Direct electrochemical and AFM detection of amyloid-β peptide aggregation on basal plane HOPG

Lopes, Paula; Xu, Meng; Zhang, Min; Zhou, Ting; Yang, Yan; Wang, Chen; Ferapontova, Elena E.

doi:10.1039/c4nr02413c

Cited by 40 publications

(30 citation statements)

References 33 publications

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“…[18] Label-free electrochemical detection of Ab monomers and their aggregation is based on measuring the oxidationo ft he tyrosine (Tyr) residue at position 10 of the Ab sequence. [19][20][21] Tamiya's group firstly proposedt he electrochemical detection of Ab monomers (Ab 1-40 and Ab 1-42 ) by employing ad enselyp acked sialic acid-modified electrode which can specifically capture them. [19,22] The intrinsic oxidation signal of Ab at ap eak potential of 0.6 V( vs. Ag/AgCl) was monitored by differential pulse voltammetry.T he oxidation peak current of Ab 1-42 showed as imilar tendencya sA b 1-40 ,a nd the peak current intensities were concentration-dependent Yanli Zhou receivedh er PhD from the Te chnical Institute of Physics and Chemistry,C hinese Academy of Sciencesi n2008.…”

Section: Electrochemical Sensorsmentioning

confidence: 99%

“…Ab oligomer detection could be accomplished within one hour without any additional sample incubation. Ac onformationally sensitive bis-pyrene-labeled peptide (Ab [16][17][18][19][20][21][22][23][24][25][26][27][28][29][30][31][32][33][34][35] )w as also constructed for an oligomerspecific fluorescencea ssay. [83] Specific binding of Ab oligomers to the peptide could introduce ac hange in the fluorescence spectrumw ith ac hange of pyrene monomer fluorescence anisotropy.…”

Section: Fluorescents Ensorsmentioning

confidence: 99%

See 1 more Smart Citation

Detection of Aβ Monomers and Oligomers: Early Diagnosis of Alzheimer's Disease

Zhou

Liu

Hao

et al. 2016

Chemistry An Asian Journal

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Alzheimer's disease (AD), as the most common progressive neurodegenerative disorder, is pathologically characterized by deposition of extracellular plaque composed of amyloid-β peptide (Aβ). Different assembled states of Aβ have been considered as both important biomarkers and drug targets for the diagnosis and therapy of AD. Recent studies demonstrate that small, diffusible Aβ oligomers formed by aggregation of Aβ monomers are the major toxic agents in AD. Therefore, the development of reliable assays for Aβ (both monomers and oligomers) will be important for the early differential diagnosis of dementia, predicting the progression of AD, as well as monitoring the effectiveness of novel anti-Aβ drugs for AD. In this review, we summarize the recent progress made in the development of techniques for detection of Aβ monomers and oligomers. In particular, the principles governing the design of these sensors are classified and summarized. Moreover, the advantages and disadvantages of the assays are evaluated. This review also discusses the improvements and challenges for application of these assays in the early diagnosis of AD.

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Section: Electrochemical Sensorsmentioning

confidence: 99%

Section: Fluorescents Ensorsmentioning

confidence: 99%

Detection of Aβ Monomers and Oligomers: Early Diagnosis of Alzheimer's Disease

Zhou

Liu

Hao

et al. 2016

Chemistry An Asian Journal

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“…Correspondingly, this decrease in R ct may result from a charge injection to the HC-VGS electrode by the Aβ-Os, in particular, due to the presence of electrochemically active residues in the Aβ peptide. 50,51 Further, Figure 4(d) summarizes the changing trends in charge transfer resistance for the oligomeric and monomeric species at different concentrations. Unlike the case with Aβ-M, the presence of Aβ-O induces a significant decrease in the charge transfer resistance.…”

Section: Green Chemistrymentioning

confidence: 99%

Plasma-enabled sustainable elemental lifecycles: honeycomb-derived graphenes for next-generation biosensors and supercapacitors

et al. 2015

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ARTICLE This journal isA green and efficient conversion of redundant biomass into functional nanomaterials holds the key to sustainable future technologies. Recently, vertical graphene nanosheets (VGS) have emerged as promising nanomaterials for integration in high-performance biosensors and supercapacitors, owing to their excellent and unique structural, morphological and electrical properties. However, when considering the conventional techniques utilized in nanofabrication, such as thermal or chemical routes, these often involve complex, eco-destructive and resource-consuming processes. Here we report on a single-step, potentially scalable, environmentally-benign and plasma-enabled method to synthesize VGS from an underutilized and natural by-product precursor, honeycomb. The VGS multifunctionality is highlighted by its integration as supercapacitor electrodes for energy storage, and as an electrochemical biosensor for the detection of the neurotoxic Amyloid-beta (Aβ) biomarker of Alzheimer's disease. The VGS were employed as binder-free supercapacitor electrodes, and demonstrated high specific capacitance up to 240 F g − 1 at a scan rate of 5 mV s − 1 and 100% capacitance retention after 2,000 charge/discharge cycles. Furthermore, the VGS were functionalized with curcumin bioreceptors, and exhibited good sensitivity and selectivity towards the detection of neurotoxic Aβ species, and demonstrated a detection limit of 0.1 µ g/mL.

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“…Label-free direct electrochemical methods have been successfully applied for the study of proteins important in biomedicine including a-synuclein [11][12][13], amyloid b-peptides [14][15][16], tumor suppressor p53 [17,18], etc. Besides analysis of non-protein part in conjugated proteins [19,20], predominantly, voltammetric oxidation peaks [15,[21][22][23][24][25] and/or chronopotentiometric catalytic peak H of proteins [13,17,18,26] were used for direct electrochemistry.…”

Section: Introductionmentioning

confidence: 99%