Dimethyl Sulfoxide Enhancement of Phlebotomus Fever Virus Plaque Formation

Jm, McCown; We, Brandt; Wh, Bancroft; Pk, Russell

doi:10.4269/ajtmh.1979.28.733

Cited by 6 publications

(4 citation statements)

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“…The CER cell line has been recognized as the most efficient cell line currently available for the growth of CCHF virus (5, 7). Clerx et al (7) produced plaques in CER cells by using the overlay method of McCown et al (12) and incorporating dimethyl sulfoxide and DEAE-dextran, but in the present study we produced plaques by incorporating dimethyl sulfoxide alone. Although it is less sensitive than mouse inoculation for the titration and isolation of CCHF virus, the plaque assay is of equivalent sensitivity to the fluorescence focus assay and is more convenient to perform and easier to read than the latter test in virus titration and neutralization tests, particularly when large numbers of specimens are involved.…”

mentioning

confidence: 57%

Comparison of methods for isolation and titration of Crimean-Congo hemorrhagic fever virus

Shepherd¹,

Swanepoel²,

Leman³

et al. 1986

J Clin Microbiol

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The fluorescence focus assay and the plaque assay in CER cells were compared with mouse inoculation for the isolation and titration of Crimean-Congo hemorrhagic fever virus. The fluorescence focus assay and the plaque assay were of similar sensitivity, but both produced 10to 100-fold lower titers than did mouse inoculation. For specimens from 26 Crimean-Congo hemorrhagic fever patients in South Africa, virus was isolated from 20 by mouse inoculation and from only 11 by cell culturing. Although cell cultures were less sensitive for the isolation of virus from clinical specimens, they produced diagnostic results much more rapidly.

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mentioning

confidence: 57%

Comparison of methods for isolation and titration of Crimean-Congo hemorrhagic fever virus

Shepherd¹,

Swanepoel²,

Leman³

et al. 1986

J Clin Microbiol

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“…For SFSV, concentrations were determined by a plaque assay as described earlier (6,18 in sterile water). After 4 days, a second layer of agar mixture, containing 60 g/ml neutral red, was added.…”

Section: Methodsmentioning

confidence: 99%

Ex Vivo Stability of the Rodent-Borne Hantaan Virus in Comparison to That of Arthropod-Borne Members of the Bunyaviridae Family

Hardestam

Simon

Hedlund

et al. 2007

Appl Environ Microbiol

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The possible effect of virus adaptation to different transmission routes on virus stability in the environment is not well known. In this study we have compared the stabilities of three viruses within the Bunyaviridae family: the rodent-borne Hantavirus Hantaan virus (HTNV), the sand fly-borne Phlebovirus sandfly fever Sicilian virus (SFSV), and the tick-borne Nairovirus Crimean-Congo hemorrhagic fever virus (CCHFV). These viruses differ in their transmission routes: SFSV and CCHFV are vector borne, whereas HTNV is spread directly between its hosts, and to humans, via the environment. We studied whether these viruses differed regarding stability when kept outside of the host. Viral survival was analyzed at different time points upon exposure to different temperatures (4°C, 20°C, and 37°C) and drying at 20°C. We observed clearly different stabilities under wet conditions, particularly at 4°C, where infectious SFSV, HTNV, and CCHFV were detectable after 528, 96, and 15 days, respectively. All three viruses were equally sensitive to drying, as shown by drying on aluminum discs. Furthermore, HTNV and SFSV partially survived for 2 min in 30% ethanol, whereas CCHFV did not. Electron microscopy images of HTNV, SSFSV, and CCHFV stored at 37°C until infectivity was lost still showed the occurrence of virions, but with abnormal shapes and densities compared to those of the nonincubated samples. In conclusion, our study points out important differences in ex vivo stability among viruses within the Bunyaviridae family.

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“…SFN and TOS were determined by a plaque reduction neutralization test (PRNT) as described by Earley and colleagues [13] but modified according to McCown and colleagues [14] by adding Sandfly fever antibodies among Cypriots dextran, dimethyl sulfoxide (1 %) and heparin (20 units/ml) to the agar overlay. The second overlay containing neutral red was added on day 4 for SFS and TOS and on day 7 for SFN.…”

Section: Serological Testsmentioning

confidence: 99%

High prevalence rates of antibody to three sandfly fever viruses (Sicilian, Naples and Toscana) among Cypriots

1991

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SUMMARYNeutralizing antibodies to sandfly fever Naples, sandfly fever Sicilian and Toscana viruses were investigated among 479 sera collected from a normal human population in Cyprus. Antibody prevalence rates of 57%, 32% and 20% were found to Naples, Sicilian and Toscana viruses, respectively. The observed frequency of dual and triple infections was higher than would be expected with a random chance of infection. Antibody prevalence rates were similar for men and women for all three viruses tested, but one of two study sites had significantly higher antibody prevalence to Naples and Sicilian viruses than the other. Individuals with antibodies to both Naples and Toscana viruses had higher antibody levels to Naples virus than those with antibodies to Naples virus only. If the antibody prevalence rates found in this study reflect a history of clinical disease as described in the literature, sandfly fever poses a significant public health problem in Cyprus.

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Dimethyl Sulfoxide Enhancement of Phlebotomus Fever Virus Plaque Formation

Cited by 6 publications

References 0 publications

Comparison of methods for isolation and titration of Crimean-Congo hemorrhagic fever virus

Comparison of methods for isolation and titration of Crimean-Congo hemorrhagic fever virus

Ex Vivo Stability of the Rodent-Borne Hantaan Virus in Comparison to That of Arthropod-Borne Members of the Bunyaviridae Family

High prevalence rates of antibody to three sandfly fever viruses (Sicilian, Naples and Toscana) among Cypriots

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