Effect of His6-tagging of anterior gradient 2 protein on its electro-oxidation

Ostatná, Veronika; Vargová, Veronika; Hrstka, Roman; Ďurech, Michal; Vojtěšek, Bořivoj; Paleček, Emil

doi:10.1016/j.electacta.2014.10.125

Cited by 18 publications

(16 citation statements)

References 46 publications

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“…We selected for further investigation a model of the best docking solution for each of the peptides to AGR2. Such a model consisted of a representative from the top ranked cluster from the corresponding docking experiment that conformed to previous experimental observations (17,43): the N terminus of the peptide should be free, and the C terminus bound to AGR2 (supplemental Fig. S4).…”

Section: Agr2 and Pdia3/6 Interactionsmentioning

confidence: 97%

Characterization of the AGR2 Interactome Uncovers New Players of Protein Disulfide Isomerase Network in Cancer Cells

Bouchalová

Sommerová

Potěšil

et al. 2022

Molecular & Cellular Proteomics

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Section: Agr2 and Pdia3/6 Interactionsmentioning

confidence: 97%

Characterization of the AGR2 Interactome Uncovers New Players of Protein Disulfide Isomerase Network in Cancer Cells

Bouchalová

Sommerová

Potěšil

et al. 2022

Molecular & Cellular Proteomics

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“…Very recently, it has been shown that using oxidation peaks of Tyr and Trp, subpicomole amounts of a potential cancer biomarker, protein AGR2, 173 can be detected at carbon electrodes. 174 The N-terminal His-tagged and non-His-tag forms of this protein were studied, and it was found that only the His-tagged form yielded a peak of histidine. Similar results were obtained with other His-tag containing and not-containing proteins, such as α-synuclein or cytochrom b5.…”

Section: Protein Oxidation At Carbon and Other Solid Electrodesmentioning

confidence: 99%

“…Moreover, attachment of His-tagged proteins on electrodes received recently special attention in relation to forming well-organized protein layers at electrified interfaces (reviewed in ref ). Very recently, it has been shown that using oxidation peaks of Tyr and Trp, subpicomole amounts of a potential cancer biomarker, protein AGR2, can be detected at carbon electrodes . The N-terminal His-tagged and non-His-tag forms of this protein were studied, and it was found that only the His-tagged form yielded a peak of histidine.…”

Section: Protein Oxidation At Carbon and Other Solid Electrodesmentioning

confidence: 99%

Electrochemistry of Nonconjugated Proteins and Glycoproteins. Toward Sensors for Biomedicine and Glycomics

Paleček

Tkáč²,

Bartošík³

et al. 2015

Chem. Rev.

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“…At high concentrations of the proton donor, Lys and Arg strongly prevail over His contribution, while at the low proton donor concentrations His contribution can be comparable to that of Arg and Lys. By considering these dependences, contribution of His residues in proteins to CHER could be controlled making thus studies of protein His‐tagging at HMDE and SAE possible similarly as at glassy carbon electrode where oxidation of protein His‐tags was recently demonstrated 46.…”

Section: Discussionmentioning

confidence: 99%

Lysine, Arginine, and Histidine Residues in Peptide‐Catalyzed Hydrogen Evolution at Mercury Electrodes

et al. 2015

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Dedicated to Professor R. G. Compton on the Occasion of his 60 th Birthday 1IntroductionNucleic acids are electroactive species undergoing reduction and oxidation of their base residues at various types of electrodes.E lectrochemistry of nucleic acids embraces aw ide variety of natural and synthetic molecules including DNAand RNA [1][2][3].Incontrast, electrochemistry of proteins was concentrated mostly on the fast reversible electrode processes of non-protein redox centers in conjugated proteins [4][5][6][7][8].Recently,w eh ave shown that using constant-current chronopotentiometric stripping (CPS) in combination with mercury and amalgam electrodes,p ractically all proteins and many peptides produce the so-called peak H due to catalytic hydrogen evolution reaction (CHER,). Thep osition, height, shape,and area of this peak are sensitive to the analyte concentration and allow the detection of proteins in the nanomolar range. Application of CPS peak Hw as useful in the analysis of proteins such as determination of metalloproteins in tissues [12] and solubility of transmembrane proteins [13,14],m onitoring of protein aggregation [15,16] and denaturation [15,[17][18][19] [17,23].C PS peak Hi s ap owerful label-free electroanalytical signal useful in the contemporary protein research.To take full advantage of application of CPS peak Hi n the protein analysis,i ti se ssential to understand different parameters influencing the CHER. Modelp eptides [24,25] and polyamino acids [26,27] were used to explore the role of their amino acid (aa) composition [25] or interfacial organization of the adsorbed peptide layer on the CHER [28].I np eptides and proteins,o nly aa residues bearing functional groups with exchangeable protons should play the active role.W eh ave already shown that cysteine (Cys) thiol [20,24,28],l ysine (Lys) e-ammonium [27],a rginine (Arg) guanidinium or histidine (His) imidazolium groups [25] transfer the protons from the acid constituent of the buffer (proton donor) onto highly negatively charged surface of the electrode where they subsequently combine in more stable molecules of the gaseous hydrogen (H 2 ); for more details about the mechanism of the catalytic hydrogen evolution see e.g. [9].Thea bility of proteins to catalyze hydrogen evolution from aqueous solutions was discovered [29] soon after invention of polarography.T he so-called presodium wave on DC polarograms was too close to the background discharge and was considered of little analytical use.I tw as shown that the catalytic process is better developed in buffered media and application of polarography in studying the catalytic process was restricted to neutral or weakly alkaline media;a tp H < 7t his wave was masked by hydrogen evolution from the buffer [30].C ompared to DC polarography,c hronopotentiometry is highly sensitive to the catalysis of hydrogen evolution and CPS peak Hi s well separated from the background discharge even below pH 7[ 31].T his peak can be considered as as econd generation of the presodium wave [32,33].Recently,w e...

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Effect of His6-tagging of anterior gradient 2 protein on its electro-oxidation

Cited by 18 publications

References 46 publications

Characterization of the AGR2 Interactome Uncovers New Players of Protein Disulfide Isomerase Network in Cancer Cells

Characterization of the AGR2 Interactome Uncovers New Players of Protein Disulfide Isomerase Network in Cancer Cells

Electrochemistry of Nonconjugated Proteins and Glycoproteins. Toward Sensors for Biomedicine and Glycomics

Lysine, Arginine, and Histidine Residues in Peptide‐Catalyzed Hydrogen Evolution at Mercury Electrodes

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