Diphenyl diselenide derivatives inhibit microbial biofilm formation involved in wound infection

Abstract

BackgroundOrganoselenium compounds have antimicrobial activity against some bacteria and fungi; furthermore, the antioxidant activity of diselenides has been demonstrated. The aim of the present work was to examine the in vitro minimal inhibitory concentration of a panel of differently substituted diselenides and their effectiveness in inhibiting biofilm formation and dispersing preformed microbial biofilm of Staphylococcus epidermidis, Staphylococcus aureus, Streptococcus pyogenes and Pseudomonas aeruginosa and the yeast Candida albicans, all involved in wound infections. Moreover, the cytotoxicity of the compounds was determined in human dermal fibroblast and keratinocytes. In closing, we tested their direct antioxidant activity.ResultsDiselenides showed different antimicrobial activity, depending on the microorganism. All diselenides demonstrated a good antibiofilm activity against S. aureus and S. epidermidis, the compounds camphor diselenide, bis[ethyl-N-(2’-selenobenzoyl) glycinate] and bis[2’-seleno-N-(1-methyl-2-phenylethyl) benzamide] were active against S. pyogenes and C. albicans biofilm while only diselenides 2,2’-diselenidyldibenzoic acid and bis[ethyl-N-(2’-selenobenzoyl) glycinate] were effective against P. aeruginosa. Moreover, the compounds bis[ethyl-N-(2’-selenobenzoyl) glycinate] and bis[2’-seleno-N-(1-methyl-2-phenylethyl) benzamide] showed an antioxidant activity at concentrations lower than the 50 % of cytotoxic concentration.ConclusionsBecause microbial biofilms are implicated in chronic infection of wounds and treatment failure, the combination of antimicrobial activity and potential radical scavenging effects may contribute to the improvement of wound healing. Therefore, this study suggests that bis[ethylN-(2’-selenobenzoyl) glycinate] and bis[2’-seleno-N-(1-methyl-2-phenylethyl) benzamide] are promising compounds to be used in preventing and treating microbial wound infections.

Antibiofilm and Antiadhesive Activities of Different Synbiotics

“…The in vitro static biofilm assay was performed using a 96-well microtiter plate as previously described with some modification [16,17]. To grow biofilms, overnight cultures of S. aureus and E. coli were diluted 1:100 into 15 ml of MHB supplemented with 2% w/v sucrose (Sigma-Aldrich, Ottawa, Canada, USA), in presence or in absence of different probiotic supernatants.…”

Investigation on the effect of known potent S. aureus NorA efflux pump inhibitors on the staphylococcal biofilm formation

“…39,40 To grow biolms, overnight cultures of S. aureus or S. epidermidis were diluted 1 : 100 (corresponding to 1-2 Â 10 6 CFU mL À1 ) in TSB supplemented with 2% sucrose. Bacteria were incubated in the presence or absence of different NorA EPIs (1a-c 32,33 and 2…”

Antioxidant effect of quinoline derivatives containing or not selenium: Relationship with antinociceptive action quinolines are antioxidant and antinociceptive

“…In this sense it is important to use antioxidant molecules that decrease oxidative stress, reducing the incidence of various diseases (Gay et al 2010, Maes et al 2011, Gupta et al 2014. It is therefore important to highlight organoselenium compounds, which exhibit different pharmacological properties, such as antioxidant action (Nogueira and Rocha 2010, Luchese et al 2012, Sancineto et al 2016). Parallel to organoselenium compounds are quinoline derivatives.…”