Dimethoxypropane Induced Transesterification of Fats and Oils in Preparation of Methyl Esters for Gas Chromatographic Analysis.

Mason, Michael E.; Waller, George R.

doi:10.1021/ac60209a008

Cited by 216 publications

(89 citation statements)

References 9 publications

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“…Fatty acid methyl esters were prepared according to the Method of Waller et al 16 , and analyzed by gas chromatography Model 7890A, Agilent Technologies equipped with a flame ionization detector FID , and a capillary chromatographic column DB-WAX . The split ratio was 10:1.…”

Section: Fatty Acid Analysismentioning

confidence: 99%

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Effects of Ultrasonic Parameters on the Crystallization Behavior of Virgin Coconut Oil

Cao

Bai

et al. 2016

J. Oleo Sci.

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Section: Fatty Acid Analysismentioning

confidence: 99%

“…VCO 5-10 mg was conditioned at 60 for 10 min to destroy crystal memories and then rapidly cooled down at a rate of 10 /min to an isothermal holding temperature [13][14][15][16][17][18][19] , which was maintained for 30 min. Next, the sample was heated at a rate of 2 /min to define the melting endotherm.…”

Section: Isothermal Crystallization Analysismentioning

confidence: 99%

Effects of Ultrasonic Parameters on the Crystallization Behavior of Virgin Coconut Oil

Cao

Bai

et al. 2016

J. Oleo Sci.

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“…Fatty acids were transesterified with a modified version of the procedure described by Mason and Waller (1964). The lipid samples (1 mg) were resuspended in 3 mL methanolic HCl (3M; Supelco, 33050-U) and subsequently incubated at 60°C for 15 min in a stoppered vial sealed with nitrogen.…”

Section: Transmethylation Proceduresmentioning

confidence: 99%

A critical assessment of different transmethylation procedures commonly employed in the fatty acid analysis of aquatic organisms

Schlechtriem

Henderson

Tocher

2008

Limnology & Ocean Methods

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Several transmethylation procedures have been used for fatty acid analysis of aquatic organisms, although the suitability of the applied procedures has rarely been tested. The aim of this study was to demonstrate how different derivatization procedures can affect the result of fatty acid analysis. Different transmethylation procedures based on the acidic catalysts boron trifluoride, concentrated sulphuric acid, and anhydrous hydrochloric acid were applied to cold-pressed copepod oil and Atlantic salmon flesh lipids rich in wax esters and triacylglycerols, respectively. The results show that (1) the use of unsuitable catalysts and/or incubation conditions may influence the data obtained, which can lead to inaccurate conclusions about the presence of fatty acids in aquatic organisms/ecosystems, (2) different derivatization procedures based on the same catalyst can produce diverging results, and (3) the efficiency of a selected catalyst/procedure should be verified (e.g., by thin-layer chromatography) to ensure the complete transmethylation of fatty acids.

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“…The samples were sub jected to 1 ml Supelclcan® LC-NHjSPE columns using a Visiprep® solid-phase extraction vacuum manifold (Supclco Inc., Bcllcfonte, Pa., USA) to effect lipid class separation [8] employing eluants as directed by Kaluzny et al [9], The fatty acids composition of the phos pholipid fraction was measured as methyl esters [10] with gas-liquid chromatography (Shimadzu GC-14A; Shimadzu Europa GmbH, Duisburg, Germany) equipped with a polar capillary column (SGE BPX70. diameter 0.33 mm, length 25 m) and using helium as a carrier gas.…”

Section: Analytical Proceduresmentioning

confidence: 99%

Serum Lipids in Children with Xeroderma from an Inland District of Sri Lanka

Christiansen

Skåra²,

Sørensen³

et al. 1995

Ann Nutr Metab

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Children living on plantations in inland districts of the southeastern part of Sri Lanka frequently develop a skin condition on the legs described as mosaic skin or xeroderma. This condition is characterized by atrophic, dry, shining and scaly skin. The etiology is unknown. A food frequency survey indicated a low energy intake, a diet with a low fat content, and anthropometric data have shown a high prevalence of malnutrition within this group. The skin condition brought attention to a possible deficiency of essential nutrients, especially essential fatty acids. In order to investigate the possible association with a deficiency of essential fatty acids, blood samples were collected from both children having signs of xeroderma and controls. The total amount of phospholipids was low, but the fatty acid profile of this lipid class was similar to the controls. A vitamin A deficiency was indicated by low levels of its transport proteins. A multifactorial etiology where vitamin A deficiency may play a role is discussed.

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Dimethoxypropane Induced Transesterification of Fats and Oils in Preparation of Methyl Esters for Gas Chromatographic Analysis.

Cited by 216 publications

References 9 publications

Effects of Ultrasonic Parameters on the Crystallization Behavior of Virgin Coconut Oil

Effects of Ultrasonic Parameters on the Crystallization Behavior of Virgin Coconut Oil

A critical assessment of different transmethylation procedures commonly employed in the fatty acid analysis of aquatic organisms

Serum Lipids in Children with Xeroderma from an Inland District of Sri Lanka

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