Direct detection of circRNA in real samples using reverse transcription-rolling circle amplification

Liu, Yaqian; Zhang, Xiong; Liu, Min; Xu, Fa; Zhang, Qiuyang; Zhang, Yajun; Weng, Xiaocheng; Liu, Song-Mei; Du, Yuhao; Zhou, Xiang

doi:10.1016/j.aca.2019.12.027

Cited by 37 publications

(22 citation statements)

References 16 publications

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“…The third-generation highthroughput single-molecule sequencing technology has great advantages in the field of circular RNA research. Its long readlength eliminates the over-fragmentation characteristics of second-generation sequencing fundamentally, which brings hope about the detection of the full length of circRNA and Compared with the traditional circRNA detection methods, reverse transcription rolling circle amplification (RT-RCA) assay has the advantages of simple operation, low cost, and high sensitivity (103). It can even distinguish the circRNA from the linear RNA bearing the same sequence, which is superior to most methods such as qRT-PCR.…”

Section: Detection Of Circrnas In Body Fluidsmentioning

confidence: 99%

“…Compared with the traditional circRNA detection methods, reverse transcription rolling circle amplification (RT-RCA) assay has the advantages of simple operation, low cost, and high sensitivity ( 103 ). It can even distinguish the circRNA from the linear RNA bearing the same sequence, which is superior to most methods such as qRT-PCR.…”

Section: Detection Of Circrnas In Body Fluidsmentioning

confidence: 99%

“…One of the major concerns is that the methods for discovering and analyzing circRNAs are far from ideal, although enormous progress has been made in the past decades ( 127 , 128 ). Future research need to gauge the analytical performance of disparate circRNA analysis in clinical specimens, such as RNA-seq, circRNA microarray, RT-qPCR, ddPCR, and tCLN ( 103 , 128 ). Attention must be paid to standardizing in circRNA discovery and analysis when estimating the sensitivity and specificity ( 129 ).…”

Section: Challenges and Perspectivesmentioning

confidence: 99%

See 2 more Smart Citations

The Value of Circulating Circular RNA in Cancer Diagnosis, Monitoring, Prognosis, and Guiding Treatment

Zhang

Wang

et al. 2021

Front. Oncol.

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Liquid biopsy includes non-invasive analysis of circulating tumor-derived substances. It is a novel, innovative cancer screening tool that overcomes the limitations of current invasive tissue examinations in precision oncology. Circular RNA (circRNA) is a recent, novel, and attractive liquid biomarker showing stability, abundance, and high specificity in various diseases, especially in human cancers. This review focused on the emerging potential of human circRNA in body fluids as the liquid biopsy biomarkers for cancers and the methods used to detect the circRNA expression and summarized the construction of circRNA biomarkers in body fluids for treating human cancers and their limitations before they become part of routine clinical medicine. Furthermore, the future opportunities and challenges of translating circRNAs in liquid biopsy into clinical practices were explored.

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Section: Detection Of Circrnas In Body Fluidsmentioning

confidence: 99%

Section: Detection Of Circrnas In Body Fluidsmentioning

confidence: 99%

Section: Challenges and Perspectivesmentioning

confidence: 99%

See 1 more Smart Citation

The Value of Circulating Circular RNA in Cancer Diagnosis, Monitoring, Prognosis, and Guiding Treatment

Zhang

Wang

et al. 2021

Front. Oncol.

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“…C Schematic diagram for sensitive detection of lncRNA based on DSN and APE1 enzyme mediated triple cycle amplification [ 78 ]. D Schematic diagram for sensitive detection of circRNA based on RCA [ 84 ] …”

Section: Application Of Csa For Fluorescent Biosensorsmentioning

confidence: 99%

“…As an important biomarker for disease diagnosis, it is necessary to develop sensitive detection methods for circRNA detection. Liu et al proposed a reverse transcription-RCA (RT-RCA) method for sensitively detecting circRNA [ 84 ] (Fig. 6 D).…”

Section: Application Of Csa For Fluorescent Biosensorsmentioning

confidence: 99%

Recent advances of fluorescent biosensors based on cyclic signal amplification technology in biomedical detection

Fan

Chen

et al. 2021

J Nanobiotechnol

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The cyclic signal amplification technology has been widely applied for the ultrasensitive detection of many important biomolecules, such as nucleic acids, proteins, enzymes, adenosine triphosphate (ATP), metal ions, exosome, etc. Due to their low content in the complex biological samples, traditional detection methods are insufficient to satisfy the requirements for monitoring those biomolecules. Therefore, effective and sensitive biosensors based on cyclic signal amplification technology are of great significance for the quick and simple diagnosis and treatment of diseases. Fluorescent biosensor based on cyclic signal amplification technology has become a research hotspot due to its simple operation, low cost, short time, high sensitivity and high specificity. This paper introduces several cyclic amplification methods, such as rolling circle amplification (RCA), strand displacement reactions (SDR) and enzyme-assisted amplification (EAA), and summarizes the research progress of using this technology in the detection of different biomolecules in recent years, in order to provide help for the research of more efficient and sensitive detection methods. Graphical Abstract

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Circular RNAs in human diseases

Wang,

Zhang,

Yang

et al. 2024

MedComm

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Circular RNAs (circRNAs) are a unique class of RNA molecules formed through back‐splicing rather than linear splicing. As an emerging field in molecular biology, circRNAs have garnered significant attention due to their distinct structure and potential functional implications. A comprehensive understanding of circRNAs’ functions and potential clinical applications remains elusive despite accumulating evidence of their involvement in disease pathogenesis. Recent research highlights their significant roles in various human diseases, but comprehensive reviews on their functions and applications remain scarce. This review provides an in‐depth examination of circRNAs, focusing first on their involvement in non‐neoplastic diseases such as respiratory, endocrine, metabolic, musculoskeletal, cardiovascular, and renal disorders. We then explore their roles in tumors, with particular emphasis on exosomal circular RNAs, which are crucial for cancer initiation, progression, and resistance to treatment. By detailing their biogenesis, functions, and impact on disease mechanisms, this review underscores the potential of circRNAs as diagnostic biomarkers and therapeutic targets. The review not only enhances our understanding of circRNAs’ roles in specific diseases and tumor types but also highlights their potential as novel diagnostic and therapeutic tools, thereby paving the way for future clinical investigations and potential therapeutic interventions.

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Direct detection of circRNA in real samples using reverse transcription-rolling circle amplification

Cited by 37 publications

References 16 publications

The Value of Circulating Circular RNA in Cancer Diagnosis, Monitoring, Prognosis, and Guiding Treatment

The Value of Circulating Circular RNA in Cancer Diagnosis, Monitoring, Prognosis, and Guiding Treatment

Recent advances of fluorescent biosensors based on cyclic signal amplification technology in biomedical detection

Circular RNAs in human diseases

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