Direct FTIR Assay of Streptomycin in Agar

Meanwell, Richard J.L.; Shama, Gilbert

doi:10.1007/s10529-005-2604-1

Cited by 7 publications

(7 citation statements)

References 7 publications

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“…For instance, Meanwell and Shama utilized FT-IR spectroscopy for isolation of streptomycin hyper-producing mutants on agar. 15 Li et al. and Sayin et al.…”

Section: Introductionmentioning

confidence: 97%

“…For instance, Meanwell and Shama utilized FT-IR spectroscopy for isolation of streptomycin hyperproducing mutants on agar. 15 Li et al and Sayin et al both screened different yeast species by surface-enhanced Raman spectroscopy. 16,17 Helm et al employed FT-IR spectroscopy to detect intracellular components such as granules, capsules, and endospores in intact bacteria.…”

Section: Introductionmentioning

confidence: 99%

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Screening of Astaxanthin-Hyperproducing Haematococcus pluvialis Using Fourier Transform Infrared (FT-IR) and Raman Microspectroscopy

Liu

Huang

2016

Appl Spectrosc

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Haematococcus pluvialis has promising applications owing to its ability to accumulate astaxanthin under stress conditions. In order to acquire higher astaxanthin productivity from H. pluvialis, it is critical not only to develop efficient mutagenesis techniques, but also to establish rapid and effective screening methods which are highly demanded in current research and application practice. In this work, we therefore attempted to develop a new approach to screening the astaxanthin-hyperproducing strains based on spectroscopic tools. Using Fourier transform infrared (FT-IR) and Raman microspectroscopy, we have achieved rapid and quantitative analysis of the algal cells in terms of astaxanthin, β-carotene, proteins, lipids, and carbohydrates. In particular, we have found that the ratio of the IR absorption band at 1740 cm to the band at 1156 cm can be utilized for identifying astaxanthin-hyperproducing strains. This work may therefore open a new avenue for developing high-throughput screening methods necessary for the microbial mutant breeding industry.

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“…For instance, Meanwell and Shama utilized FT-IR spectroscopy for isolation of streptomycin hyper-producing mutants on agar. 15 Li et al. and Sayin et al.…”

Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Screening of Astaxanthin-Hyperproducing Haematococcus pluvialis Using Fourier Transform Infrared (FT-IR) and Raman Microspectroscopy

Liu

Huang

2016

Appl Spectrosc

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“…). Fourier transform infrared and Raman spectroscopy techniques have been applied as promising tools for screening applications in different microbial strains, such as streptomycin hyper‐producing mutants, yeast species and bacteria (Meanwell and Shama ; Sayin et al . ; Liu and Huang ).…”

Section: High‐throughput System For Screening Positive Microbial Strainmentioning

confidence: 99%

“…To our knowledge, microtitre plate wells screening method is usually established when positive microbial strains had obvious phenotype diversity or a bacteriostatic effect . Fourier transform infrared and Raman spectroscopy techniques have been applied as promising tools for screening applications in different microbial strains, such as streptomycin hyper-producing mutants, yeast species and bacteria (Meanwell and Shama 2005;Sayin et al 2009;Liu and Huang 2016). FACS screening technique can count and characterize a large heterogeneous population of cells with fluorescent light using flow cytometry with the rate of up to 10 000 cells per sec (Hyka et al 2013;Yamada et al 2016), and has been used to isolate high-producing microbial or microalgal mutants Terashima et al 2015;Zhang et al 2015).…”

Section: High-throughput System For Screening Positive Microbial Strainmentioning

confidence: 99%

Recent advances of microbial breeding via heavy-ion mutagenesis at IMP

Chen

2017

Lett Appl Microbiol

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Significance and Impact of the Study: There is currently a great interest in developing rapid and diverse microbial mutation tool for strain modification. Heavy-ion mutagenesis has been proved as a powerful technology for microbial breeding due to its broad spectrum of mutation phenotypes with high efficiency. In order to deeply understand heavy-ion mutagenesis technology, this paper briefly reviews recent progress in microbial breeding using heavy-ion mutagenesis at IMP, and also presents the outline of the beam line for microbial breeding in Heavy Ion Research Facility of Lanzhou (HIRFL) as well as new insights into microbial biotechnology via heavy-ion mutagenesis. Thus, this work can provide the guidelines to promote the development of novel microbial biotechnology cross-linking heavy-ion mutagenesis breeding that could make breeding process more efficiently in the future. Abstract Nowadays, the value of heavy-ion mutagenesis has been accepted as a novel powerful mutagen technique to generate new microbial mutants due to its high linear energy transfer and high relative biological effectiveness. This paper briefly reviews recent progress in developing a more efficient mutagenesis technique for microbial breeding using heavy-ion mutagenesis, and also presents the outline of the beam line for microbial breeding in Heavy Ion Research Facility of Lanzhou. Then, new insights into microbial biotechnology via heavy-ion mutagenesis are also further explored. We hope that our concerns will give deep insight into microbial breeding biotechnology via heavy-ion mutagenesis. We also believe that heavy-ion mutagenesis breeding will greatly contribute to the progress of a comprehensive study industrial strain engineering for bioindustry in the future.

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“…To screen the mutants or isolation of the required microalgal strains, traditionally, it is performed by plating the mutated population of microalgal cells onto agar to form colonies, and then the colonies are picked out from the agar followed by cultivation in Erlenmeyer flasks with liquid media for biochemical assay of growth rate, photosynthetic activity, pigment content, gene expression and etc. (Meanwell and Shama 2005;Kamath et al 2008). However, a major drawback of this method is that it is time-and labour-intensive, making it difficult to screen large numbers of microalgae.…”

Section: Introductionmentioning

confidence: 99%