Direct Determination of Sulfacetamide Sodium by Derivative UV Spectrophotometry

Abstract

UV Derivative spectra were used for the direct quantitative determination of sulfacetamide sodium in distilled water. The quantification was accomplished using the integrated area under the peaks. The zero-order spectrum of sulfacetamide sodium shows an absorption band at λ = 258 nm, with an ε max = 14900 l. mol -1 . cm -1 , the determination limit was (0.55-25.4) µg/ml with R 2 = 0.9999 and RSD =1.25 %.The determination limits were (0.25-50.80) µg/ml for both first and second-order derivatives with R 2 = 0.9982, 0.9995 and RSD = 1.60 %, 2.40 % respectively. This indicates a more sensitive and accurate results as compared with the zero-order method. These methods were applied to the direct determination of the samacetamide eye drop.