Direct exposure to mild heat stress stimulates cell viability and heat shock protein expression in primary cultured broiler fibroblasts

Siddiqui, Sharif Hasan; Subramaniyan, Sivakumar Allur; Kang, Darae; Park, Jinryong; Khan, Mousumee; Choi, Hyun Woo; Shim, Kwan Seob

doi:10.1007/s12192-020-01140-x

Cited by 18 publications

(11 citation statements)

References 44 publications

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“…This temperature-time combination resulted in the highest PBMC viability, close to that of the control samples (the ones which were not exposed to heat shock). This scenario further suggests that a moderate in vitro thermal condition (37°C for 3 h) mimicked the mammalian BT or provided the BT required by mammals for proper cellular functioning and survival [6]. The present study shows that cells performed better under a mild in vitro thermal condition of 37°C and further confirms that 37°C is the normal BT required for optimal biological functions of the body.…”

Section: Numbers Ofsupporting

confidence: 75%

“…Senescence-related thermal assaults have been reported to induce a loss of the cell's power to divide and impair cellular functions, including the immunomodulatory activity of immune response cells [16]. Siddiqui et al [6] reported that under extreme TACs, the cellular system suffers a heat shock and as such defense mechanisms become too weak to respond to TS and opportunistic infections. In the present study, we evidenced a direct relationship between harsher in vitro TACs and the lower number of PBMCs, whereas no such relationship was found in control samples exposed to no TAC and DTE.…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies [6][7][8][9] reported the effects of variations in thermal assault conditions (TACs) and heat shock on cellular integrity, proliferation, and viability as well as on RNA concentration, thus predisposing animals to vulnerable opportunistic infections resulting from immune response breakdown and decline in production performance and reproduction. RNA as a nucleic acid is heat labile and becomes very unstable and very unstable under harsh environmental conditions, particularly thermal conditions.…”

Section: Introductionmentioning

confidence: 99%

“…Indeed, there is a need to mitigate the consequences of harsh environmental circumstances to enable livestock to perform better in terms of production and reproduction capabilities [3]. A first focus might be on improving traits such as fertility, conception rates, feed intake, growth, milk production, meat production, and animal health [6]. To achieve this, further biological information including various genomic regions and cellular systems enabling a wide range of farm animals to withstand TS must be obtained [2].…”

Section: Introductionmentioning

confidence: 99%

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Exposure to high thermal conditions for a long time induces apoptosis and decreases total RNA concentration in peripheral blood mononuclear cells among Indian Zebu–Jersey crossbreds

et al. 2022

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Background and Aim: Global warming has grave consequences on livestock production systems and profound negative effects on animal production. This study aimed to carry out an in vitro thermal stress stimulation (TSS) of bovine peripheral blood mononuclear cells (PBMCs) using different thermal assault conditions (TACs), including normal to extreme temperatures and varying durations of thermal exposure (DTE) to understand how PBMCs of Indian Zebu–Jersey crossbreds respond to various levels and durations of heat shock. Materials and Methods: Ten milliliters of blood were collected from 70 Indian Zebu–Jersey crossbreds under aseptic conditions and were sampled for isolating PBMCs. Peripheral blood mononuclear cells were divided into seven groups, each comprising 10 PBMC samples isolated from 10 different animals. Aliquots of 500 μL of PBMCs were stressed by exposure to different TACs (37, 40, and 45°C) for DTEs of 3 or 6 h. Subsequently, the cells were harvested. The control unstressed samples (500 μL aliquots of PBMCs) were exposed to no TAC (0°C) and zero DTE (0 h). Total RNA from all the treatment groups of PBMCs were isolated and quantitated. Results: We found a very strong association between TACs and RNA levels. In addition, PBMCs viability was negatively affected by heat shock. This led to an exponential reduction in PBMC count as TACs toughened. Only 3.59 × 105 ± 0.34 cells/mL were viable after exposure to 45°C for a 6 h DTE. This cell viability was lower than that measured in controls subjected to no stress and zero time DTE (2.56 × 107 ± 0.22 cells/mL). We also observed a reduction in the concentration of RNA isolated from thermally stressed PBMCs. Conclusion: In vitro TSS of PBMCs provided biological information on the response of cellular systems to heat shock after exposure to TACs. This will help to mitigate and manage the effects of thermal stress in bovine species. The association between the reduction in PBMC count after in vitro TSS and the expression of heat shock protein 70 gene will be investigated in the future to further understand how Indian Zebu–Jersey crossbreds respond to in vitro thermal conditions. This will be used to determine the in vivo response of Indian Jersey crossbreds to different environmental thermal conditions and will further enable the in vivo understanding of thermotolerance potentials of bovine species for better adaptation, survival, and production performance.

