Direct and <i>in vitro</i> observation of growth hormone receptor molecules in A549 human lung epithelial cells by nanodiamond labeling

Cheng, Chi‐Yuan; Perevedentseva, E.; Tu, Jhih-Sian; Chung, Pei‐Hua; Cheng, Caleb; Liu, Kuang Kai; Ji, Chao; Chen, Po-Hsiang; Chang, Chia-Ching

doi:10.1063/1.2727557

Cited by 115 publications

(67 citation statements)

References 22 publications

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“…Such cNDs, especially detonation NDs, have been functionalised with biomolecules through either adsorption by electrostatic and/or hydrophobic interactions [16][17][18][19] or covalent linkage. [16,[20][21][22] In a few reports, the activities and/or the intracellular locations of these conjugated NDs were analysed. [17,18,22] One of the most critical points that has limited the use of NDs in biological applications is the preparation of a functionalised cND colloidal suspension that is stable over long periods of time.…”

Section: Introductionmentioning

confidence: 99%

Peptide‐Grafted Nanodiamonds: Preparation, Cytotoxicity and Uptake in Cells

et al. 2008

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Nanodiamonds that were prepared by high pressure/high temperature were functionalized with biomolecules for biological applications. Nanodiamonds (NDs, < or =35 nm) that were coated by silanization or with polyelectrolyte layers were grafted with a fluorescent thiolated peptide via a maleimido function; this led to an aqueous colloidal suspension that was stable for months. These substituted NDs were not cytotoxic for CHO cells. Their capacity to enter mammalian cells, and their localisation inside were ascertained after labelling the nucleus and actin, by examining the cells by confocal, reflected light and fluorescence microscopy.

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Section: Introductionmentioning

confidence: 99%

Peptide‐Grafted Nanodiamonds: Preparation, Cytotoxicity and Uptake in Cells

et al. 2008

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“…For example, Weng et al (2009) found that transferrin-conjugated 100-nm diamonds were taken up via the abundant transferrin receptors on HeLa cells in a clathrin-dependant manner. In contrast, Cheng et al (2007) observed that similar-sized nanodiamonds conjugated to growth hormone remained extracellular, presumably attached to the binding domain of the growth hormone receptor on A549 cells, while the unconjugated control nanodiamonds were internalised. Nanodiamonds can also be internalised by other receptormediated pathways.…”

Section: Bio-compatibility Of Nanodiamondmentioning

confidence: 81%

Luminescent nanodiamonds for biomedical applications

et al. 2011

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In recent years, nanodiamonds have emerged from primarily an industrial and mechanical applications base, to potentially underpinning sophisticated new technologies in biomedical and quantum science. Nanodiamonds are relatively inexpensive, biocompatible, easy to surface functionalise and optically stable. This combination of physical properties are ideally suited to biological applications, including intracellular labelling and tracking, extracellular drug delivery and adsorptive detection of bioactive molecules. Here we describe some of the methods and challenges for processing nanodiamond materials, detection schemes and some of the leading applications currently under investigation.

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“…cancer cells by identifying the sp 3 carbon Raman signals of NDs. 146 Growth hormone-ND complexes were created by carboxylating and covalently linking 100 nm NDs to the growth hormone. 146 When mapped by confocal Raman spectrophotometry, the NDs bound to growth hormone were located on the surface of cells, while the unconjugated carboxylated NDs were internalized into the cells.…”

Section: Elastic and Inelastic Light Scattering Imagingmentioning

confidence: 99%

Nanodiamonds as novel nanomaterials for biomedical applications: drug delivery and imaging systems

Kaur¹,

Badea²

2013

IJN

104

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Detonation nanodiamonds (NDs) are emerging as delivery vehicles for small chemical drugs and macromolecular biotechnology products due to their primary particle size of 4 to 5 nm, stable inert core, reactive surface, and ability to form hydrogels. Nanoprobe technology capitalizes on the intrinsic fluorescence, high refractive index, and unique Raman signal of the NDs, rendering them attractive for in vitro and in vivo imaging applications. This review provides a brief introduction of the various types of NDs and describes the development of procedures that have led to stable single-digit-sized ND dispersions, a crucial feature for drug delivery systems and nanoprobes. Various approaches used for functionalizing the surface of NDs are highlighted, along with a discussion of their biocompatibility status. The utilization of NDs to provide sustained release and improve the dispersion of hydrophobic molecules, of which chemotherapeutic drugs are the most investigated, is described. The prospects of improving the intracellular delivery of nucleic acids by using NDs as a platform are exemplified. The photoluminescent and optical scattering properties of NDs, together with their applications in cellular labeling, are also reviewed. Considering the progress that has been made in understanding the properties of NDs, they can be envisioned as highly efficient drug delivery and imaging biomaterials for use in animals and humans.

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Direct and in vitro observation of growth hormone receptor molecules in A549 human lung epithelial cells by nanodiamond labeling

Cited by 115 publications

References 22 publications

Peptide‐Grafted Nanodiamonds: Preparation, Cytotoxicity and Uptake in Cells

Peptide‐Grafted Nanodiamonds: Preparation, Cytotoxicity and Uptake in Cells

Luminescent nanodiamonds for biomedical applications

Nanodiamonds as novel nanomaterials for biomedical applications: drug delivery and imaging systems

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