Direct comparison of clinical diagnostic sensitivity of saliva from buccal swabs versus combined oro-/nasopharyngeal swabs in the detection of SARS-CoV-2 B.1.1.529 Omicron

Puyskens, Andreas; Michel, Janine; Stoliaroff-Pépin, Anna; Bayram, Fatimanur; Sesver, Akin; Wichmann, Ole; Harder, Thomas; Schaade, Lars; Nitsche, Andreas; Peine, Michael

doi:10.1016/j.jcv.2023.105496

Cited by 4 publications

(3 citation statements)

References 16 publications

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“…In our patients, repeated nasopharyngeal swabs were negative for SARS-CoV2 RT-PCR, but these negative results on nasopharyngeal swabs are clearly insufficient to rule out COVID-19 diagnosis [2]. We strongly recommend the use of lower respiratory tract specimen testing for the direct detection of SARS-CoV-2 infection in immunosuppressed patients, in case of clinical and/or radiological suspicion [11]. Morandi et al evaluated the additional value of lower respiratory tract sampling in the diagnosis of COVID-19 in patients with clinically suspected SARS-CoV-2 infection with available chest CT scan and at least two negative nasopharyngeal swab RT-PCR tests for SARS-CoV-2 [12].…”

Section: Discussionmentioning

confidence: 65%

Prolonged SARS-CoV-2 Infection in Patients Receiving Anti-CD20 Monoclonal Antibodies: A Diagnostic Challenged by Negative Nasopharyngeal RT-PCR and Successful Treatment with COVID-19 High-Titer Convalescent Plasma

Da Silva,

Klopfenstein,

Gendrin

et al. 2023

Viruses

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We highlighted in this current paper similar prolonged respiratory presentation with COVID-19 pneumonia in four severely immunocompromised patients currently being treated with anti-CD20 monoclonal antibodies (mAbs), such as ocrelizumab and rituximab, for multiple sclerosis or rheumatoid polyarthritis. Real-time reverse transcription-polymerase chain reaction on a nasopharyngeal swab specimen was negative in all patients. SARS-CoV-2 infection was confirmed from bronchoalveolar lavage fluid. A high titer of post-vaccine COVID-19 convalescent plasma was administered with complete recovery in all patients.

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Section: Discussionmentioning

confidence: 65%

Prolonged SARS-CoV-2 Infection in Patients Receiving Anti-CD20 Monoclonal Antibodies: A Diagnostic Challenged by Negative Nasopharyngeal RT-PCR and Successful Treatment with COVID-19 High-Titer Convalescent Plasma

Da Silva,

Klopfenstein,

Gendrin

et al. 2023

Viruses

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“…If buccal swabs or oral specimens are used for SARS-CoV-2 testing, it is recommended to collect them fasting in the morning before food intake, drinking, or brushing teeth, to increase the overall sensitivity. Importantly, the positivity rate would further decrease when other, less sensitive test methods such as rapid antigen tests are used [ 38 ], and when the patient is not instructed correctly.…”

Section: Discussionmentioning

confidence: 99%

Impact of Swabbing Location, Self-Swabbing, and Food Intake on SARS-CoV-2 RNA Detection

Dräger,

Bruni,

Bernasconi

et al. 2024

Microorganisms

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This study compared SARS-CoV-2 RNA loads at different anatomical sites, and the impact of self-swabbing and food intake. Adult symptomatic patients with SARS-CoV-2 or non-SARS-CoV-2 respiratory tract infection were included between 2021 and 2022. Patients performed a nasal and buccal swab before a professionally collected nasopharyngeal/oropharyngeal swab (NOPS). Buccal swabs were collected fasting and after breakfast in a subgroup of patients. SARS-CoV-2 RNA loads were determined by nucleic acid testing. Swabbing convenience was evaluated using a survey. The median age of 199 patients was 54 years (interquartile range 38−68); 42% were female and 52% tested positive for SARS-CoV-2. The majority of patients (70%) were hospitalized. The mean SARS-CoV-2 RNA load was 6.6 log10 copies/mL (standard deviation (SD), ±1.5), 5.6 log10 copies/mL (SD ± 1.9), and 3.4 log10 copies/mL (SD ± 1.9) in the professionally collected NOPS, and self-collected nasal and buccal swabs, respectively (p < 0.0001). Sensitivity was 96.1% (95% CI 90.4−98.9) and 75.3% (95% CI 63.9−81.8) for the nasal and buccal swabs, respectively. After food intake, SARS-CoV-2 RNA load decreased (p = 0.0006). Buccal swabbing was the preferred sampling procedure for the patients. In conclusion, NOPS yielded the highest SARS-CoV-2 RNA loads. Nasal self-swabbing emerged as a reliable alternative in contrast to buccal swabs. If buccal swabs are used, they should be performed before food intake.

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“…To overcome these constraints, alternative nucleic acid amplification methodologies, such as reverse transcription loop-mediated isothermal amplification (RT-LAMP) and microarray technologies, can be employed to devise cost-effective, expeditious, and efficient detection approaches. 14,15 On the other hand, serological assays, including multiplex immunoassays (MIA), enzyme-linked immunoassays (ELISA), and chemiluminescent immunoassays (CLIAs), for antibody detection have gained immense attention in asymptomatic patients as well as post-infection. 16…”

Section: Introduction: a Demi-decade In Combatting The Sars-cov-2 Out...mentioning

confidence: 99%

Engineered two-dimensional nanomaterials based diagnostics integrated with internet of medical things (IoMT) for COVID-19

Sadique,

Yadav,

Khan

et al. 2024

Chem. Soc. Rev.

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Direct comparison of clinical diagnostic sensitivity of saliva from buccal swabs versus combined oro-/nasopharyngeal swabs in the detection of SARS-CoV-2 B.1.1.529 Omicron

Cited by 4 publications

References 16 publications

Prolonged SARS-CoV-2 Infection in Patients Receiving Anti-CD20 Monoclonal Antibodies: A Diagnostic Challenged by Negative Nasopharyngeal RT-PCR and Successful Treatment with COVID-19 High-Titer Convalescent Plasma

Prolonged SARS-CoV-2 Infection in Patients Receiving Anti-CD20 Monoclonal Antibodies: A Diagnostic Challenged by Negative Nasopharyngeal RT-PCR and Successful Treatment with COVID-19 High-Titer Convalescent Plasma

Impact of Swabbing Location, Self-Swabbing, and Food Intake on SARS-CoV-2 RNA Detection

Engineered two-dimensional nanomaterials based diagnostics integrated with internet of medical things (IoMT) for COVID-19

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