Direct determination of poly(3-hydroxybutyrate) accumulated in bacteria by thermally assisted hydrolysis and methylation-gas chromatography in the presence of organic alkali

Abstract

Abstract:There is considerable interest in the development of simple methods for quantifying production of the biodegradable polyester poly(3-hydroxybutyrate) [P(3HB)] by bacteria. Cells of Cupriavidus necator were grown on agar medium containing different concentrations of glucose (10-25 g/L) as a sole carbon source. Trace amounts (100 ± 5 μg) of dried C. necator cells were directly subjected to thermally assisted hydrolysis and methylation-gas chromatography (THM-GC) in the presence of tetramethylammonium hydroxide (TMAH). On the resulting chromatograms, a series of characteristic peaks, attributed to the THM products from poly(3-hydroxybutylate) accumulated in the bacterial cells, were clearly observed without any interfering component. Based on the peak intensities, the contents of P(3HB) in C. necator were determined precisely and rapidly without using any cumbersome sample pretreatment. Furthermore, the values of the P(3HB) contents coincided overall with those obtained by the conventional method involving solvent extraction followed by gravimetric determination.

Recent Development of Polyhydroxyalkanoates (PHA)-Based Materials for Antibacterial Applications: A Review

“…Moreover, bioplastics offer better biodegradability and biocompatibility and have nontoxic behavior. 221,222 The first traditional applications of PHAs were the packaging materials and coating used in cosmetology and everyday articles, such as bottles, vials, and containers. PHA-based products were also found in household goods and disposable items, including utensils, hygiene products, and compostable bags.…”

“…Replacing petroleum-derived plastics with bioplastics like polyhydroxy­alkanoates (PHAs) has attracted attention due to their valuable properties, which are equivalent to conventional polymers. Moreover, bioplastics offer better biodegradability and biocompatibility and have nontoxic behavior. , The first traditional applications of PHAs were the packaging materials and coating used in cosmetology and everyday articles, such as bottles, vials, and containers. PHA-based products were also found in household goods and disposable items, including utensils, hygiene products, and compostable bags .…”

Dual application of black soldier fly (Hermetia illucens): Protein-rich animal feed and biological extraction agent for polyhydroxybutyrate

“…The PHB were quantify using methanolysis followed by gas chromatography (GC) analysis (Baidurah et al, 2016;Baidurah et al, 2018;Sen et al, 2019;Khok et al, 2020).…”

“…Polyhydroxybutyrate (PHB) and its co-polymer is a type of intracellular biodegradable polymer with a great potential as an alternative to petroleum-derived plastics due do its properties which are like conventional polymers and additionally, possess other important benefits such as biodegradability, biocompatibility and non-toxic characteristics (Baidurah et al, 2018;Sen et al, 2019). These unique properties of PHB and its co-polymer gave rise to diverse applications ranging from biodegradable packaging materials to biocompatible medical devices and tissue engineering materials (Chaijamrus and Udpuay, 2008;Bhagowati et al, 2015;Baidurah et al, 2018;Boey et al, 2021). The current PHB extraction method is by physicochemical and mechanical means (Kunasundari and Sudesh, 2011).…”

A Review of the Applications and Biodegradation of Polyhydroxyalkanoates and Poly(lactic acid) and Its Composites

“…Consequently, rather than decompose, these plastics tend to accumulate in landfills, oceans, and the natural environment. Replacement of petroleum-derived plastics with bioplastic such as polyhydroxyalkanoates (PHAs) and poly(lactic acid) (PLA) has attracted much attention due to their beneficial properties, which are similar to conventional polymers and, additionally, they possess other important benefits such as biodegradability, biocompatibility, and non-toxic behaviour [ 9 , 10 ]. These unique properties of biopolymers gave rise to diverse applications in industries ranging from biodegradable packaging materials to biocompatible medical devices and tissue engineering [ 11 , 12 ].…”