Dimethyloxallyl glycine/nanosilicates-loaded osteogenic/angiogenic difunctional fibrous structure for functional periodontal tissue regeneration

Shang, Luqing; Liu, Ziqi; Ma, Baojin; Shao, Jinlong; Wang, Bing; Ma, Chenxi; Ge, Shaohua

doi:10.1016/j.bioactmat.2020.10.010

Cited by 44 publications

(72 citation statements)

References 61 publications

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“…Besides, VEGF was a critical target gene and protein, which could be induced by upregulated HIF‐1α to efficiently promote vascularization in the regenerated tissue. [ 33 ] After 3 days of culture, the VEGF concentration of supernatants in HUVECs was measured by ELISA kit (Figure 4D). The results showed that the detected VEGF concentrations of HUVECs stimulated by conditioned medium from GP, BM/GP, DM/GP, and DBM/GP scaffolds were 120.45 ± 4.26, 200.65 ± 4.50, 245.55 ± 4.93, and 285.55 ± 6.48 ng L −1 , respectively, which were higher than that of control group (85.94 ± 4.81 ng/L).…”

Section: Resultsmentioning

confidence: 99%

“…Previous studies have shown that Si ions were able to trigger the osteogenic differentiation of BMSCs, [ 36 ] and the silica‐contained scaffolds significantly enhanced the osteogenic differentiation. [ 33,37 ] In addition, BFP, an osteogenic peptide derived from the immature region of BMP‐7, were decorated on the substrates or incorporated into the materials to enhance the osteogenic effects. [ 20a,21,38 ] Benefited from these chemical cues that can promote the osteogenic differentiation of BMSCs, an enhancement on osteogenesis‐related genes expression stimulated by silica and BFP‐contained DBM/GP scaffold was demonstrated in our experimental investigation.…”

Section: Resultsmentioning

confidence: 99%

“…[ 17,40a ] Furthermore, a difunctional DMOG/nanosilicate‐poly (lactic‐co‐glycolic acid) (DMOG/nSi‐PLGA) membrane was developed and showed admirable effects on periodontal tissue regeneration through the synergistic effects of DMOG and Si ions on angiogenesis and osteogenesis. [ 33 ] In our study, DMOG and BFP were incorporated into the microchannel networks‐enriched nanofibrous hybrid scaffold using the nanocarrier of MSNs, in which sequential release profile of the two factors were realized. The DMOG and BFP co‐loaded nanofibrous scaffold showed excellent biocompatibility and enhanced osteogenic and angiogenic potential as well as promoted healing of skull bone defects.…”

Section: Resultsmentioning

confidence: 99%

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Bone Microenvironment‐Mimetic Scaffolds with Hierarchical Microstructure for Enhanced Vascularization and Bone Regeneration

et al. 2022

Adv Funct Materials

117

111

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Microchannel networks within engineered 3D scaffold can allow nutrient exchange and rapid blood vessels formation. However, fabrication of a bone microenvironment‐mimicking scaffold with hierarchical micro/nanofibrous and microchannel structures is still a challenge. Herein, inspired by structural and functional cues of bone remodeling, a microchannel networks‐enriched nanofibrous scaffold by using 3D printing and thermally induced phase separation techniques, which can facilitate cells migration and nutrients transportation, is developed. The customizable vascular‐like structure of polycaprolactone within the nanofibrous gelatin‐silica scaffold is fabricated using 3D‐printed sacrificial templates, while dimethyloxalylglycine (DMOG)‐loaded mesoporous silica nanoparticles (MSNs) located on the scaffold surface and bone forming peptide‐1 (BFP)‐loaded MSNs embedded in the scaffold are implemented for sequential release of DMOG and BFP. The cell experiments show that dual‐drug delivery scaffold (DBM/GP) promotes angiogenesis by stimulating migration, tube formation, and angiogenesis‐related genes/protein expression of endothelial cells, and osteogenesis by promoting osteo‐related genes expression and mineral deposition of osteoblasts. Additionally, DBM/GP scaffold facilitates the angiogenic activity of osteoblasts by activating phosphatidylinositol 3‐kinase/protein kinase B/hypoxia inducible factor‐1α pathway. Furthermore, enhanced vascularization and bone regeneration of DBM/GP scaffold are demonstrated via subcutaneous and skull defect models. Overall, this study reveals that the bone microenvironment‐mimetic dual‐drug delivery scaffold provides a promising strategy for bone defects treatment.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Bone Microenvironment‐Mimetic Scaffolds with Hierarchical Microstructure for Enhanced Vascularization and Bone Regeneration

et al. 2022

Adv Funct Materials

117

111

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“…In this context, the recent developments in the preparation of mesoporous bioactive glass nanoparticles could provide a very interesting alternative for this purpose [ 17 , 18 , 19 , 20 ]. On the other hand, the coupling of osteogenesis and angiogenesis is crucial in periodontal tissue regeneration and biomaterials loaded with different agents that act synergistically on both processes are very recently being designed to achieve periodontal regeneration [ 21 ].…”

