Direct drug susceptibility testing of Mycobacterium tuberculosis using the proportional method: A multicenter study

Amini, Sirus; Hoffner, Sven; Torkaman, Mohammad; Hamzehloo, Gholamreza; Nasiri, Mohammad Javad; Salehi, Mahshid; Kashkooli, Golnar Sami; Shahraki, Mohammad Sadegh; Mohsenpoor, Maryam; Soleimanpour, Saman; Mir, Raha

doi:10.1016/j.jgar.2018.12.022

Cited by 11 publications

(6 citation statements)

References 17 publications

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“…The proportion method using Middlebrook 7H10 agar has been the "gold standard" method for several decades. 66…”

Section: Mtb Proportional Dst Methodsmentioning

confidence: 99%

“…There are over 170 known mycobacterial species, most related to the nontuberculous mycobacteria (NTM) group, with some belonging to the MTB complex (MTBc), sharing 99.9% genomic similarity. 66 Restriction endonuclease analysis and nucleic acid hybridization were employed for TB diagnosis. These approaches provided a strategy for rapidly diagnosing TB disease by identifying particular nucleotide sequences in the organism utilizing probes of labelled nucleic acid sequences.…”

Section: Molecular-based Approaches For Diagnosis and Detecting Drug ...mentioning

confidence: 99%

“…As a result, separation occurs when a link forms with the amplified target and the emitted light is detected, equivalent to the quantity of DNA in the sample. 66 This approach has the advantage of quantification of the microorganism in the clinical sample, in addition to a lower risk of contamination and a quicker turnaround time compared to Line probe assays (LPAs) due to the removal of the hybridization phase. 127 The sensitivity and specificity of RT-PCR for smear-positive TB cases are 100% and 99 %, respectively, while for smear-negative cases are 67% and 99%, respectively.…”

Section: Naat-based Tb Diagnostic Device: Genexpert Mtb/rif Assaymentioning

confidence: 99%

“…100,162 GenoType MTBDRsl VER2.0 can detect resistance to second-line anti-TB drugs such as CP, AK, and KM (rss gene), fluoroquinolones (gyrA and gyrB genes), and KAN (eis promoter gene). 66 It has variable sensitivity and specificity when screening for resistance to second-line injectable drugs. Still, it has excellent sensitivity and specificity (91% to 100%) for identifying resistance to fluoroquinolone.…”

Section: Solid-phase Hybridization or Line Probe Assaysmentioning

confidence: 99%

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Tuberculosis Diagnosis and Detection of Drug Resistance: A Comprehensive Updated Review

Tawfick,

Badawy,

Taleb

et al. 2023

J. Pure Appl. Microbiol.

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Tuberculosis (TB) is a significant public health challenge, especially in developing nations. Developing a TB eradication strategy is hampered by the global health concern of drug-resistant (DR) TB. Effective patient treatment, preventing TB transfer and avoiding the upsurge of DR strains depend primarily on the timely and accurate identification of DR TB. Due to inadequate sensitivity, the necessity of trained laboratory personnel, the sluggish growth pattern of Mycobacterium bacilli in culture, and the small number of bacilli that are usually found in extrapulmonary TB samples, TB diagnosis is still tricky in clinical practice. Although mycobacterial culture is the gold standard to identify TB and determine drug resistance, it takes 2 to 8 weeks to develop. Despite their high cost, nucleic acid amplification tests (NAATs) and whole-genome sequencing (WGS) are the commonly employed molecular-based methods for diagnosing and identifying TB. The WHO suggested the GeneXpert MTB/RIF to identify TB and detect resistance to rifampicin. In comparison, numerous molecular techniques were developed, including allele-specific PCR (MAS-PCR), solid-phase hybridization, real-time PCR (RT-PCR) and droplet digital PCR-based technique (DDPCR). This manuscript is intended to overview the current approaches for the phenotypic and genotypic diagnosis of TB disease and identifying resistance to antitubercular drugs depending on recently published articles, WHO and CDC reports, and commercially available diagnostic tools.

