Dioclea violacea lectin ameliorates inflammation in the temporomandibular joint of rats by suppressing intercellular adhesion molecule-1 expression

Clemente-Napimoga, Juliana Trindade; Silva, Maria A. S. M.; Peres, Sylvia N. C.; Lopes, Alexandre H.; Lossio, Claudia Figueiredo; Oliveira, M. R. de; Osterne, Vinicius Jose Da Silva; Nascimento, Kyria S.; Abdalla, Henrique Ballassini; Teixeira, Juliana Maia; Cavada, Benildo Sousa; Napimoga, Marcelo Henrique

doi:10.1016/j.biochi.2018.12.007

Cited by 16 publications

(6 citation statements)

References 70 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Male Wistar rats (200–250 g) were briefly anesthetized by inhalation of 3% isoflurane (30 s) and pretreated with an intra-TMJ injection (20 μL) of NCL-NPX (0.6 mg). After 15 min, 4 h, 6 h, 12 h, 24 h, 3 d, 7 d or 10 d animals were treated with intra-TMJ injection (50 μL) of carrageenan (100 μg), a non-neurogenic inflammation agent on TMJ, leading to high levels of leukocytes migration 46 . After 1 hour (the peak for leukocyte migration in this inflammatory model), the animals were euthanized by deeply anesthesia.…”

Section: Methodsmentioning

confidence: 99%

Improved efficacy of naproxen-loaded NLC for temporomandibular joint administration

Guilherme

Ribeiro

Alcántara

et al. 2019

Sci Rep

Self Cite

View full text Add to dashboard Cite

Inflammatory conditions of the temporomandibular joint (TMJ) and peripheral tissues affect many people around the world and are commonly treated with non-steroidal anti-inflammatory drugs (NSAIDs). However, in order to get desirable results, treatments with NSAIDs may take weeks, causing undesirable side effects and requiring repeated administration. In this sense, this work describes the development of an optimized nanostructured lipid carrier (NLC) formulation for intra-articular administration of naproxen (NPX). An experimental design (2 3 ) selected the best formulation in terms of its physicochemical and structural properties, elucidated by different methods (DLS, NTA, TEM, DSC, and ATR-FTIR). The chosen formulation (NLC-NPX) was tested on acute inflammatory TMJ nociception, in a rat model. The optimized excipients composition provided higher NPX encapsulation efficiency (99.8%) and the nanoparticles were found stable during 1 year of storage at 25 °C. In vivo results demonstrated that the sustained delivery of NPX directly in the TMJ significantly reduced leukocytes migration and levels of pro-inflammatory cytokines (IL-1β and TNF-α), for more than a week. These results point out the NLC-NPX formulation as a promising candidate for the safe treatment of inflammatory pain conditions of TMJ or other joints.

show abstract

Section: Methodsmentioning

confidence: 99%

Improved efficacy of naproxen-loaded NLC for temporomandibular joint administration

Guilherme

Ribeiro

Alcántara

et al. 2019

Sci Rep

Self Cite

View full text Add to dashboard Cite

show abstract

“…Freitas et al have reported beneficial effects of Dvl in curing acute kidney injury due to its ability to reduce renal vascular resistance, apoptosis and ROS generation (Freitas et al, 2015). Another study has linked the anti-inflammatory effect of Dvl due to its ability of inhibiting plasma protein extravasation and leukocyte infiltration in rat model by decreasing ICAM-1 expression (Clemente-Napimoga et al, 2019). Another lectin isolated from Canavalia grandiflora (ConGF), a D-glucose/Dmannose-specific lectin is shown to inhibit the production of various pro-inflammatory cytokines like IL-1β and TNF-α thus affecting the leukocyte-endothelium interaction and neutrophil transmigration during inflammation in a dose dependent manner (Nunes et al, 2009).…”

Section: Role Of Plant Lectins In Inflammatory Diseasesmentioning

confidence: 99%

Structure-function and application of plant lectins in disease biology and immunity

Mishra

Behura

Mawatwal

et al. 2019

Food and Chemical Toxicology

156

View full text Add to dashboard Cite

A B S T R A C TLectins are proteins with a high degree of stereospecificity to recognize various sugar structures and form reversible linkages upon interaction with glyco-conjugate complexes. These are abundantly found in plants, animals and many other species and are known to agglutinate various blood groups of erythrocytes. Further, due to the unique carbohydrate recognition property, lectins have been extensively used in many biological functions that make use of protein-carbohydrate recognition like detection, isolation and characterization of glycoconjugates, histochemistry of cells and tissues, tumor cell recognition and many more. In this review, we have summarized the immunomodulatory effects of plant lectins and their effects against diseases, including antimicrobial action. We found that many plant lectins mediate its microbicidal activity by triggering host immune responses that result in the release of several cytokines followed by activation of effector mechanism. Moreover, certain lectins also enhance the phagocytic activity of macrophages during microbial infections. Lectins along with heat killed microbes can act as vaccine to provide long term protection from deadly microbes. Hence, lectin based therapy can be used as a better substitute to fight microbial diseases efficiently in future.

show abstract

“…Zymosan, a polysaccharide derived from yeast cell walls, could induce severe and erosive arthritis and severe pain once injected (do Val et al, 2014). Carrageenan and mustard oil could induce plasma protein extravasation and leukocyte migration; mustard oil simultaneously causes in neuron depolarization and neurogenic inflammation, whereas carrageenan represents the non-neurogenic one (Clemente-Napimoga et al, 2019). The vibrissal pad injection of complete Freund's adjuvant (CFA) has also been developed as a reliable model for TMD study (Luo et al, 2020).…”

Section: Bioactive Compounds In Preclinical Studiesmentioning

confidence: 99%

Therapeutic Agents for the Treatment of Temporomandibular Joint Disorders: Progress and Perspective

Cai

et al. 2021

Front. Pharmacol.

View full text Add to dashboard Cite

Temporomandibular joint disorders (TMD) are a common health condition caused by the structural or functional disorders of masticatory muscles and the temporomandibular joint (TMJ). Abnormal mandibular movement in TMD patients may cause pain, chronic inflammation, and other discomfort, which could be relieved by a variety of drugs through various delivery systems. In this study, we summarized commonly used therapeutic agents in the management of TMD as well as novel bioactive molecules in preclinical stage and clinical trials. The emerging therapy strategies such as novel intra-TMJ delivery systems and implants based on tissue engineering are also discussed. This comprehensive review will strengthen our understanding of pharmacological approaches for TMD therapy.

show abstract

Dioclea violacea lectin ameliorates inflammation in the temporomandibular joint of rats by suppressing intercellular adhesion molecule-1 expression

Cited by 16 publications

References 70 publications

Improved efficacy of naproxen-loaded NLC for temporomandibular joint administration

Improved efficacy of naproxen-loaded NLC for temporomandibular joint administration

Structure-function and application of plant lectins in disease biology and immunity

Therapeutic Agents for the Treatment of Temporomandibular Joint Disorders: Progress and Perspective

Contact Info

Product

Resources

About