Section: Introductionmentioning

confidence: 99%

Ipriflavone-Loaded Mesoporous Nanospheres with Potential Applications for Periodontal Treatment

et al. 2020

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The incorporation and effects of hollow mesoporous nanospheres in the system SiO2–CaO (nanoMBGs) containing ipriflavone (IP), a synthetic isoflavone that prevents osteoporosis, were evaluated. Due to their superior porosity and capability to host drugs, these nanoparticles are designed as a potential alternative to conventional bioactive glasses for the treatment of periodontal defects. To identify the endocytic mechanisms by which these nanospheres are incorporated within the MC3T3-E1 cells, five inhibitors (cytochalasin B, cytochalasin D, chlorpromazine, genistein and wortmannin) were used before the addition of these nanoparticles labeled with fluorescein isothiocyanate (FITC–nanoMBGs). The results indicate that nanoMBGs enter the pre-osteoblasts mainly through clathrin-dependent mechanisms and in a lower proportion by macropinocytosis. The present study evidences the active incorporation of nanoMBG–IPs by MC3T3-E1 osteoprogenitor cells that stimulate their differentiation into mature osteoblast phenotype with increased alkaline phosphatase activity. The final aim of this study is to demonstrate the biocompatibility and osteogenic behavior of IP-loaded bioactive nanoparticles to be used for periodontal augmentation purposes and to shed light on internalization mechanisms that determine the incorporation of these nanoparticles into the cells.

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“…However, studies have recognized that purely applying PDLSCs barely regenerates ideal periodontal tissue. Therefore, numerous studies have focused on facilitating the capacities of PDLSCs to improve the effect of periodontal regeneration (Shang et al, 2017(Shang et al, , 2021bAbdelaziz et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Epithelial Cell Rests of Malassez Provide a Favorable Microenvironment for Ameliorating the Impaired Osteogenic Potential of Human Periodontal Ligament Stem Cells

Liu

Zhang

et al. 2021

Front. Physiol.

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Human periodontal ligament stromal/stem cells (PDLSCs) are ideal candidates for periodontal regeneration and are of significant importance in clinical practice. However, PDLSCs derived from diseased microenvironments exert impaired behavior, which leads to the failure of periodontal regeneration. The epithelial cell rests of Malassez (ERM), which are involved in periodontal homeostasis, are residual cells from Hertwig's epithelial root sheath (HERS). However, the function of ERM remains largely unknown. Therefore, the aim of this study was to evaluate the effect of ERM on the osteogenic potential of PDLSCs from an impaired microenvironment. PDLSCs from healthy donors (H-PDLSCs), periodontitis donors (P-PDLSCs) and human ERM were harvested. Osteogenic evaluation showed a lower osteogenic potential of P-PDLSCs compared to that of H-PDLSCs. Then, we co-cultured ERM with P-PDLSCs, and the data showed that ERM promoted the expression of osteogenic genes and proteins in P-PDLSCs. In addition, we collected the PDLSCs from aged donors (A-PDLSCs) and analyzed the osteogenesis capacity of the A-PDLSCs and A-PDLSCs + ERM groups, which displayed similar results to P-PDLSCs. Finally, we evaluated the Wnt pathway, which is associated with osteogenic differentiation of stromal/stem cells, in A-PDLSCs + ERM and P-PDLSCs + ERM groups, which indicated that suppression of the Wnt pathway may result in an increase in the osteogenic properties of A-PDLSCs + ERM and P-PDLSCs + ERM groups. Taken together, the above findings shed new light on the function of ERM and provide a novel therapeutic for optimizing PDLSCs-based periodontal regeneration.

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Dimethyloxallyl glycine/nanosilicates-loaded osteogenic/angiogenic difunctional fibrous structure for functional periodontal tissue regeneration

Cited by 44 publications

References 61 publications

Bone Microenvironment‐Mimetic Scaffolds with Hierarchical Microstructure for Enhanced Vascularization and Bone Regeneration

Bone Microenvironment‐Mimetic Scaffolds with Hierarchical Microstructure for Enhanced Vascularization and Bone Regeneration

Ipriflavone-Loaded Mesoporous Nanospheres with Potential Applications for Periodontal Treatment

Epithelial Cell Rests of Malassez Provide a Favorable Microenvironment for Ameliorating the Impaired Osteogenic Potential of Human Periodontal Ligament Stem Cells

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