Direct analysis of trans-resveratrol in red wine by high performance liquid chromatography with chemiluminescent detection

Zhou, Jian; Cui, Hua; Wan, Guohui; Hong, Xu; Yongqiang, Pang; Duan, Chunfeng

doi:10.1016/j.foodchem.2004.05.003

Cited by 51 publications

(23 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Taking into account the physicochemical properties of the target compounds, high-performance liquid chromatography (HPLC) is the technique most commonly used, employing different detection modes like ultraviolet or diode array (Cvejic et al 2010;Zhou et al 2007;Du et al 2007;Enguo et al 2007;Abert Vian et al 2005), chemiluminescence (Zhou et al 2004), electrochemistry (Zhang et al 2007; Kahoun et al 2008), fluorimetry (Grippi et al 2008;Rodríguez-Delgado et al 2001), or mass spectrometry (Sun et al 2006;Hamm et al 2010;Jaitz et al 2010;Nicoletti et al 2007;Counet et al 2006;Montsko et al 2008). As an alternative to HPLC, gas chromatography-mass spectrometry (GC-MS) (Soleas et al 2001;Luan et al 2000) and capillary electrophoresis (Spanilá et al 2005;Zhang et al 2009;Orlandini et al 2008) have also been employed.…”

Section: Introductionmentioning

confidence: 99%

Liquid Chromatographic Determination of Resveratrol and Piceid Isomers in Honey

et al. 2011

View full text Add to dashboard Cite

In this work, a liquid chromatography method with diode array and fluorescence detection and electrospray ionization mass spectrometry (LC-DAD-FLD and LC-ESI-MS) has been developed for the determination of residues of resveratrol in all its forms (free isomers and glycosylates) in honey. This procedure involves a solidphase extraction on polymeric cartridges for the isolation of trans/cis-isomers of resveratrol and piceid from diluted honey samples. Chromatographic separation of the analytes was performed in isocratic mode on a C 18 column (150 mm×4.6 mm i.d., 5 μm) operated at 30°C. The mobile phase consisted of a mixture of water with 1% formic acid and acetonitrile (75/25, v/v), and the flow rate was set at 1.0 mL/min. Average analyte recoveries were from 71% to 113% in replica sets of fortified honey samples. The detection limits when using LC-DAD-FLD were between 40 and 120 μg/kg, meanwhile for LC-ESI-MS were between 1.4 and 14.6 μg/kg. The proposed method was applied to the analysis of honey samples collected from treated beehives in experimental apiaries after a preliminary field trial, and they were not found any residues over the detection limits of the studied compounds.

show abstract

Section: Introductionmentioning

confidence: 99%

Liquid Chromatographic Determination of Resveratrol and Piceid Isomers in Honey

et al. 2011

View full text Add to dashboard Cite

show abstract

“…3 These demonstrate that this compound has beneficial effects on human health, which explains the interest in its determination that has arisen in recent years. High performance liquid chromatography (HPLC), [4][5][6][7] capillary electrophoresis, [8][9] UV and fluorescence, [10][11] and various other techniques have been employed for the determination of resveratrol in liquid samples such as wines and other drinks. But complicated preconcentration is also coupled with these techniques due to the complexity of the real samples and the low concentration of the analyte.…”

Section: Introductionmentioning

confidence: 99%

Adsorptive Voltammetric Behaviors of Resveratrol at Graphite Electrode and its Determination in Tablet Dosage Form

Liu

Wang

et al. 2008

J Chinese Chemical Soc

View full text Add to dashboard Cite

The adsorptive voltammetric behavior of resveratrol was studied at a graphite electrode in B-R buffer (pH = 6.0) solution using adsorptive cyclic voltammetric technique. The oxidation of resveratrol is an irreversible adsorption controlled process. The oxidation mechanism was proposed and discussed in this work. The dependence of the current on pH and the concentration and nature of buffer were investigated to optimize the experimental conditions for the determination of resveratrol. It was found that in the range of 8.0´10-9~2 .0´10 -6 mol/L, the currents measured by differential pulse voltammetries presented a good linear property as a function of the concentrations of resveratrol. In addition, validation parameters, such as reproducibility, sensitivity and recovery were evaluated as well. The proposed method was also successfully applied for the determination of resveratrol in Chinese patent medicine with good results.

show abstract

“…The LOD for the method reported in this paper is 10 or even 100 times lower than these. There is only one reported HPLC method with chemiluminiscent detection in which a LOD of 0.2 lg/L has been reached [24].…”

Section: Discussionmentioning

confidence: 99%

“…In recent years, reverse phase high performance liquid chromatography with acidic solvent gradient elution has been the most commonly used procedure for the determination of resveratrol and/or piceid in wine, with either diode array detection (DAD) [16 -19], fluorimetric detection (FLD) [20,21], DAD -FLD [22,23], chemiluminescent detection (CLD) [24,25], electrochemical detection (ECD) [23], or mass spectrometry (MS) [23] and DAD -MS [26]. Detection based on electrochemistry, luminescence, and MS offers higher sensitivity than DAD.…”

Section: Introductionmentioning

confidence: 99%

Isocratic chromatography of resveratrol and piceid after previous generation of fluorescent photoproducts: Wine analysis without sample preparation

Galeano-Dı́az

Durán-Merás

Airado‐Rodríguez

2007

J of Separation Science

View full text Add to dashboard Cite

Resveratrol and its 3-glucoside (piceid), are stilbene-like molecules produced by plants. Both of them are weakly fluorescent, but highly fluorescent compounds are obtained when their hydroethanolic solutions are UV-irradiated, which implies a substantial improvement in the sensitivity of analytical methods. Experimental design (central composite design) together with the response surface methodology have been used to find optimum conditions for the fast, sensitive, and precise chromatographic analysis (with isocratic elution) of resveratrol and piceid in wine samples. These compounds have been UV-transformed into their respective photoproducts, which have been separated in a C18 column (Novapack C(18) 150x3.9 mm, 4 microm) by isocratic elution, using a mobile phase made up of acetonitrile and 4.1 vol% aqueous acetic acid, 19:81 v/v, at a flow rate of 0.8 mL/min, and fluorimetrically detected at 364 nm (lambda(exc) = 260 nm). Detection limits (S/N = 3) are 0.29 and 0.28 microg/L for resveratrol and piceid, respectively. The method has been applied to the analysis of these compounds in wine samples without a clean-up step. The analysis is completed in only 20 min. The standard addition method has been applied to the analysis of a commercial red wine and average recoveries near 100% were obtained for resveratrol and piceid. Three wine pools were satisfactorily analysed by external calibration.

show abstract

Direct analysis of trans-resveratrol in red wine by high performance liquid chromatography with chemiluminescent detection

Cited by 51 publications

References 31 publications

Liquid Chromatographic Determination of Resveratrol and Piceid Isomers in Honey

Liquid Chromatographic Determination of Resveratrol and Piceid Isomers in Honey

Adsorptive Voltammetric Behaviors of Resveratrol at Graphite Electrode and its Determination in Tablet Dosage Form

Isocratic chromatography of resveratrol and piceid after previous generation of fluorescent photoproducts: Wine analysis without sample preparation

Contact Info

Product

Resources

About