Direct detection of unamplified hepatoma upregulated protein RNA in urine using gold nanoparticles for bladder cancer diagnosis

Eissa, Sanaa; Shawky, Sherif M.; Matboli, Marwa; S.S., Mohamed,; Azzazy, Hassan M.E.

doi:10.1016/j.clinbiochem.2013.10.022

Cited by 33 publications

(20 citation statements)

References 26 publications

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“…Total RNA extraction was conducted using Promega SV-total RNA isolation system following manufacture instructions and each transcript was further purified by homemade magnetic nanoparticles as described previously (Eissa et al, 2014, Shawky et al, 2014), to enhance the purity of the extracted transcript.…”

Section: Methodsmentioning

confidence: 99%

Gold aggregating gold: A novel nanoparticle biosensor approach for the direct quantification of hepatitis C virus RNA in clinical samples

Shawky

Awad

Allam

et al. 2017

Biosensors and Bioelectronics

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The affordable and reliable detection of Hepatitis C Virus (HCV) RNA is a cornerstone in the management and control of infection, affecting approximately 3% of the global population. However, the existing technologies are expensive, labor intensive and time consuming, posing significant limitations to their wide-scale exploitation, particularly in economically deprived populations. Here, we utilized the unique optical and physicochemical properties of gold nanoparticles (AuNPs) to develop a novel assay platform shown to be rapid and robust in sensing and quantifying unamplified HCV RNA in clinical samples. The assay is based on inducing aggregation of citrate AuNPs decorated with a specific nucleic acid probe. Two types of cationic AuNPs, cysteamine and CTAB capped, were compared to achieve maximum assay performance. The technology is simple, rapid, cost effective and quantitative with 93.3% sensitivity, high specificity and detection limit of 4.57 IU/µl. Finally, our data suggest that RNA folding impact the aggregation behavior of the functionalized AuNPs, with broader applications in other nucleic acid detection technologies.

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Section: Methodsmentioning

confidence: 99%

Gold aggregating gold: A novel nanoparticle biosensor approach for the direct quantification of hepatitis C virus RNA in clinical samples

Shawky

Awad

Allam

et al. 2017

Biosensors and Bioelectronics

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“…This technology is the driving force behind the creation of nanomaterials for many emerging nanotechnology industries today. The applications of nanotechnology are far-reaching, with exposures occurring through industrial, commercial, and even medical applications (Hussain et al, 2013; Eissa et al, 2014). It has been predicted that the economic impact of nanotechnology will soon surpass the effect of the industrial revolution of the early 20 th Century (Nel et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Selective inhibitory effects of 50-nm gold nanoparticles on mouse macrophage and spleen cells

Kingston¹,

Pfau

Gilmer

et al. 2015

Journal of Immunotoxicology

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Nanoparticles (NP) are significant to multiple industrial processes, consumer products, and medical applications today. The health effects of many different types of NP, however, are largely unknown. The purpose of this study was to test the effects of 50-nm gold NP coated with poly-N-vinylpyrrolidone (PVP) on mouse macrophage and spleen cells with and without lipopolysaccharide (LPS), testing the hypothesis that the NP would modulate immune responses without being overtly toxic. Gold NP had no effect on macrophage viability, and in the absence of LPS, they had no effect on tumor necrosis factor (TNF)-α production as measured by ELISA. The presence of LPS significantly increased the release of TNFα from the macrophages above no-treatment controls, but increasing gold NP concentration led to decreasing release of TNFα. The reactive oxygen species (ROS) produced by exposed macrophages were also reduced compared to untreated controls both with and without LPS, suggesting some kind of oxygen radical scavenging. In splenocyte cultures, gold NP had no effect alone, but significantly reduced the release of interleukin (IL)-17 and TNFα triggered by LPS. These results suggest that the gold NP used here are not cytotoxic to immune cells at these concentrations, but may affect cellular responses to infection or inflammation by altering the balance of cytokines.

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“…Our research group used unmodified AuNPs assay for the direct detection of HURP unamplified RNA in clinical urine samples, which is an emerging biomarker for the early detection of schistosomal and non-schistosomal BC [82]. Also, we have incorporated functionalized magnetic nanoparticles for specific capturing of the HURP RNA from the total RNA extracted in urine samples.…”

Section: Five-year Viewmentioning

confidence: 99%

“…Mutant p53 PCR-SSCP followed by DNA sequencing [39] HURP Semiquantitative RT-PCR, nanoassay [50,82] Bladder tumor antigen (BTA)and telomerase…”

Section: -Genetic Urinary Biomarkers Related To Cell Cyclementioning

confidence: 99%

Urine biomarkers of schistosomiais and its associated bladder cancer

Eissa

Matboli

Shawky

et al. 2015

Expert Review of Anti-infective Therapy

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Schistosomiasis (SCH) is the second only to malaria among the parasitic diseases affecting humans regarding the prevalence of infection worldwide. In this nonsystematic review, we summarize the existing data on commercially available and promising investigational urine markers for the detection of SCH and its associated bladder cancer (BC). We searched PubMed, Scopus and Cochran without time limits. We reviewed the recent literatures on urine-based markers for SCH and its associated BC. Many studies identified several urine biomarkers of Schistosoma haematobium and Schistosoma mansoni worms and their associated BC using automated, inexpensive, quantitative assays in urine. These markers may aid in early detection of bladder carcinoma and have the potential to reduce the number of follow-up cystoscopy, thus reducing healthcare costs and patient discomfort, at the same time. Nevertheless, clinical evidence is insufficient to warrant the substitution of the cystoscopic follow-up scheme by any of the currently available urine marker tests.

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Direct detection of unamplified hepatoma upregulated protein RNA in urine using gold nanoparticles for bladder cancer diagnosis

Cited by 33 publications

References 26 publications

Gold aggregating gold: A novel nanoparticle biosensor approach for the direct quantification of hepatitis C virus RNA in clinical samples

Gold aggregating gold: A novel nanoparticle biosensor approach for the direct quantification of hepatitis C virus RNA in clinical samples

Selective inhibitory effects of 50-nm gold nanoparticles on mouse macrophage and spleen cells

Urine biomarkers of schistosomiais and its associated bladder cancer

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