Direct enzyme-linked aptamer assay (DELAA) for diagnosis of toxoplasmosis by detection of SAG1 protein in mice and humans

Cui, Wen; Shen, Xuhang; Bibi, Asma; Cudjoe, Obed; Zhao, Liang; Yu, Li; Du, Jian; Xu, Yuanhong; Chen, Xi; Shen, Jilong; Wang, Wei

doi:10.1016/j.actatropica.2021.106255

Cited by 5 publications

(7 citation statements)

References 31 publications

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“…An alternative method for the diagnosis of toxoplasma infection is to examine circulating antigens. Surface antigen 1 (SAG1) is the most explored and used tachyzoite stage-specific antigen of T. gondii for serological test development (see Figure 1 ) [ 43 ]. SAG1 is a significant parasite surface protein which plays a role as a critical virulence factor.…”

Section: Diagnostics Of Toxoplasmosismentioning

confidence: 99%

“…This protein induces both humoral and cellular host immune responses [ 14 ]. DELAA is one of the novel methods developed to detect SAG1 [ 43 ]. This method uses aptamers, termed “chemical antibodies”, which characterize high binding affinity and specificity [ 45 ].…”

Section: Diagnostics Of Toxoplasmosismentioning

confidence: 99%

“…This method uses aptamers, termed “chemical antibodies”, which characterize high binding affinity and specificity [ 45 ]. Additionally, a real-time polymerase chain reaction (real-time PCR) assay using a set of primers targeting the SAG1 gene showed a high sensitivity for the fast and specific detection of parasites [ 43 ].…”

Section: Diagnostics Of Toxoplasmosismentioning

confidence: 99%

“…Two INAAT methods, namely Loop-Mediated Isothermal Amplification (LAMP) and Nucleic Acid Sequence-Based Amplification (NASBA), have been used for the rapid detection of Toxoplasma gondii [ 33 , 59 ]. LAMP is based upon the amplification of DNA (or RNA if combined with a retro-transcription step) under isothermal conditions (63–65 °C) [ 43 ]. This method, initially developed by Notomi et al in 2000 [ 60 ], is based on the reaction of DNA polymerase with strand displacement activity and a set of two specially designed inner primers (forward inner primer, FIP, and backward inner primer, BIP) and two outer primers (forward primer F3 and backward primer B3) that amplifies DNA rapidly with high efficiency and specificity.…”

Section: Diagnostics Of Toxoplasmosismentioning

confidence: 99%

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Advances and Challenges in Diagnostics of Toxoplasmosis in HIV-Infected Patients

et al. 2023

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Toxoplasma gondii is a worldwide distributed protozoan parasite. This apicomplexan parasite infects one-third of the population worldwide, causing toxoplasmosis, considered one of the neglected parasitic infections. In healthy humans, most infections are asymptomatic. However, in immunocompromised patients, the course of the disease can be life-threatening. Human immunodeficiency virus (HIV)-infected patients have a very high burden of Toxoplasma gondii co-infection. Thus, it is essential to use modern, sensitive, and specific methods to properly monitor the course of toxoplasmosis in immunodeficient patients.

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Section: Diagnostics Of Toxoplasmosismentioning

confidence: 99%

Section: Diagnostics Of Toxoplasmosismentioning

confidence: 99%

Section: Diagnostics Of Toxoplasmosismentioning

confidence: 99%

Section: Diagnostics Of Toxoplasmosismentioning

confidence: 99%

See 2 more Smart Citations

Advances and Challenges in Diagnostics of Toxoplasmosis in HIV-Infected Patients

et al. 2023

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“…Finally, the DELAA was further evaluated with 15 positive and 35 negative human sera samples. In this test, the DELAA showcased a high sensitivity of 93.33% and specificity of 94.29% [ 70 ].…”

Section: Aptasensors For Parasite Detectionmentioning

confidence: 99%

Aptamer-Based Technologies for Parasite Detection

Brosseau

Vallée

Mayer‐Scholl

et al. 2023

Sensors

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Centuries of scientific breakthroughs have brought us closer to understanding and managing the spread of parasitic diseases. Despite ongoing technological advancements in the detection, treatment, and control of parasitic illnesses, their effects on animal and human health remain a major concern worldwide. Aptamers are single-stranded oligonucleotides whose unique three-dimensional structures enable them to interact with high specificity and affinity to a wide range of targets. In recent decades, aptamers have emerged as attractive alternatives to antibodies as therapeutic and diagnostic agents. Due to their superior stability, reusability, and modifiability, aptamers have proven to be effective bioreceptors for the detection of toxins, contaminants, biomarkers, whole cells, pathogens, and others. As such, they have been integrated into a variety of electrochemical, fluorescence, and optical biosensors to effectively detect whole parasites and their proteins. This review offers a summary of the various types of parasite-specific aptamer-based biosensors, their general mechanisms and their performance.

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Current developments of SELEX technologies and prospects in the aptamer selection with clinical applications

Chinchilla-Cárdenas,

Cruz-Méndez,

Petano-Duque

et al. 2024

Journal of Genetic Engineering and Biotechnology

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Direct enzyme-linked aptamer assay (DELAA) for diagnosis of toxoplasmosis by detection of SAG1 protein in mice and humans

Cited by 5 publications

References 31 publications

Advances and Challenges in Diagnostics of Toxoplasmosis in HIV-Infected Patients

Advances and Challenges in Diagnostics of Toxoplasmosis in HIV-Infected Patients

Aptamer-Based Technologies for Parasite Detection

Current developments of SELEX technologies and prospects in the aptamer selection with clinical applications

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