Direct enzyme-linked immunosorbent assay and a radioimmunoassay for melatonin compared

Chegini, Soheil; Ehrhart-Hofmann, B.; Kaider, Alexandra; Waldhauser, Franz

doi:10.1093/clinchem/41.3.381

Cited by 35 publications

(6 citation statements)

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“…There are also numerous enzyme-linked immunoassay (ELISA) commercial kits used for the analysis of melatonin in urine, blood, and saliva. A commercial ELISA kit was compared to the RIA method: a purification step (not specified by the manufacturer) was necessary to obtain good correlations with RIA for monitoring human blood melatonin [ 125 ]. In this study, the ELISA method presented a mean inter-assay CV of 15.4%, but it is more convenient than RIA (no radioactive wastes).…”

Section: Measure Of Melatonin Concentrations Depending On the Typementioning

confidence: 99%

Melatonin: From Pharmacokinetics to Clinical Use in Autism Spectrum Disorder

Lalanne

Fougerou‐Leurent

Anderson

et al. 2021

IJMS

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The role of melatonin has been extensively investigated in pathophysiological conditions, including autism spectrum disorder (ASD). Reduced melatonin secretion has been reported in ASD and led to many clinical trials using immediate-release and prolonged-release oral formulations of melatonin. However, melatonin’s effects in ASD and the choice of formulation type require further study. Therapeutic benefits of melatonin on sleep disorders in ASD were observed, notably on sleep latency and sleep quality. Importantly, melatonin may also have a role in improving autistic behavioral impairments. The objective of this article is to review factors influencing treatment response and possible side effects following melatonin administration. It appears that the effects of exposure to exogenous melatonin are dependent on age, sex, route and time of administration, formulation type, dose, and association with several substances (such as tobacco or contraceptive pills). In addition, no major melatonin-related adverse effect was described in typical development and ASD. In conclusion, melatonin represents currently a well-validated and tolerated treatment for sleep disorders in children and adolescents with ASD. A more thorough consideration of factors influencing melatonin pharmacokinetics could illuminate the best use of melatonin in this population. Future studies are required in ASD to explore further dose-effect relationships of melatonin on sleep problems and autistic behavioral impairments.

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Section: Measure Of Melatonin Concentrations Depending On the Typementioning

confidence: 99%

Melatonin: From Pharmacokinetics to Clinical Use in Autism Spectrum Disorder

Lalanne

Fougerou‐Leurent

Anderson

et al. 2021

IJMS

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“…Direct melatonin immunoassays rely very heavily on the specificity of the antibody and the absence of any nonspecific matrix effects. In 1995, Chegini et al compared the performance of a direct ELISA and an extraction RIA using human plasma samples. The ELISA used a rabbit anti‐melatonin antibody purchased from Buhlmann Laboratories and peroxidase‐conjugated melatonin, while the RIA used antiserum purchased from CID‐Tech and chloroform extraction and a petroleum ether wash.…”

Section: Issues To Consider When Conducting Melatonin Studiesmentioning

confidence: 99%

A critical review of melatonin assays: Past and present

Kennaway

2019

Journal of Pineal Research

110

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There has been increased interest in the measurement of melatonin in plasma and saliva recently either as a marker of circadian phase or to understand the physiological role of melatonin. For both situations, there is a need for a specific assay for melatonin that is sensitive enough to detect low concentrations (<2 pg/mL). Since the mid‐1970s, there have been many assays developed to measure melatonin in blood and saliva. Radioimmunoassays and ELISA have predominated because of their relative simplicity and high throughput. In this review, I show that the early radioimmunoassays while providing valuable information about nocturnal melatonin levels in humans, generally produced inaccurate basal (daytime) levels. Mass spectrometry assays, however, have provided us with the target values that immunoassays need to achieve, that is, daytime plasma melatonin levels <1 pg/mL. There are now many contemporary commercial assays available utilising both RIA and ELISA technologies, but not all achieve the standards set by the mass spectrometry assays. The performance of these assays is reviewed. I conclude with recommendations on issues researchers need to consider when conducting melatonin studies, including the importance of time of day of collection, validation of assays, the potential causes of poor assay specificity at low levels, the advantages/disadvantages of using saliva vs plasma and extraction assays vs direct assays, kit manufacturers responsibilities and the reporting requirements when publishing melatonin studies.

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“…Melatonin can be detected through several methods, such as radioimmunoassay (RIA) [13,[27][28][29], enzymelinked immunosorbent assay (ELISA) [28,[30][31][32] and highperformance liquid chromatography (HPLC) [19,33]. In order to increase the validity of results, determination of melatonin was performed successfully using mass spectrometry [34].…”

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confidence: 99%

Sensitive Electrochemical Detection Method of Melatonin in Food Supplements

Miccoli¹,

Restani²,

Floroian³

et al. 2018

Rev. Chim.

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Melatonin (N-acetyl-5-methoxytryptamin) is a ubiquitous molecule widely distributed in nature. It is a hormone produced by the pineal gland, which plays a role in the body�s sleep cycles and it is a powerful antioxidant. It is possible to find it in several foods (fruit, rice, corn) and as food supplement. Screen-printed carbon electrode (DRP-150) and graphene modified screen-printed electrode (DRP-110GPH) were tested as sensors for the detection of melatonin using electrochemical detection (differential pulse voltammetry), at different pH values (7.4, 7.0, and 6.4). Quantitative detection of melatonin was possible, with better results when graphene modified screen-printed electrodes were used. The pH influenced the position of the peak as well, with lowering the pH moves more in the right. Both electrodes have been tested on samples of food supplements containing melatonin, with good recovery degrees.

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Direct enzyme-linked immunosorbent assay and a radioimmunoassay for melatonin compared

Cited by 35 publications

References 0 publications

Melatonin: From Pharmacokinetics to Clinical Use in Autism Spectrum Disorder

Melatonin: From Pharmacokinetics to Clinical Use in Autism Spectrum Disorder

A critical review of melatonin assays: Past and present

Sensitive Electrochemical Detection Method of Melatonin in Food Supplements

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