Liquid Chromatographic Determination of Resveratrol and Piceid Isomers in Honey

Abstract

In this work, a liquid chromatography method with diode array and fluorescence detection and electrospray ionization mass spectrometry (LC-DAD-FLD and LC-ESI-MS) has been developed for the determination of residues of resveratrol in all its forms (free isomers and glycosylates) in honey. This procedure involves a solidphase extraction on polymeric cartridges for the isolation of trans/cis-isomers of resveratrol and piceid from diluted honey samples. Chromatographic separation of the analytes was performed in isocratic mode on a C 18 column (150 mm×4.6 mm i.d., 5 μm) operated at 30°C. The mobile phase consisted of a mixture of water with 1% formic acid and acetonitrile (75/25, v/v), and the flow rate was set at 1.0 mL/min. Average analyte recoveries were from 71% to 113% in replica sets of fortified honey samples. The detection limits when using LC-DAD-FLD were between 40 and 120 μg/kg, meanwhile for LC-ESI-MS were between 1.4 and 14.6 μg/kg. The proposed method was applied to the analysis of honey samples collected from treated beehives in experimental apiaries after a preliminary field trial, and they were not found any residues over the detection limits of the studied compounds.

Development and validation of a liquid chromatography with mass spectrometry method to determine resveratrol and piceid isomers in beeswax

“…We decided to start separation optimization by using the chromatographic conditions that were proposed in our previous study in which stilbenes were analyzed in honey. The analytical column was a Gemini C 18 (150 × 4.6 mm), and the mobile phase was a mixture of formic acid in water (1%, v/v; pH 2.1) and acetonitrile at at a flow rate of 1 mL/min in isocratic elution mode.…”

“…Stock standard solutions of each trans compound, at a concentration of 1000 mg/L, were prepared in a mixture of water and methanol (50:50, v/v). The cis isomers were obtained from trans isomers with the same procedure developed in a previous publication . Briefly, mixtures of trans compounds were irradiated for 24 h with a Cole‐Palmer B100APUV lamp at 254 nm.…”

“…We have decided to investigate the potential presence of these compounds in beeswax, as it could be considered a beehive reservoir, and the compounds present in beeswax could directly affect the bee colony or be transmitted to other bee products, and in the case of the stilbenes, with positive health effects. Resveratrol and its related compounds have been studied in only one bee matrix, namely, honey . The methodology involved SPE of the analytes before analysis by LC.…”

“…The determination of resveratrol and its related compounds has usually been achieved by means of LC in RP mode (C 18 columns) with UV or diode array , fluorescence , or MS detectors . It should be also mentioned that the hyphenation of RP‐LC and zwitterionic hydrophilic interaction LC with MS has been successfully employed in the last years for the analysis of phenols, including cis ‐ and trans ‐resveratrol .…”

Fast Separation and Determination of Flavonoids in Honey Samples by Capillary Zone Electrophoresis

“…18,19,20 The geometric structures of flavonoids are composed of two phenyl rings and a heterocyclic ring, which is the basic structure. There are often different substituents on the basic structure, such as hydroxyl.…”