Direct conversion of starch to L(+) lactic acid by amylase producing Lactobacillus amylophilus GV6

Abstract

Lactobacillus amylophilus strain GV6, isolated from corn starch processing industrial wastes, was amylolytic and produced 0.96 g L(+) lactic acid per gram of soluble starch. The optimum temperature and pH for growth and L(+) lactic acid production were 37°C and 6.5, respectively. At low substrate concentrations, the lactic acid production on corn starch was almost similar to soluble starch. The strain is fermenting various naturally available starches directly to lactic acid. The total amylase activity of the strain is 0.59 U/ml/min. The strain produced 49 and 76.2 g/l L(+) lactic acid from 60 g/l corn starch and 90 g/l soluble starch, respectively. This is the highest L(+) lactic acid among the wild strains of L. amylophilus reported so far.

Inoculated fermented corn as dietary performance and health enhancer in pigs: literature and in vitro study

“…Fermentation of corn starch has been initiated before (Vishnu et al 2002) by inoculation with Lactobacillus amylophilus GV6, a strain with a starch to lactic acid conversion efficiency of more than 80% when using crude starchy substrates. Lactobacillus amylophilus GV6, is an amylolytic lactic acid producing organism, isolated from cornstarch processing industrial wastes using MRS medium.…”

“…A corn specific Lactobacillus amylophilus GV6 (Vishnu et al 2002) may be used in combination with one or more pig specific probiotics like Lactobacillus acidophilus, Lactobacillus salivarius, Lactobacillus reuteri, Lactobacillus plantarum and Lactobacillus johnsonii depending on the availability of these strains. Commercially available pig probiotics may be used as alternative source of inoculation, if no restrictions to the use apply.…”

Application of Amylolytic Lactobacillus fermentum 04BBA19 in Fermentation for Simultaneous Production of Thermostable Alpha-Amylase and Lactic Acid

“…Owing to the ability of their α-amylases to partially hydrolyze raw starch, ALAB can ferment different types of amylaceous raw material, such as corn [17], potato [18], or cassava [19] and different starchy substrates [20,21,8]. Amylolytic LAB utilize starchy biomass and convert it into lactic acid in a single step fermentation.…”

“…The bioconversion of carbohydrate materials to lactic acid can be made much more effective by coupling the enzymatic hydrolysis of carbohydrate substrates and microbial fermentation of the derived glucose into a single step. This has been successfully employed for lactic acid production from raw starch materials with many representative bacteria including Lactobacillus and Lactococcus species [23,20,24,21].…”

Production of Lactic Acid by Lactic Acid Bacteria Isolated from Shellfish

“…젖산 중합체 (lactic acid polymer)는 생분해성, 열가소성과 고강도성의 장점을 가지고 있으며, 이러한 장점은 플라스틱 분야의 사용에 있어서 잠재 적인 시장 가능성을 가지게 된다 [3]. 젖산발효에 있어서 생물공학적 생산의 가장 큰 장점은 당 밀, 탄수화물, 셀룰로오스와 같은 발효공정에서의 원부자재 의 가격이 매우 저렴하다는 데 있다 [4,5]. 젖산은 화학적 전 환기술로 중합 중간체인 락타이드 (lactide)나 아크릴산 (acrylic acid) 등의 유용 화학물질로 변환된다.…”