Diplocloster agilis gen. nov., sp. nov. and Diplocloster modestus sp. nov., two novel anaerobic fermentative members of Lachnospiraceae isolated from human faeces

Abstract

Three novel strains of Gram-stain-negative, obligately anaerobic, spore-forming straight or slightly curved rods with pointed ends occurring singly or in pairs were isolated from the faeces of healthy human children. The strains were characterized by mesophilic fermentative metabolism and production of acetate, ethanol and H2 as the end metabolic products. Strains ASD3451 and ASD5720T were motile, fermented lactose and raffinose, and weakly fermented maltose. Strain ASD4241T was non-motile and did not ferment the carbohydrates listed above but fermented starch. Strains ASD3451 and ASD5720T shared average nucleotide identity higher than 98.5 % with each other, while ASD4241T had only 88.5-89 % identity to them. Based on phylogenetic and chemotaxonomic analyses, we propose Diplocloster agilis gen. nov., sp. nov. (ASD5720T=JCM 34353T=VKM B-3497T) and Diplocloster modestus sp. nov. (ASD4241T=JCM 34351T=VKM B-3498T) within the family Lachnospiraceae .

Anaerolentibacter hominis gen. nov. sp. nov., Diplocloster hominis sp. nov. and Pilosibacter fragilis gen. nov. sp. nov., isolated from human faeces

“…Diplocloster is a genus within Lachnospiraceae composed of two species, namely Diplocloster agilis and Diplocloster modestus . They are Gram-negative, obligately anaerobic, spore-forming, straight or slightly curved rod-shaped with pointed ends, and occur singly [18].…”

“…Comparison of the phenotypic characteristics between strain CSJ-3 T and its closely related type strains Strains: 1, CSJ-3 T (data from this study); 2, Diplocloster modestus ASD 4241 T[18]; 3, Diplocloster agilis ASD 5720 T[18]. +, Positive; -, negative; nd, no data available.…”