Direct axillary shoot regeneration from the mature seed explant of the hairy vetch (Vicia villosa Roth)

Aasım, Muhammad; Sahin-Demirbag, Nurdan; Khawar, Mahmood Khalid; Kendir, Hayrettin; Özcan, Sebahattin

doi:10.2298/abs1103757a

Cited by 11 publications

(8 citation statements)

References 6 publications

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“…2b). Our results are in accordance with that of Aasim et al (2011) who achieved direct shoot regeneration from seed explants of Vicia villosa Roth on MS medium supplemented with Thidiazuron and IBA in combination. Ghosh and Sen (1994) also obtained maximum shoot regeneration from shoot tip explants of Asparagus cooperi Baker on MS medium supplemented with BAP in combination with NAA.…”

Section: B Multiple Shoot Regeneration From Seed Explantsupporting

confidence: 92%

In vitro Propagation of Polygonatum verticillatum All. A Threatened Medicinal Herb through Seed Explant

Qadir¹,

Singh²,

Kour³

et al. 2020

JSR

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To conserve and overcome the limitations of traditional propagation, a need was felt to develop an in vitro propagation protocol for Polygonatum verticillatum (L.) All. by using seeds as explant. The present study was undertaken to evaluate the most suitable concentration of plant growth regulators on callus induction and subsequent rhizome and shoot regeneration. Murashige and Skoog (MS) medium fortified with Kn (8mg/l) in combination with IAA (2mg/l) proved most effective in producing maximum amount of callus from in vitro sprouted seed explants which were pre-treated with GA3 (1 mg/l) and kept at-20 º C for 30 days. Subculturing of callus on MS medium fortified with BAP (4mg/l) and NAA (1mg/l) in combination regenerated rhizomes. Shoots regenerated (13.25±2.32) from rhizomes on MS medium fortified with Kn (2mg/l) in combination with BAP (2mg/l). Roots (1.93±0.34 cm) regenerated from shoots inoculated on full strength MS medium fortified with IBA at a concentration of (2.0 mg/l) within 90 days. These in vitro raised shoots with well developed roots were successfully acclimatized in vermicompost with 80% survival rate. In addition to in vitro shooting, ex vitro regeneration of shoots from in vitro raised rhizomes was also achieved in vermicompost within 49 days.

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Section: B Multiple Shoot Regeneration From Seed Explantsupporting

confidence: 92%

In vitro Propagation of Polygonatum verticillatum All. A Threatened Medicinal Herb through Seed Explant

Qadir¹,

Singh²,

Kour³

et al. 2020

JSR

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“…Callus induction started late after 3-4 weeks of culture in this study. Likewise, late callus induction from seed explant has been reported by Aasim et al [25] in hairy vetch and Javed et al [26] in Brassica napus. It was also noted that growth variants in the culture medium were more supportive for shoot induction rather than callus induction.…”

Section: Discussionmentioning

confidence: 57%

“…The suppressive effects of TDZ on shoot length might be consistent with its high cytokinin activity [37]. On the other hand, addition of IBA helped to overcome the negative effects of cytokinins; a has been reported in hairy vetch [25], fenugreek [38] and narbon vetch [39]. …”

Section: Discussionmentioning

confidence: 86%

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Adventitious Shoot Regeneration from Leaf Explant of Dwarf Hygro (Hygrophila polysperma (Roxb.) T. Anderson)

Karataş

Aasım

Çınar

et al. 2013

The Scientific World Journal

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Dwarf hygro (Hygrophila polysperma) is an ornamental aquatic plant that changes its leaf colours to pinkish in high light. It is listed as a medicinal plant in medicinal plant lists of Indian states of West Bengal and Karnataka. It is also used as a screening tool for toxicities and a bioindicator to detect and control algae. The study reported in vitro adventitious shoot regeneration from leaf explants cultured on MS medium containing 0.10–1.60 mg/L Kin/TDZ with or without 0.10 mg/L IBA and 500 mg/L Amoklavin to eradicate endogenic bacterial contamination. Direct adventitious shoot regeneration started within one week from both culture mediums followed by late callus induction which was more prominent on TDZ containing media compared to Kin containing media. Addition of 0.10 mg/L IBA with both Kin and TDZ increased shoot regeneration frequency, mean number of shoots per explant, and mean shoot length. Maximum number of 16.33 and 20.55 shoots per explant was obtained on MS medium containing 0.80 + 0.10 mg/L Kin-IBA and 0.10 + 0.10 mg/L TDZ-IBA, respectively. Regenerated shoots were rooted on MS medium containing 0.20–1.00 mg/L IBA followed by successfull acclimatization in aquariums. Regenerated plantlets were also tested in jars containing distilled water that showed the pH 6–9 for the best plant growth and development.

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“…Multiple axillary shoot regeneration from mature seed explants has been documented in hairy vetch (Aasim, Şahin Demirbağ, Khawar, Kendir, & Özcan, 2011) and related species of Vicia (Sancak, Mirici, & Özcan, 2000; Selva, Stouffs, & Briquet, 1989). However, no report of root generation from leaf or stem tissue has been published, to our knowledge, at this time.…”

Section: Introductionmentioning

confidence: 99%

Root and axillary shoot development of hairy vetch stem cuttings and cessation of flower development under a short photoperiod

2020

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Clonal propagation is a valuable tool for plant breeders to maintain heterozygous genotypes in crop species that are not amenable to self‐pollination or apomixis. An example is the drought‐tolerant forage and N‐fixing cover crop hairy vetch (Vicia villosa Roth), which primarily outcrosses and is developed into composite cultivars by plant breeders. The ability to obtain clones of desirable genotypes in breeding nurseries would enable the maintenance of heterozygous genotypes for genetic study or the ability to reconstitute a true synthetic cultivar. We developed an efficient method of clonal propagation from stem cuttings to maintain genotypes in a vegetative state for a minimum of 2 yr, even when obtained from flowering parental plants. The long‐term viability of such clones is unknown, however. We determined that hairy vetch, which flowers under long days, exhibits a form of partial flowering where vegetative meristems are maintained during conditions that induce flowering. Clones obtained from flowering parents would resume reproductive growth and eventually senesce if kept in inductive conditions. However, if clones were grown under a continuous 6‐h photoperiod, reproductive development would cease and vegetative growth would resume indefinitely.

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Direct axillary shoot regeneration from the mature seed explant of the hairy vetch (Vicia villosa Roth)

Cited by 11 publications

References 6 publications

In vitro Propagation of Polygonatum verticillatum All. A Threatened Medicinal Herb through Seed Explant

In vitro Propagation of Polygonatum verticillatum All. A Threatened Medicinal Herb through Seed Explant

Adventitious Shoot Regeneration from Leaf Explant of Dwarf Hygro (Hygrophila polysperma (Roxb.) T. Anderson)

Root and axillary shoot development of hairy vetch stem cuttings and cessation of flower development under a short photoperiod

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