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Lactobacillus amylophilus strain GV6, isolated from corn starch processing industrial wastes, was amylolytic and produced 0.96 g L(+) lactic acid per gram of soluble starch. The optimum temperature and pH for growth and L(+) lactic acid production were 37°C and 6.5, respectively. At low substrate concentrations, the lactic acid production on corn starch was almost similar to soluble starch. The strain is fermenting various naturally available starches directly to lactic acid. The total amylase activity of the strain is 0.59 U/ml/min. The strain produced 49 and 76.2 g/l L(+) lactic acid from 60 g/l corn starch and 90 g/l soluble starch, respectively. This is the highest L(+) lactic acid among the wild strains of L. amylophilus reported so far.

Aims: Development of a simple, speci®c, rapid and inexpensive Dot-ELISA test for diagnosis of rotaviral antigen in stool samples. Methods and Results: Hyperimmune rabbit antisera raised against SA-11 (Simian Agent-11) strain was used as primary antibody. The secondary antibody conjugate used was the goat antirabbit IgG alkaline phosphatase, and BCIP/NBT solution was used as substrate. Faecal extracts were diluted 10-fold and used for the detection of rotavirus antigen. RNA-PAGE was performed to compare the speci®city and sensitivity of the diagnostic tests. Dot-ELISA positive samples were further con®rmed by Western blot analysis. Conclusions: This Dot-ELISA test could be used as an alternative method for diagnosing rotaviral samples in the ®eld. Signi®cance and Impact of the Study: The Dot-ELISA test is simple, speci®c, rapid and cost effective. It is suitable for identifying a large number of samples obtained from epidemiological studies and hence, reducing the death rate of rotavirus-infected patients.

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