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“…The proportion method using Middlebrook 7H10 agar has been the "gold standard" method for several decades. 66…”

Section: Mtb Proportional Dst Methodsmentioning

confidence: 99%

Section: Molecular-based Approaches For Diagnosis and Detecting Drug ...mentioning

confidence: 99%

Section: Naat-based Tb Diagnostic Device: Genexpert Mtb/rif Assaymentioning

confidence: 99%

Section: Solid-phase Hybridization or Line Probe Assaysmentioning

confidence: 99%

See 2 more Smart Citations

Tuberculosis Diagnosis and Detection of Drug Resistance: A Comprehensive Updated Review

Tawfick,

Badawy,

Taleb

et al. 2023

J. Pure Appl. Microbiol.

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show abstract

“…The proportion method is validated for most first-and second-line TB drugs using direct (e.g., sputum) or indirect (MTB isolates from culture) specimens [21,22]. To reduce the time to DR-TB detection, Amini et al, compared the performance of this method on direct clinical specimens (e.g., smear-positive sputum samples) versus culture isolates, and found complete concordance (100% specificity and sensitivity) between the two types of specimens with a mean detection time of 20 days [23]. The WHO, however, recommends the use of culture suspension rather than a direct clinical specimen for the performance of DST.…”

Section: Phenotypic Drug Susceptibility Testing (Pdst) Assaysmentioning

confidence: 99%

Rapid Diagnosis of Drug-Resistant Tuberculosis–Opportunities and Challenges

Naidoo,

Perumal,

Ngema

et al. 2023

Pathogens

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Global tuberculosis (TB) eradication is undermined by increasing prevalence of emerging resistance to available drugs, fuelling ongoing demand for more complex diagnostic and treatment strategies. Early detection of TB drug resistance coupled with therapeutic decision making guided by rapid characterisation of pre-treatment and treatment emergent resistance remains the most effective strategy for averting Drug-Resistant TB (DR-TB) transmission, reducing DR-TB associated mortality, and improving patient outcomes. Solid- and liquid-based mycobacterial culture methods remain the gold standard for Mycobacterium tuberculosis (MTB) detection and drug susceptibility testing (DST). Unfortunately, delays to result return, and associated technical challenges from requirements for specialised resource and capacity, have limited DST use and availability in many high TB burden resource-limited countries. There is increasing availability of a variety of rapid nucleic acid-based diagnostic assays with adequate sensitivity and specificity to detect gene mutations associated with resistance to one or more drugs. While a few of these assays produce comprehensive calls for resistance to several first- and second-line drugs, there is still no endorsed genotypic drug susceptibility test assay for bedaquiline, pretomanid, and delamanid. The global implementation of regimens comprising these novel drugs in the absence of rapid phenotypic drug resistance profiling has generated a new set of diagnostic challenges and heralded a return to culture-based phenotypic DST. In this review, we describe the available tools for rapid diagnosis of drug-resistant tuberculosis and discuss the associated opportunities and challenges.

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Transcriptional regulation of T-bet, GATA3, ROR<gamma>T, HERV-K env, Syncytin-1, microRNA-9, 192 and 205 induced by nisin in colorectal cancer cell lines (SW480, HCT116) and human peripheral blood mononuclear cell

et al. 2021

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Direct drug susceptibility testing of Mycobacterium tuberculosis using the proportional method: A multicenter study

Cited by 11 publications

References 17 publications

Tuberculosis Diagnosis and Detection of Drug Resistance: A Comprehensive Updated Review

Tuberculosis Diagnosis and Detection of Drug Resistance: A Comprehensive Updated Review

Rapid Diagnosis of Drug-Resistant Tuberculosis–Opportunities and Challenges

Transcriptional regulation of T-bet, GATA3, ROR<gamma>T, HERV-K env, Syncytin-1, microRNA-9, 192 and 205 induced by nisin in colorectal cancer cell lines (SW480, HCT116) and human peripheral blood mononuclear cell

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