Diminished Urinary Free Cortisol Excretion in Patients with Moderate and Severe Renal Impairment

Abstract

periment might be a valuable alternative to the procedure described by Streit et al. (12 ) when analyte-free samples are not available.Our method is specific, sensitive, reproducible, and accurate (see Table 1 of the online Data Supplement). A 96-well plate format sample pretreatment in combination with LC-MS/MS for homocysteine analysis has been described previously (7,9 ). We consider the combination of this format with a large plasma dilution without deproteinization for high-throughput homocysteine analysis the most important aspect of our application.We thank Dr. Hartmut Kirchherr (Labor Bremen) for scientific discussions.

Defensive coping and renovascular disease risk — Adrenal fatigue in a cohort of Africans and Caucasians: The SABPA study

“…However, the cumulative effects of low cortisol will be evident in the cortisol diurnal pattern as the integrity of the HPAA is essential to an array of physiological systems [35]. Ultimately, dysfunctional HPAA activity and decreased cortisol release are associated with renal impairment [8,41], and our results confirmed this trend, but only in the Africans.…”

The degree of urinary hypercortisolism is not correlated with the severity of cushing’s syndrome

“…An important advantage of this method is its reliability [36], although it is crucially dependent on the quality and performance of the particular analytical procedure applied. Indeed, several conditions may result in falsely elevated values such as alcoholism, high fluid intake, pregnancy, psychiatric disorders, or in falsely low levels such as cyclic CS or severe renal function [37,38]. In this study, UFC levels were analyzed at three different laboratories, by different techniques, ECLIA and Immulite analytical procedures, even though the samples belonging to the same patients were measured in the same laboratory.…”

Screening Tests for Cushing's Syndrome: Urinary Free Cortisol Role Measured by LC-MS/MS

“…We found a high variability in multiple collections (as previously reported (8,16)), with similar CV in CS and non-CS. A limitation of this study is that we don't look at the creatinine to determine adequacy of the collection, although high urinary volumes cause an increase in UFC (17); furthermore, patients with CS may present with low urinary creatinine levels because of muscle wasting (16).…”