Dimethyl sulfoxide induces oxidative stress in the yeastSaccharomyces cerevisiae

Abstract

Dimethyl sulfoxide (DMSO) is used as a cryoprotectant for the preservation of cells, including yeast, and as a solvent for chemical compounds. We report that DMSO induces oxidative stress in the yeast. Saccharomyces cerevisiae wt strain EG-103 and its mutants Δsod1, Δsod2, and Δsod1 Δsod2 were used. Yeast were subjected to the action of 1-14% DMSO for 1 h at 28 °C. DMSO induced a concentration-dependent inhibition of yeast growth, the effect being more pronounced for mutants devoid of SOD (especially Δsod1 Δsod2). Cell viability was compromised. DMSO-concentration-dependent activity loss of succinate dehydrogenase, a FeS enzyme sensitive to oxidative stress, was observed. DMSO enhanced formation of reactive oxygen species, estimated with dihydroethidine in a concentration-dependent manner, the effect being again more pronounced in mutants devoid of superoxide dismutases. The content of cellular glutathione was increased with increasing DMSO concentrations, which may represent a compensatory response. Membrane fluidity, estimated by fluorescence polarization of DPH, was decreased by DMSO. These results demonstrate that DMSO, although generally considered to be antioxidant, induces oxidative stress in yeast cells.

Wine Phenolic Compounds: Antimicrobial Properties against Yeasts, Lactic Acid and Acetic Acid Bacteria

“…This finding is in accordance with results of Sadowska-Bartosz et al [19] reporting an inhibiting concentration of 5-10% v/v DMSO for this species. S. bayanus HL 77 showed delayed growth at 5% v/v, but reached still high cell densities after 4 days.…”

“…EUCAST (European Committee on Antimicrobial Susceptibility Testing) and CLSI (Clinical and Laboratory Standard Institute) recommend 1% v/v DMSO in the medium in inhibition experiments to exclude any solvent effect. On the other hand, Sardowska-Bartozs et al [19] found that Saccharomycetes are only inhibited >8% v/v DMSO. We found impaired growth of S. bayanus HL77 at 5% v/v DMSO and decided to perform the inhibition experiments at 5% and 2.5% DMSO in the medium.…”

Use ofade1andade2mutations for development of a versatile red/white colour assay of amyloid-induced oxidative stress insaccharomyces cerevisiae

“…If the observed red to white switch was indeed due to increased oxidative cellular environment, chemical agents that induce oxidative stress would also be expected to cause the red to white colour phenotypic switch. Indeed, when we checked the effect of one such oxidizing agent, DMSO (7%)(Sadowska‐Bartosz et al ., ), a similar white colour phenotype resulted which was also reversible to red when the DMSO was removed from the growth media (Figure B).…”

Proteome targets of ubiquitin‐like samp1ylation are associated with sulfur metabolism and oxidative stress in Haloferax volcanii

“…If so, as the ratio of DMSO to oxygen rises, the observed sampylation of MsrA/B could serve to inhibit these enzymes and maximize the availability of DMSO to serve as a terminal electron acceptor for energy production. In analogy to yeast , DMSO may also induce oxidative stress in Hfx. volcanii .…”