Direct Detection of Lysozyme in Viscous Raw Hen Egg White Binding to Sodium Dodecyl Sulfonate by Reactive Wooden-tip Electrospray Ionization Mass Spectrometry

Wu, Lin; Yao, Ya-Nan; Yuan, Zi-Cheng; Di, Dandan; Li, Lei; Hu, Bin

doi:10.2116/analsci.19p288

Cited by 9 publications

(7 citation statements)

References 41 publications

(54 reference statements)

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“…Particularly, free heme at m/z 616.18 was detected; the heme detection confirmed that Hb is totally denatured under the organic solvent. Low-background-noise (Figure 1c) solvent is generated a from blank wooden material that is similar to a previous work using wooden-tip ESI [32][33][34].…”

Section: Direct Analysis Of Whole Blood Samplesmentioning

confidence: 84%

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Wooden-Tip Electrospray Mass Spectrometry Characterization of Human Hemoglobin in Whole Blood Sample for Thalassemia Screening: A Pilot Study

Huang

Zou

et al. 2022

Molecules

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Traditional analytical methods for thalassemia screening are needed to process complicated and time-consuming sample pretreatment. In recent decades, ambient mass spectrometry (MS) approaches have been proven to be an effective analytical strategy for direct sample analysis. In this work, we applied ambient MS with wooden-tip electrospray ionization (WT-ESI) for the direct analysis of raw human blood samples that were pre-identified by gene detection. A total of 319 whole blood samples were investigated in this work, including 100 α-thalassemia carriers, 67 β-thalassemia carriers, and 152 control healthy samples. Only one microliter of raw blood sample was directly loaded onto the surface of the wooden tip, and then five microliters of organic solvent and a high voltage of +3.0 kV were applied onto the wooden tip to generate spray ionization. Multiply charged ions of human hemoglobin (Hb) were directly observed by WT-ESI-MS from raw blood samples. The signal ratios of Hb chains were used to characterize two main types of thalassemia (α and β types) and healthy control blood samples. Our results suggested that the ratios of charged ions to Hb chains being at +13 would be an indicator for β-thalassemia screening.

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Section: Direct Analysis Of Whole Blood Samplesmentioning

confidence: 84%

“…It is reported that different protein structures can be recognized by ambient MS [31]. In previous studies [32,33], WT-ESI-MS has been successfully used for protein analysis. Furthermore, the mobility of proteins in wooden tips could be characterized under ESI conditions [34].…”

Section: Introductionmentioning

confidence: 99%

Wooden-Tip Electrospray Mass Spectrometry Characterization of Human Hemoglobin in Whole Blood Sample for Thalassemia Screening: A Pilot Study

Huang

Zou

et al. 2022

Molecules

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“…As shown in Figure 2(F), the wooden tip was directly mounted on a nanoESI device of an Orbitrap mass spectrometer (Q‐Exactive, Thermo Fisher Scientific, Bremen, Germany) as described previously 23,24 . Briefly, the distance between the wooden tip‐end and MS inlet was ca.…”

Section: Methodsmentioning

confidence: 99%

Investigating distributions and changes of alkaloids in living Catharanthus roseus under low‐phosphorus stress using wooden‐tip electrospray ionisation mass spectrometry

Yao

2020

Phytochemical Analysis

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Introduction: Vinca alkaloids are important sources for producing anticancer drugs from Catharanthus roseus. The phosphorus of soil is one of crucial factors for planting C. roseus. Objectives: We aim to develop an in vivo sampling technique coupled with direct mass spectrometry with wooden tip for investigating distributions and changes of alkaloids in flowers, leaves, stems, veins and roots of living C. roseus under lowphosphorus stress. Materials and Methods: Living C. roseus were prepared under low-phosphorus stress (n = 10) and control conditions (n = 10). Wooden-tip electrospray ionisation mass spectrometry and conventional liquid chromatography-mass spectrometry were applied to analyse living C. roseus and extracts of C. roseus, respectively. Results: Distributions and changes of serpentine, vindoline, catharanthine, and anhydrovinblastine in living C. roseus under low-phosphorus stress and control conditions were successfully obtained. Conclusion: Compared to control soil conditions, low-phosphorus soil was found to induce C. roseus to generate more serpentine but less catharanthine and vindoline in leaves, veins, stems and roots, and to generate more anhydrovinblastine in flowers, leaves, stems and roots. Overall, our results showed a simple, rapid, and effective method for in vivo sampling and direct analysis of living plants.

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“…In recent years, native mass spectrometry has been used to characterise allergens and their interactions, with one study using novel wooden tips to analyse egg white. 41,42 In this study we apply an adapted version of this methodology to the analysis of protein rich natural products. We deliberately chose examples that are sources of commercially available "standard" proteins, as well as widely consumed food substances.…”

Section: Introductionmentioning

confidence: 99%

Using Native Mass Spectrometry to Analyse Proteins Directly from Food

France

Bramonti

Vickers

et al. 2023

Preprint

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Globally, food is a multi-trillion-pound industry for which proteomic analysis represents a key tool in ensuring that consumer health and rights are maintained. Here we use native mass spectrometry methodology to analyse a series of natural food products of varying complexity, namely: cow milk (liquid); chicken egg white (viscous liquid) and jack bean meal (solid). Our approach permits rapid detection (~5-30 mins) and unambiguous identification of the majority (>80%) of proteins present within milk and egg white, which are foodstuffs that between them comprise two of the most prominent sources of allergenic proteins within the food industry. Furthermore, we show that this method also enables the retention of bioactive protein complexes directly from natural sources, exemplified by the detection of three multimeric states (monomer, dimer and tetramer) of concanavalin A, naturally found in jack beans (Canavalia ensiformis). As such, we propose that native mass spectrometry methods can augment the current bottom up proteomic toolkit employed within food analyses and may prove useful for fast detection and high accuracy identification of suspected proteinaceous allergens/adulterants within sufficiently noncomplex food substances.

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Direct Detection of Lysozyme in Viscous Raw Hen Egg White Binding to Sodium Dodecyl Sulfonate by Reactive Wooden-tip Electrospray Ionization Mass Spectrometry

Cited by 9 publications

References 41 publications

Wooden-Tip Electrospray Mass Spectrometry Characterization of Human Hemoglobin in Whole Blood Sample for Thalassemia Screening: A Pilot Study

Wooden-Tip Electrospray Mass Spectrometry Characterization of Human Hemoglobin in Whole Blood Sample for Thalassemia Screening: A Pilot Study

Investigating distributions and changes of alkaloids in living Catharanthus roseus under low‐phosphorus stress using wooden‐tip electrospray ionisation mass spectrometry

Using Native Mass Spectrometry to Analyse Proteins Directly from Food

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