Direct evidence for the non-random localization of mammalian chromosomes in the interphase nucleus

Hadlaczky, Gyula; Went, M; Ringertz, Nils R.

doi:10.1016/0014-4827(86)90199-0

Cited by 93 publications

(27 citation statements)

References 24 publications

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“…In agreement with this investigation laser uv-microirradiation experiments also argue against the close and permanent association of homologous chromosomes in fibroblastoid Chinese hamster cells [42,431. In contrast Hadlaczky et al [44] reported that homologous chromosomes recognized by immunostained centromeres occupy adjacent territories in 30 % of Indian muntjac cells in which centromeres of individual chromosomes could be identified. Differences in the methods applied in these investigations do not suffice in our opinion to explain these and other findings (see also below).…”

Section: Discussionmentioning

confidence: 84%

Double in situ hybridization in combination with digital image analysis: A new approach to study interphase chromosome topography

Emmerich

Loos

Jauch

et al. 1989

Experimental Cell Research

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Double in situ hybridization with mercurated and biotinylated chromosome specific DNA probes in combination with digital image analysis provides a new approach to compare the distribution of homologous and nonhomologous chromosome targets within individual interphase nuclei. Here we have used two DNA probes representing tandemly repeated sequences specific for the constitutive heterochromatin of the human chromosomes 1 and 15, respectively, and studied the relative arrangements of these chromosome targets in interphase nuclei of human lymphocytes, amniotic fluid cells, and fibroblasts, cultivated in vitro. We have developed a ZD-image analysis approach which allows the rapid evaluation of large numbers of interphase nuclei. Models to test for a random versus nonrandom distribution of chromosome segments are discussed taking into account the three-dimensional origin of the evaluated 2D-distribution. In all three human diploid cell types the measurements of target-target and target-center distances in the ZD-nuclear image revealed that the labeled segments of the two chromosomes 15 were distributed both significantly closer to each other and closer to the center of the nuclear image than the labeled chromosome 1 segments. This result can be explained by the association of nucleolus organizer regions on the short arm of chromosome 15 with nucleoli located more centrally in these nuclei and does not provide evidence for a homologous association per se. In contrast, evaluation of the interphase positioning of the two chromosome 1 segments fits the random expectation in amniotic fluid and fibroblast cells, while in experiments using lymphocytes a slight excess of larger distances between these homologous targets was occasionally observed. 2D-distances between the labeled chromosome 1 and 15 segments showed a large variability in their relative positioning. In conclusion our data do not support the idea of a strict and permanent association of these homologous and nonhomologous targets in the cell types studied so far.

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Section: Discussionmentioning

confidence: 84%

Double in situ hybridization in combination with digital image analysis: A new approach to study interphase chromosome topography

Emmerich

Loos

Jauch

et al. 1989

Experimental Cell Research

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“…Immunocytochemistry was performed as described (Kouznetsova et al, 2005) using a 'drying-down' technique (Peters et al, 1997) and the following antibodies: rabbit anti-SYCP1 (1:50) (Liu et al, 1996), mouse anti-SYCP1 (1:200) (gift from C. Heyting, Wageningen, The Netherlands,), rabbit anti-SYCP3 (1:200) (Liu et al, 1996), human anti-CREST (1:1500) (Hadlaczky et al, 1986), rabbit anti-STAG3 (1:400) (Pezzi et al, 2000), guinea pig anti-STAG3 (1:200) (Kouznetsova et al, 2005), guinea pig anti-SYCE1 (1:1500) (Hamer et al, 2006), guinea pig anti-SYCE2 (1:400) (Hamer et al, 2006), rabbit anti-γ-H2AX (Upstate Biotechnology) (1:100), rabbit anti-BRCA1 (gift from J. M. A. Turner, National Institute for Medical Research, London, UK) (1:1000), rabbit anti-DMC1 (1:100) and rabbit anti-RPA (1:500) (gifts from P. Moens, York University, Toronto, Canada) and mouse anti-MLH1 (1:50) (BD Biosciences). Secondary antibodies were applied as described (Kouznetsova et al, 2005;Wang and Hoog, 2006).…”

