Direct electrochemical sensor for fast reagent-free DNA detection

Комарова, Е. В.; Aldissi, Matt; Bogomolova, Anastasia

doi:10.1016/j.bios.2004.07.025

Cited by 75 publications

(35 citation statements)

References 20 publications

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“…Langmuir-Blodgett (LB) method is a convenient tool for designing artificial films with biological roles and has been applied to the fabrication of enzyme sensors [15], biomolecular microphotodiode [16], and biocatalysis membrane [17]. As far as we know, the report regarding immobilizing DNA by LB technique to design a voltammetric sensor is raised [18,19] but very limited.…”

Section: Introductionmentioning

confidence: 98%

Immobilization of DNA on a glassy carbon electrode based on Langmuir–Blodgett technique: application to the detection of epinephrine

Wang

et al. 2012

J Solid State Electrochem

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A double-stranded (ds) DNA-octadecylamine Langmuir-Blodgett film was attached to the surface of glassy carbon electrode (GCE) to create a novel voltammetric sensor (DNA-LB/GCE) for epinephrine (EP). Atomic force microscopy and electrochemical impedance spectroscopy were employed to study the characteristic of the DNA-LB film. The electrochemical behavior of EP at the modified electrode was investigated in pH 6.0 phosphate buffer solutions by cyclic voltammetry and amperometric methods. Compared with bare GCE, the DNA-LB/GCE sensor demonstrated an electrocatalytic effect on the oxidation of EP. In addition, the sensor shows excellent selectivity for EP detection, being free of interference from excess ascorbic acid and uric acid, and the method was also applied successfully to detect EP in the human urine samples.

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Section: Introductionmentioning

confidence: 98%

Immobilization of DNA on a glassy carbon electrode based on Langmuir–Blodgett technique: application to the detection of epinephrine

Wang

et al. 2012

J Solid State Electrochem

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“…Thus the suggested nanoparticle signal amplification was very efficient for ultrasensitive electrochemical detection of DNA hybridization. Compared to other electrochemical methods [42][43][44][45] without the signal amplification, this proposed nanoparticle-based method showed a much-lower limit of detection, and was competitive with the highly sensitive detection of DNA by amplifying the sample with PCR technique. [46] In addition, this method was superior to some amplification techniques, such as liposome (0.75 amol), [28] magnetic particles (5.7 fmol), [47] bio-barcodes (2.5 fmol L À1 ) [48] and Au-NP-based amplification (10 zmol).…”

Section: Stripping Voltammetric Analysis Of Target Dnamentioning

confidence: 99%

Quantum‐Dot‐Functionalized Poly(styrene‐co‐acrylic acid) Microbeads: Step‐Wise Self‐Assembly, Characterization, and Applications for Sub‐femtomolar Electrochemical Detection of DNA Hybridization

Dong

Yan

et al. 2010

Adv Funct Materials

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A novel nanoparticle label capable of amplifying the electrochemical signal of DNA hybridization is fabricated by functionalizing poly(styrene‐co‐acrylic acid) microbeads with CdTe quantum dots. CdTe‐tagged polybeads are prepared by a layer‐by‐layer self‐assembly of the CdTe quantum dots (diameter = 3.07 nm) and polyelectrolyte on the polybeads (diameter = 323 nm). The self‐assembly procedure is characterized using scanning and transmission electron microscopy, and X‐ray photoelectron, infrared and photoluminescence spectroscopy. The mean quantum‐dot coverage is (9.54 ± 1.2) × 103 per polybead. The enormous coverage and the unique properties of the quantum dots make the polybeads an effective candidate as a functionalized amplification platform for labelling of DNA or protein. Herein, as an example, the CdTe‐tagged polybeads are attached to DNA probes specific to breast cancer by streptavidin–biotin binding to construct a DNA biosensor. The detection of the DNA hybridization process is achieved by the square‐wave voltammetry of Cd2+ after the dissolution of the CdTe tags with HNO3. The efficient carrier‐bead amplification platform, coupled with the highly sensitive stripping voltammetric measurement, gives rise to a detection limit of 0.52 fmol L−1 and a dynamic range spanning 5 orders of magnitude. This proposed nanoparticle label is promising, exhibits an efficient amplification performance, and opens new opportunities for ultrasensitive detection of other biorecognition events.

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“…In the immobilization technique, it is necessary that the binding chemistry is stable during subsequent assay steps; the sequence of the DNA probe should not change the chemical structure, and the bio-recognition molecules have to be attached with an appropriate orientation. Nowadays, various methods are used to immobilize the DNA strands on the sensor surface, such as the covalent bonds to the functionalized support [73], electrochemical [74], physical absorption [75] and monolayer self-assembling [76]. Among these methods, the covalent bond induced immobilization provides advantages over other methods in terms of simplicity, efficiency, ordered binding, and low cost.…”

Section: Cnt/dna-based Nanosensorsmentioning

confidence: 99%

Dispersions Based on Carbon Nanotubes – Biomolecules Conjugates

Capek¹

2011

Carbon Nanotubes - Growth and Applications

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Direct electrochemical sensor for fast reagent-free DNA detection

Cited by 75 publications

References 20 publications

Immobilization of DNA on a glassy carbon electrode based on Langmuir–Blodgett technique: application to the detection of epinephrine

Immobilization of DNA on a glassy carbon electrode based on Langmuir–Blodgett technique: application to the detection of epinephrine

Quantum‐Dot‐Functionalized Poly(styrene‐co‐acrylic acid) Microbeads: Step‐Wise Self‐Assembly, Characterization, and Applications for Sub‐femtomolar Electrochemical Detection of DNA Hybridization

Dispersions Based on Carbon Nanotubes – Biomolecules Conjugates

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