Direct comparison of Altona-SARS-CoV-2 dual target RT-qPCR Assay with commercial LAMP Assay using throat washes in health care staff testing

Wanney, Johannes; Lüsebrink, Jessica; Spölgen, Gina; Demuth, Sabrina; Schildgen, Verena; Schildgen, Oliver

doi:10.1016/j.jcvp.2022.100088

Cited by 3 publications

(2 citation statements)

References 20 publications

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“…For PCR testing, oro-nasopharyngeal swabs (eSwab® by Copan) or pharynx gargle samples were used [ 13 ]. In most cases, HEs were tested in our in-house COVID-19 test centres by trained medical staff.…”

Section: Methodsmentioning

confidence: 99%

Long-term symptoms after SARS-CoV-2 infection in a cohort of hospital employees: duration and predictive factors

Gruber,

Montilva Ludewig,

Weßels

et al. 2024

BMC Infect Dis

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Purpose To evaluate the frequency, duration and patterns of long-term coronavirus disease 2019 (COVID-19) symptoms and to analyse risk factors for long-lasting COVID-19 sequelae among a cohort of hospital employees (HEs). Methods We conducted a survey regarding persistent COVID-19 related symptoms with all HEs from three medical centres in Cologne, Germany, who were tested SARS-CoV-2 PCR positive from March 2020 until May 2021. Duration of symptoms and possible risk factors for protracted COVID-19 course were analysed. Results Of 221 included HEs, a number of 104 HEs (47.1%) reported at least one persisting symptom for more than 90 days after initial SARS-CoV-2 detection. Each one cycle higher initial Ct value significantly increased the chances of overcoming symptoms (odds ratio [OR] 1.05; 95% confidence interval (95%CI) 1.01–1.09; p = 0.019). The occurrence of breathlessness within the first ten days (OR 7.89; 95%CI 1.87–41.43; p = 0.008), an initial Ct value under 30 (OR 3.36; 95%CI 1.22–9.94; p = 0.022) as well as the occurrence of anosmia or ageusia within the first ten days (OR 3.01; 95%CI 1.10–8.84; p = 0.037) showed a statistically significant association with increased odds of illness duration over 90 days. Conclusion About half of the HEs suffered from long lasting symptoms over 90 days after almost entirely mild acute COVID-19. Predictive factors could possibly be used for early treatment to prevent development of long-term symptoms after COVID-19 in future.

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Section: Methodsmentioning

confidence: 99%

Long-term symptoms after SARS-CoV-2 infection in a cohort of hospital employees: duration and predictive factors

Gruber,

Montilva Ludewig,

Weßels

et al. 2024

BMC Infect Dis

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“…This test is suitable for cost-effective, mass testing (Chaouch, 2021). On the other hand, it is prone to false-negative results if a person has a low viral load, and it also shows less sensitivity compared to the PCR-based methods described below (Pu et al, 2022;Wanney et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

Sensitive SARS-CoV-2 detection, air travel Covid-19 testing, variant determination and fast direct PCR detection, using ddPCR and RT-qPCR methods

Burjanivova¹,

Lukacova²,

Lucansky³

et al. 2023

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Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) monitoring in air traffic is important in the prevention of the virus spreading from abroad. The gold standard for SARS-CoV-2 detection is RT-qPCR; however, for early and low viral load detection, a much more sensitive method, such as droplet digital PCR (ddPCR), is required. Our first step was to developed both, ddPCR and RT-qPCR methods, for sensitive SARS-CoV-2 detection. Analysis of ten swab/saliva samples of five Covid-19 patients in different stages of disease showed positivity in 6/10 samples with RT-qPCR and 9/10 with ddPCR. We also used our RT-qPCR method for SARS-CoV-2 detection without the need of RNA extraction, obtaining results in 90-120 minutes. We analyzed 116 self-collected saliva samples from passengers and airport staff arriving from abroad. All samples were negative by RT-qPCR, while 1 was positive, using ddPCR. Lastly, we developed ddPCR assays for SARS-CoV-2 variants identification (alpha, beta, gamma, delta/kappa) that are more economically advantageous when compared to NGS. Our findings demonstrated that saliva samples can be stored at ambient temperature, as we did not observe any significant difference between a fresh sample and the same sample after 24 hours (p = 0.23), hence, saliva collection is the optimal route for sampling airplane passengers. Our results also showed that droplet digital PCR is a more suitable method for detecting virus from saliva, compared to RT-qPCR.

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Laboratory-based molecular test alternatives to RT-PCR for the diagnosis of SARS-CoV-2 infection

Arevalo-Rodriguez,

Mateos-Haro,

Dinnes

et al. 2024

Cochrane Database of Systematic Reviews

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Direct comparison of Altona-SARS-CoV-2 dual target RT-qPCR Assay with commercial LAMP Assay using throat washes in health care staff testing

Cited by 3 publications

References 20 publications

Long-term symptoms after SARS-CoV-2 infection in a cohort of hospital employees: duration and predictive factors

Long-term symptoms after SARS-CoV-2 infection in a cohort of hospital employees: duration and predictive factors

Sensitive SARS-CoV-2 detection, air travel Covid-19 testing, variant determination and fast direct PCR detection, using ddPCR and RT-qPCR methods

Laboratory-based molecular test alternatives to RT-PCR for the diagnosis of SARS-CoV-2 infection

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