Direct Detection of the Biological Toxin in Acidic Environment by Electrochemical Impedimetric Immunosensor

Chai, Changhoon; Lee, Jooyoung; Takhistov, Paul

doi:10.3390/s101211414

Cited by 24 publications

(6 citation statements)

References 17 publications

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“…The sensitivity of EIBs for the detection and measurement of food hazards is comparable to or better than that of other biosensors and traditional methods (Ahmed et al, 2014;Bahadır and Sezgintürk, 2016;Malvano et al, 2019). EIBs can detect and measure food hazards in less than 1 h (Ahmed et al, 2014;Chai et al, 2010;Malvano et al, 2019). In combination with the Internet of Things (IoT), EIBs may evolve into real-time food safety monitoring systems (Durresi, 2016).…”

Section: Introductionmentioning

confidence: 99%

Electrochemical impedimetric biosensors for food safety

Chai

2020

Food Sci Biotechnol

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Electrochemical impedimetric biosensors (EIBs) have a simple structure and can be used to rapidly and sensitively detect and measure hazards in food. EIBs detect and measure target molecules by transducing biochemical reactions on their surface to electrical signal outputs responding to a sinusoidal electrical signal input. Due to their structural simplicity and analytical sensitivity, EIBs are regarded as the most potent method of food hazard monitoring that can be implemented in the food supply chain. This paper discusses the theoretical background, structure, and construction of EIB and its applications in food safety. Keywords Electrochemical impedance spectroscopy Á Biosensor Á Food safety Á Pathogen Á Mycotoxin max The maximum value of Z re R s The electrolyte resistance C dl The double-layer capacitance R ct The charge-transfer resistance e The dielectric constant d The thickness of the electrical double layer

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Section: Introductionmentioning

confidence: 99%

Electrochemical impedimetric biosensors for food safety

Chai

2020

Food Sci Biotechnol

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“…Next, the compactness of the self-assembled monolayer was assessed. According to the literature, the higher the degree of APTES assembled on the surface of the silicon wafer, the surface will be gradually alkylated and become more hydrophobic due to the influence of the carbon chain on the molecular structure . It showed that when the self-assembly time is 30 min, the degree of surface assembly will reach the densest, and the water contact angle has a maximum value of 85.95 ± 2.11° at this time.…”

Section: Resultsmentioning

confidence: 99%

Zwitterionic Surface Modification of Aldehydated Sulfobetaine Copolymers for the Formation of Bioinert Interfaces

Chou

2023

ACS Appl. Polym. Mater.

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This research successfully developed a zwitterionic copolymer, poly(2-oxoethyl methacrylate-co-sulfobetaine methacrylate) poly(OxMA-co-SBMA), in which the OxMA segments have an aldehyde functional group that can undergo a nucleophilic addition reaction with an amine group to generate a Schiff base. At the same time, SBMA units are employed as antifouling groups. This copolymer is grafted via a graf t-to process to the surface of an amine-based substrate to form chemical bonds by dip-coating technology and achieves antifouling properties. The synthesized monomer and copolymers are characterized by Fourier transform infrared (FTIR) spectroscopy and NMR to confirm their chemical structure. Notably, dip-coating technology uses a simple "graf t-to" immersion process to achieve nonfouling and antiprotein properties. Compared to the previously reported ″graft-f rom″ technology, dip-coating has the advantage of a fast and simple procedure and is easy to scale up. The poly(OxMA-co-SBMA) copolymer was grafted onto the amino substrate surface by controlling the dip-coating conditions of different grafting temperatures, grafting time, concentrations of copolymer solution, and copolymer molar ratios. The prepared poly(OxMA-co-SBMA) with an optimum pOxMA/ pSBMA molar ratio of 1:4 and a molecular weight of 46.8 kDa exhibited an improvement of hydrophilicity and the best resistance to protein adsorption with over 90% reduction, as well as blood cell activation, tissue cell adhesion, and bacterial attachment on the zwitterionic copolymer-grafted surfaces. The results showed that the modified samples under the best grafting conditions could effectively reduce the biofouling of biomolecules. Meanwhile, it performed well in modified amine-based plastic materials, such as polyurethane tubes and polyamide bending fabrics, which are expected to have broader bioinert application potential in biomedical materials.

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“…An interesting example of an immunosensor using impedimetric detection is given in a 2010 study carried out by Chai et al (2010). In this work, an aluminum electrode was immunofunctionalized via covalent bonding onto SAM, and the authors were able to detect the presence of the biological toxin ricin in 20 min.…”

Section: Electrochemical Impedance Spectroscopymentioning

confidence: 99%

Biological Toxins and Bioterrorism

Gopalakrishnakone¹,

Balali-Mood²,

Llewellyn³

et al. 2015

Toxinology

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In recent years, the field of toxinology has expanded substantially. On the one hand it studies venomous animals, plants and micro organisms in detail to understand their mode of action on targets. While on the other, it explores the biochemical composition, genomics and proteomics of toxins and venoms to understand their three interaction with life forms (especially humans), development of antidotes and exploring their pharmacological potential. Therefore, toxinology has deep linkages with biochemistry, molecular biology, anatomy and pharmacology. In addition, there is a fast-developing applied subfield, clinical toxinology, which deals with understanding and managing medical effects of toxins on human body. Given the huge impact of toxin-based deaths globally, and the potential of venom in generation of drugs for so-far incurable diseases (for example, diabetes, chronic pain), the continued research and growth of the field is imminent. This has led to the growth of research in the area and the consequent scholarly output by way of publications in journals and books. Despite this ever-growing body of literature within biomedical sciences, there is still no all-inclusive reference work available that collects all of the important biochemical, biomedical and clinical insights relating to toxinology. Composed of 11 volumes, Toxinology provides comprehensive and authoritative coverage of the main areas in toxinology, from fundamental concepts to new developments and applications in the field. Each volume comprises a focused and carefully chosen collection of contributions from leading names in the subject.

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Direct Detection of the Biological Toxin in Acidic Environment by Electrochemical Impedimetric Immunosensor

Cited by 24 publications

References 17 publications

Electrochemical impedimetric biosensors for food safety

Electrochemical impedimetric biosensors for food safety

Zwitterionic Surface Modification of Aldehydated Sulfobetaine Copolymers for the Formation of Bioinert Interfaces

Biological Toxins and Bioterrorism

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