No abstract

UV Derivative spectra were used for the direct quantitative determination of sulfacetamide sodium in distilled water. The quantification was accomplished using the integrated area under the peaks. The zero-order spectrum of sulfacetamide sodium shows an absorption band at λ = 258 nm, with an ε max = 14900 l. mol -1 . cm -1 , the determination limit was (0.55-25.4) µg/ml with R 2 = 0.9999 and RSD =1.25 %.The determination limits were (0.25-50.80) µg/ml for both first and second-order derivatives with R 2 = 0.9982, 0.9995 and RSD = 1.60 %, 2.40 % respectively. This indicates a more sensitive and accurate results as compared with the zero-order method. These methods were applied to the direct determination of the samacetamide eye drop.

No abstract

The UV fourth order derivative spectra were selected as the more reliable technique for the direct quantification of pure Tetracycline Hydrochloride in water, after the measurement of the zero, first, second, third and fourth order spectra, within the concentration range at (0.2885-144.27)µg/ml with R 2 =0.9998 and RSD=0.215 %. Bovine serum albumin was directly quantified by the fourth order UV derivative technique within the concentration range (6.7-2680) µg/ml with R 2 = 0.9998 and RSD=0.324 %. Appreciable interaction between Tetracycline hydrochloride and Bovine serum Albumin was found in accordance with the fraction coefficient and the apparent binding constant. The UV-fourth order derivative technique appears to be in a good accuracy and precise method for the direct quantification of Tetracycline Hydrochloride and Bovine Serum Albumin (BSA) in water.