Dipyridamole enhances inhibitory effect of adenosine on neutrophils in human peripheral blood

Zhang, Qingyan; Zhang, Jing; Tian, Qian; Li, Minghua

doi:10.1016/s1007-4376(08)60072-6

Cited by 8 publications

(10 citation statements)

References 4 publications

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“…In contrast, activation of the A 2A receptor by a high concentration of adenosine attenuates neutrophil chemotaxis in response to IL-8. Our findings are consistent with previous studies indicating that the high affinity A 1 receptor promotes neutrophil chemotaxis, whereas the lower affinity A 2A receptor limits neutrophil chemotaxis [25,27,29] . This likely reflects the different signalling pathways linked to these receptors: A 1 is G i/o -coupled and the occupancy of A 1 diminishes cAMP accumulation, whereas A 2A is G s -coupled and the binding of adenosine to the A 2A receptor increases the formation of cAMP [29] .…”

Section: Discussionsupporting

confidence: 93%

“…A similar result was also observed by adding adenosine (10 − 9 M to 10 − 6 M) in the lower wells with fMLP in a chemotaxis assay [26] . In addition, adenosine had a similar effect on human monocytes [27] . Analogous findings have been reported also for ATP, from which adenosine is derived by intracellular and extracellular breakdown [26,28] .…”

Section: Discussionmentioning

confidence: 76%

“…These researchers proposed that this mechanism is mediated by increased adenosine levels. Another study suggested that dipyridamole enhanced the inhibitory effects of adenosine, which, in turn, reduced the effect of fMLP-activated neutrophil hydrogen peroxide (H 2 O 2 ) production [27] .…”

Section: Discussionmentioning

confidence: 99%

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Ticagrelor potentiates adenosine-induced stimulation of neutrophil chemotaxis and phagocytosis

Alsharif

Thomas

Judge

et al. 2015

Vascular Pharmacology

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In the PLATO study, ticagrelor was associated with fewer pulmonary infections and subsequent deaths than clopidogrel. Neutrophils are a first-line defence against bacterial lung infection; ticagrelor inhibits cellular uptake of adenosine, a known regulator of neutrophil chemotaxis and phagocytosis. We assessed whether the inhibition of adenosine uptake by ticagrelor influences neutrophil chemotaxis and phagocytosis. Neutrophils and erythrocytes were isolated from healthy volunteers. Concentration-dependent effects of adenosine on IL-8-induced neutrophil chemotaxis were investigated and the involved receptors identified using adenosine receptor antagonists. The modulatory effects of ticagrelor on adenosine-mediated changes in neutrophil chemotaxis and phagocytosis of Streptococcus pneumoniae were determined in the presence of erythrocytes to replicate physiological conditions of cellular adenosine uptake. Low-concentration adenosine (10− 8 M) significantly increased IL-8-induced neutrophil chemotaxis (% neutrophil chemotaxis: adenosine 28.7% ± 4.4 vs. control 22.6% ± 2.4; p < 0.01) by acting on the high-affinity A1 receptor. Erythrocytes attenuated the effect of adenosine, although this was preserved by ticagrelor and dipyridamole (another inhibitor of adenosine uptake) but not by control or by cangrelor. Similarly, in the presence of erythrocytes, a low concentration of adenosine (10− 8 M) significantly increased neutrophil phagocytic index compared to control when ticagrelor was present (37.6 ± 6.6 vs. 28.0 ± 6.6; p = 0.028) but had no effect in the absence of ticagrelor. We therefore conclude that the inhibition of cellular adenosine reuptake by ticagrelor potentiates the effects of a nanomolar concentration of adenosine on neutrophil chemotaxis and phagocytosis. This represents a potential mechanism by which ticagrelor could influence host defence against bacterial lung infection.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 76%

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Ticagrelor potentiates adenosine-induced stimulation of neutrophil chemotaxis and phagocytosis

Alsharif

Thomas

Judge

et al. 2015

Vascular Pharmacology

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“…In the BLASTn alignment, based on ITS sequences, Coprinopsis pusilla and C. melanthina KC992961 ( Örstadius et al 2015 ) had the highest sequence identities (94.92%–95.26%), with 34–36 base differences. Corpinopsis jilinensis and C. uliginicola MG712323 ( Zhang 2019 ) had the highest sequence identity (99.27%), with five base differences. The sequence identities between C. jilinensis and C. uliginicola KC992960 Type ( Örstadius et al 2015 ) were 93.98%–94.35%, with 39–42 base differences.…”

Section: Resultsmentioning

confidence: 99%

Two new psathyrelloid species of Coprinopsis (Agaricales, Psathyrellaceae) from China

Rao

Dai

Zhao

et al. 2021

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In this study, Coprinopsis jilinensis and Coprinopsis pusilla were introduced, based on their morphological characteristics, the internal transcribed spacer (ITS) and large subunit ribosomal (LSU) region sequences of nrDNA. These new psathyrelloid species were found in Jilin Province, China. Coprinopsis jilinensis has brown pileus, utriform pleurocystidia, brown, smooth, dextrinoid basidiospores and tiny pore. It mainly grows on humus. Coprinopsis pusilla has small basidiomata, greyish-white pileus, thick and distinct veil at edges, subcolourless and verrucose basidiospores. It is poreless and it grows on the decaying wood of broad-leaved trees. Both of them belong to the C. sect. Melanthinae. A supplementary description of C. sect. Melanthinae was given in combination with the newly-discovered taxa and an identification key to the fourteen psathyrelloid species of Coprinopsis is provided. Coprinopsis sect. Canocipes and C. sect. Quartoconatae were evaluated and the phylogenetic position of the psathyrelloid species of Coprinopsis was discussed. Psathyrella subagraria, as a confusing species, was also discussed in this study.

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“…The volatile oil is one of the major bioactive components in Chrysanthemum morifolium. At present, the main extraction methods of volatile oil from Chrysanthemum morifolium are direct distillation extraction method 6,7 and ultrasonic extraction method 8 , etc. And the major analysis methods are GC-MS 9 and GC × GC/TOFMS 10 , etc.…”

Section: Introductionmentioning

confidence: 99%

Component Analysis of Volatile Oil from Chrysanthemum morifolium by Supercritical Carbon Dioxide Extraction

Wen¹,

Jin²

2014

Asian J. Chem.

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In this study, the volatile oil was extracted from Chrysanthemum morifolium by supercritical carbon dioxide extraction. Ninety three peaks were separated and 26 kinds of compounds were identified by GC-MS and their relative contents were determined by area normalization method. The main components in Chrysanthemum morifolium are monoterpenes hydrocarbons, sesquiterpenes hydrocarbons and their oxo-derivatives, etc.

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Dipyridamole enhances inhibitory effect of adenosine on neutrophils in human peripheral blood

Cited by 8 publications

References 4 publications

Ticagrelor potentiates adenosine-induced stimulation of neutrophil chemotaxis and phagocytosis

Ticagrelor potentiates adenosine-induced stimulation of neutrophil chemotaxis and phagocytosis

Two new psathyrelloid species of Coprinopsis (Agaricales, Psathyrellaceae) from China

Component Analysis of Volatile Oil from Chrysanthemum morifolium by Supercritical Carbon Dioxide Extraction

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