Direct addition of cultures of tester bacteria into semi-permeable membrane devices (SPMDs) as a modified procedure for preliminary detection of mutagenic pollution of the marine environment by use of microbiological mutagenicity assays

Comparison of the use of mussels and semipermeable membrane devices for monitoring and assessment of accumulation of mutagenic pollutants in marine environment in combination with a novel microbiological mutagenicity assay

“…The assay was performed according to Chęć et al (2006). Briefly, the 91.4 cm long polyethylene tube (2.5 cm×70-95 μm wall thickness) containing 1 ml of trioleine (99% purity) (ExposMeter Sampling Technologies, Sweden), was cut into 10 cm lengths and sealed on both ends.…”

“…Since the combination of SPMDs with the V. harveyi bioluminescence mutagenicity assay has been optimized previously (Chęć et al 2006), no preliminary assays were necessary in this case. However, effects of various amounts of a homogenate of mussels' tissues had to be tested in order to assess any influence on bacterial bioluminescence and to optimize the method.…”

“…Moreover, a modified V. harveyi mutagenicity assay, based on bioluminescence induction, has been described recently . For testing mutagenic pollution of environment, this assay may be used in combination with SPMDs by direct addition of cultures of tester bacteria into the devices (Chęć et al 2006). The aim of this work was to compare the use of mussels and SPMDs in combination with the V. harveyi bioluminescence mutagenicity assay to detect accumulation of mutagens in marine environment.…”