Direct electrochemical oxidation of proteins at conductive diamond electrodes

Chiku, Masanobu; Ivandini, Tribidasari A.; Kamiya, Akihide; Fujishima, Akira; Einaga, Yasuaki

doi:10.1016/j.jelechem.2007.09.027

Cited by 75 publications

(37 citation statements)

References 21 publications

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“…Similarly, using boron doped diamond electrode, native and denatured BSA could be distinguished. 219 , 220 Topal et al 220 showed oxidation signals of native and denatured macroglobulin at a gold electrode close to 0.7 V. However, a basal plane carbon electrode displayed poor ability to distinguish between these forms of HSA. 221 Using EPGE, a number of proteins were tested, showing much higher oxidation peaks in denatured than in their native forms.…”

Section: Protein Oxidation At Carbon and Other Solid Electrodesmentioning

confidence: 99%

Electrochemistry of Nonconjugated Proteins and Glycoproteins. Toward Sensors for Biomedicine and Glycomics

Paleček

Tkáč²,

Bartošík³

et al. 2015

Chem. Rev.

289

276

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Section: Protein Oxidation At Carbon and Other Solid Electrodesmentioning

confidence: 99%

Electrochemistry of Nonconjugated Proteins and Glycoproteins. Toward Sensors for Biomedicine and Glycomics

Paleček

Tkáč²,

Bartošík³

et al. 2015

Chem. Rev.

289

276

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“…It was previously reported that Bovine Serum Albumin (BSA) are electroactive at hydrogen terminated BDD but not at oxidized terminated BDD electrodes. 38 Since the oxidized terminated one was employed in this work, it can be presumed that the oxidation peaks that appeared at the CVs were related to the oxidation of biotin-streptavidin systems. Furthermore, these peaks appeared at different potentials with very small currents in comparison with the thiocholine peak.…”

Section: Modification Of Magnetic Beads With Achementioning

confidence: 99%

Magnetic Enzymatic Platform for Organophosphate Pesticide Detection Using Boron-doped Diamond Electrodes

et al. 2015

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A simple and reliable enzymatic system for organophosporus pesticide detection was successfully developed, by exploiting the synergy between the magnetic beads collection capacity and the outstanding electrochemistry property of boron-doped diamond electrodes. The determination of an organophosphate pesticide, chlorpyrifos (CPF), was performed based on the inhibition system of the enzyme acetylcholinesterase bonded to magnetic beads through a biotin-streptavidin complex system. A better sensitivity was found for a system with magnetic beads in the concentration range of 10(-9) to 10(-5) M. The estimated limits of detection based on IC10 (10% acetylcholinesterase (AChE) inhibition) have been detected and optimized to be 5.7 × 10(-10) M CPF. Spiked samples of water of Yokohama (Japan) have been measured to validate the efficiency of the enzymatic system. The results suggested that the use of magnetic beads to immobilize biomolecules or biosensing agents is suitable to maintain the superiority of BDD electrodes.

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“…This result is reasonable since mucin contains proteins, which can provide some electroactive functional groups. 34,35 The presence of mucin also seems to give current contributions as showed by higher slopes for both oxidation and reduction peak currents. However, a comparable LODs was achieved, i.e.…”

Section: Electrochemical Detection Of Neuraminidase (Na)mentioning

confidence: 99%

Electrochemical Behavior of Zanamivir at Gold-Modified Boron-Doped Diamond Electrodes for an Application in Neuraminidase Sensing

et al. 2015

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This study emphasizes the electrochemical detection of Neuraminidase (NA) based on NA inhibition by Zanamivir. The detection method was developed based on the difference of electrochemical responses of Zanamivir in the presence and the absence of NA in phosphate buffer solution (pH 5.5). Gold and gold-modified boron-doped diamond was used as the working electrode and the measurement was conducted using cyclic voltammetry method. A linear calibration curve of Zanamivir was observed in the concentration range of 5 × 10−4 mol/L (R 2 = 0.991), with the estimated limit of detection (LOD) of 1.49 × 10 −6 mol/L. The presence of NA increases the peak current of gold oxidation and reduction with linear calibration curves were monitored in the concentration range of 0-15 mU (R 2 = 0.996). An estimated LOD of 0.25 mU NA and an excellent reproducibility of the detection with an RSD of 1.18% can be achieved. Application of a real sample was successfully demonstrated for NA detection in the presence of mucin, suggested that the method is promising for pharmaceutical or medical application.

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Direct electrochemical oxidation of proteins at conductive diamond electrodes

Cited by 75 publications

References 21 publications

Electrochemistry of Nonconjugated Proteins and Glycoproteins. Toward Sensors for Biomedicine and Glycomics

Electrochemistry of Nonconjugated Proteins and Glycoproteins. Toward Sensors for Biomedicine and Glycomics

Magnetic Enzymatic Platform for Organophosphate Pesticide Detection Using Boron-doped Diamond Electrodes

Electrochemical Behavior of Zanamivir at Gold-Modified Boron-Doped Diamond Electrodes for an Application in Neuraminidase Sensing

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