Section: Immunohistochemistry Immunocytochemistry and Electron Micromentioning

confidence: 99%

Progression of meiotic recombination requires structural maturation of the central element of the synaptonemal complex

Hamer

Wang

Bolcun-Filas

et al. 2008

Journal of Cell Science

120

160

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The synaptonemal complex is an elaborate meiosis-specific supramolecular protein assembly that promotes chromosome synapsis and meiotic recombination. We inactivated the meiosis-specific gene Tex12 and found that TEX12 is essential for progression of meiosis in both male and female germ cells. Structural analysis of the synaptonemal complex in Tex12–/– meiocytes revealed a disrupted central element structure, a dense structure residing between the synapsed homologous chromosomes. Chromosome synapsis is initiated at multiple positions along the paired homologous chromosomes in Tex12–/– meiotic cells, but fails to propagate along the chromosomes. Furthermore, although meiotic recombination is initiated in Tex12–/– meiotic cells, these early recombination events do not develop into meiotic crossovers. Hence, the mere initiation of synapsis is not sufficient to support meiotic crossing-over. Our results show that TEX12 is a component of the central element structure of the synaptonemal complex required for propagation of synapsis along the paired homologous chromosomes and maturation of early recombination events into crossovers.

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“…Immunocytochemistry was performed as described (Kouznetsova et al, 2005) using a 'drying-down' technique (Peters et al, 1997) and the following antibodies: rabbit anti-SYCP1 (1:50) (Liu et al, 1996), rabbit anti-SYCP3 (1:200) (Liu et al, 1996), human anti-CREST (1:1500) (Hadlaczky et al, 1986), rabbit anti-STAG3 (Pezzi et al, 2000), guinea pig anti-SYCE1 (1:1500) and -SYCE2 (1:400), rabbit anti-SYCE1 (1:500) and anti-SYCE2 (1:100) (Costa et al, 2005) and guinea pig anti-TEX12 (1:200). Secondary antibodies were applied as described (Kouznetsova et al, 2005).…”

Section: Immunohistochemistry Immunocytochemistry and Electron Micromentioning

confidence: 99%

Characterization of a novel meiosis-specific protein within the central element of the synaptonemal complex

Hamer

Gell

Kouznetsova

et al. 2006

Journal of Cell Science

146

157

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During the first meiotic prophase, alignment and synapsis of the homologous chromosomes are mediated by the synaptonemal complex. Incorrect assembly of this complex results in cell death, impaired meiotic recombination and formation of aneuploid germ cells. We have identified a novel mouse meiosis-specific protein, TEX12, and shown it to be a component of the central element structure of the synaptonemal complex at synapsed homologous chromosomes. Only two other central element proteins, SYCE1 and SYCE2, have been identified to date and, using several mouse knockout models, we show that these proteins and TEX12 specifically depend on the synaptonemal transverse filament protein SYCP1 for localization to the meiotic chromosomes. Additionally, we show that TEX12 exactly co-localized with SYCE2, having the same, often punctate, localization pattern. SYCE1, on the other hand, co-localized with SYCP1 and these proteins displayed the same more continuous expression pattern. These co-localization studies were confirmed by co-immunoprecipitation experiments that showed that TEX12 specifically co-precipitated with SYCE2. Our results suggest a molecular network within the central elements, in which TEX12 and SYCE2 form a complex that interacts with SYCE1. SYCE1 interacts more directly with SYCP1 and could thus anchor the central element proteins to the transverse filaments.

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Direct evidence for the non-random localization of mammalian chromosomes in the interphase nucleus

Cited by 93 publications

References 24 publications

Double in situ hybridization in combination with digital image analysis: A new approach to study interphase chromosome topography

Double in situ hybridization in combination with digital image analysis: A new approach to study interphase chromosome topography

Progression of meiotic recombination requires structural maturation of the central element of the synaptonemal complex

Characterization of a novel meiosis-specific protein within the central element of the synaptonemal complex

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