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Section: Numbers Ofsupporting

confidence: 75%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Exposure to high thermal conditions for a long time induces apoptosis and decreases total RNA concentration in peripheral blood mononuclear cells among Indian Zebu–Jersey crossbreds

et al. 2022

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“…As a consequence, this increases the disease susceptibility of broilers (Monson et al 2018), which leads to a high mortality rate (Brossi et al 2018). Moreover, the high temperature reduces the broiler muscle cell proliferation (Siddiqui et al 2020b, Siddiqui et al 2021b) by altering the molecular structure and function of the intestine. Our result is consistent with the results of a previous study, indicating that environmental factors reduce the growth performance of broilers (Zaghari et al 2011).…”

Section: Discussionmentioning

confidence: 99%

Growth performance of broilers and prevalence of different diseases vary with environmental variation

Ahmed¹,

Siddiqui

Das

et al. 2022

Preprint

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Broiler meat is known for its nutritional value, availability, and low price. Although, broilers are more sensitive to environmental conditions and need a specific environment for optimum production. However, global warming and natural disasters are important obstacles to broiler production. Therefore, we conducted this study to reveal the broiler production status throughout the year in a subtropical region. In our study, we selected the years 2019, 2020, and 2022. The study area included Noakhali and Lakshimpur, located in the southern part of Bangladesh. We selected 10 smallholder broiler farms following specific conditions and collected information at the end of each month for analysis. The body weight of broilers was significantly lower in July than in other months. However, the mortality was significantly lower in July than in other months of the year. Nonetheless, the prevalence of viral disease was higher in the winter season (January, February, and December). Although, the metabolic disease prevalence was higher in October, a high prevalence of bacterial disease was found in April. These results suggest that high temperatures decrease broiler production while broilers are more susceptible to viral disease in the winter season.

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Closed‐Loop Control of Macrophage Engineering Enabled by Focused‐Ultrasound Responsive Mechanoluminescence Nanoplatform for Precise Cancer Immunotherapy

Xu,

Meng,

et al. 2024

Small

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Macrophage engineering has emerged as a promising approach for modulating the anti‐tumor immune response in cancer therapy. However, the spatiotemporal control and real‐time feedback of macrophage regulatory process is still challenging, leading to off‐targeting effect and delayed efficacy monitoring therefore raising risk of immune overactivation and serious side effects. Herein, a focused ultrasound responsive immunomodulator‐loaded optical nanoplatform (FUSION) is designed to achieve spatiotemporal control and status reporting of macrophage engineering in vivo. Under the stimulation of focused ultrasound (FUS), the immune agonist encapsulated in FUSION can be released to induce selective macrophage M1 phenotype differentiation at tumor site and the near‐infrared mechanoluminescence of FUSION is generated simultaneously to indicate the initiation of immune activation. Meanwhile, the persistent luminescence of FUSION is enhanced due to hydroxyl radical generation in the pro‐inflammatory M1 macrophages, which can report the effectiveness of macrophage regulation. Then, macrophages labeled with FUSION as a living immunotherapeutic agent (FUSION‐M) are utilized for tumor targeting and focused ultrasound activated, immune cell‐based cancer therapy. By combining the on‐demand activation and feedback to form a closed loop, the nanoplatform in this work holds promise in advancing the controllability of macrophage engineering and cancer immunotherapy for precision medicine.

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Direct exposure to mild heat stress stimulates cell viability and heat shock protein expression in primary cultured broiler fibroblasts

Cited by 18 publications

References 44 publications

Exposure to high thermal conditions for a long time induces apoptosis and decreases total RNA concentration in peripheral blood mononuclear cells among Indian Zebu–Jersey crossbreds

Exposure to high thermal conditions for a long time induces apoptosis and decreases total RNA concentration in peripheral blood mononuclear cells among Indian Zebu–Jersey crossbreds

Growth performance of broilers and prevalence of different diseases vary with environmental variation

Closed‐Loop Control of Macrophage Engineering Enabled by Focused‐Ultrasound Responsive Mechanoluminescence Nanoplatform for Precise Cancer Immunotherapy

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