-(4-methoxy-2-oxo-2H-pyran-6-yl)butanoic acid (4), together with five previously reported compounds (5)(6)(7)(8)(9), from the secondary metabolites of Aspergillus sp. GXNU-Y85 derived from the fruits of the mangrove plant Kandelia candel in this study.…”