Direct analysis of biological samples by total reflection X-ray fluorescence

Marco, Musiani; Hernández-Caraballo, Edwin A.

doi:10.1016/j.sab.2004.05.017

Cited by 45 publications

(10 citation statements)

References 42 publications

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“…With the development of semiconductor detectors able to discriminate X-ray energies, the development of the energydispersive XRF technique, and the availability of less expensive instrumentation, more practical use of XRF became possible [166]. The conventional energy-dispersive XRF technique uses a combination of about 45°/45°for the incident and takeoff angles, whereas the TXRF technique uses a combination of angles of 0.1°and 90° [165,167,168]. The TXRF technique results in significant reduction in the spectral background because a very small part of the primary beam penetrates into the sample.…”

Section: Neutron Activation Analysismentioning

confidence: 99%

“…The TXRF technique results in significant reduction in the spectral background because a very small part of the primary beam penetrates into the sample. In addition, a doubled fluorescence intensity signal is observed because the sample is excited by both the incident beam and the reflected beam [167]. Moreover, in the TXRF technique a very small sample amount is used (1 ng to 100 μg).…”

Section: Neutron Activation Analysismentioning

confidence: 99%

“…Halogens, especially chlorine, bromine, and iodine can be determined in biological samples using TXRF, and suitable LODs (microgram per liter range) can be obtained [75,167]. However, for fluorine, the fluorescence yield and the absorption of radiation in the beam pathway is very low and consequently poor LODs (about 120 μg g −1 ) are obtained [170,171].…”

Section: Neutron Activation Analysismentioning

confidence: 99%

“…On the other hand, works using TXRF to determine iodine are scarce in the literature [75], and some approaches are required to minimize interferences when biological samples are being analyzed, such as acidic microwave digestion and slurry sampling [167,172]. However, digestion of small samples demands careful control of the digestion conditions in order to avoid contamination, volatilization losses, etc.…”

Section: Neutron Activation Analysismentioning

confidence: 99%

See 3 more Smart Citations

Analytical methods for the determination of halogens in bioanalytical sciences: a review

et al. 2013

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Fluorine, chlorine, bromine, and iodine have been studied in biological samples and other related matrices owing to the need to understand the biochemical effects in living organisms. In this review, the works published in last 20 years are covered, and the main topics related to sample preparation methods and analytical techniques commonly used for fluorine, chlorine, bromine, and iodine determination in biological samples, food, drugs, and plants used as food or with medical applications are discussed. The commonest sample preparation methods, as extraction and decomposition using combustion and pyrohydrolysis, are reviewed, as well as spectrometric and electroanalytical techniques, spectrophotometry, total reflection X-ray fluorescence, neutron activation analysis, and separation systems using chromatography and electrophoresis. On this aspect, the main analytical challenges and drawbacks are highlighted. A discussion related to the availability of certified reference materials for evaluation of accuracy is also included, as well as a discussion of the official methods used as references for the determination of halogens in the samples covered in this review.

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Section: Neutron Activation Analysismentioning

confidence: 99%

Section: Neutron Activation Analysismentioning

confidence: 99%

Section: Neutron Activation Analysismentioning

confidence: 99%

Section: Neutron Activation Analysismentioning

confidence: 99%

See 2 more Smart Citations

Analytical methods for the determination of halogens in bioanalytical sciences: a review

et al. 2013

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“…The critical point is the thickness of the sample layer after any type of sample preparation process [7]. In order to remove the organic matrix, different sample preparation procedures were developed and published during the last decade: microwave digestion [8], ashing [9], chemical modification [10] and instrumental modification [11].…”

Section: Introductionmentioning

confidence: 99%

Direct elemental analysis of cancer cell lines by total reflection X-ray fluorescence

Szoboszlai

Réti

Budai

et al. 2008

Spectrochimica Acta Part B: Atomic Spectroscopy

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Advantages of Total Reflection X‐Ray Fluorescence Analysis Over Conventional Trace Analytical Techniques in Different Areas of Material Characterization

Mishra

2024

Encyclopedia of Analytical Chemistry

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Total reflection X‐ray fluorescence (TXRF) spectrometry, a variant of energy‐dispersive X‐ray fluorescence (EDXRF), has its detection limits comparable with conventional trace elemental determination techniques, e.g. inductively coupled plasma mass spectrometry (ICP‐MS) and inductively coupled plasma – atomic emission spectrometry (ICP‐AES). In addition, it has several advantages over these techniques, e.g. requirement of only a few microliters of sample (or picogram level of analytes), possibility of determination of metal and nonmetals alike, analysis of solids without dissolving (either rubbing on TXRF supports or making slurry), determination of low‐atomic‐number elements, and wide elemental range from C to Pu with modification of instrumental and sample preparation conditions. Due to these advantages, TXRF analysis is preferred over ICP‐MS or ICP‐AES in those areas where sample amount available is in minute quantity, dissolution of sample is difficult or not possible, sample dissolution is not preferred, or sample analysis is to be rechecked on same specimen in case of a doubt. There are several such studies reported on preferential application of TXRF in forensic, environmental, biological, agricultural, nuclear and several other areas. Some of these applications include analysis of gunshot residues (GSR), biological samples, and plutonium analysis for major and trace elements where sample amount available or preferred is very small. Similarly studies on TXRF applications for analysis of sintered (U,Th)O 2 pellets, soil samples, milk samples, tissue cells, etc. are also reported in various publications. Direct elemental analysis of environmental samples, e.g. aerosol particles, is another preferential area of TXRF spectrometry. Some of these studies are described in the present article.

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Direct analysis of biological samples by total reflection X-ray fluorescence

Cited by 45 publications

References 42 publications

Analytical methods for the determination of halogens in bioanalytical sciences: a review

Analytical methods for the determination of halogens in bioanalytical sciences: a review

Direct elemental analysis of cancer cell lines by total reflection X-ray fluorescence

Advantages of Total Reflection X‐Ray Fluorescence Analysis Over Conventional Trace Analytical Techniques in Different Areas of Material Characterization

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