Direct Gas-Liquid Chromatographic Determination of Water-Soluble Vitamin K3 in Feed Premixes

Winkler, Victor W.; Yoder, J.M.

doi:10.1093/jaoac/55.6.1219

Cited by 3 publications

(4 citation statements)

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“…However, most of them have one or more drawback. GLC methods 1,2 are not sufficiently sensitive. Kofler 3 proposed a colorimetric method based on the condensation reaction of 2-methyl-1,4-naphthoquinone with ethyl cyanoacetate and the official colorimetric method of the European Community 4 (EC) for determining MSH includes a timeconsuming colour reaction.…”

mentioning

confidence: 99%

Spectrofluorimetric determination of vitamin K3

Nevado¹,

Laguna²

1998

Analyst

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mentioning

confidence: 99%

Spectrofluorimetric determination of vitamin K3

Nevado¹,

Laguna²

1998

Analyst

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“…Limited methods have been reported for the determination of menadione in vitamin premix or various types of animal feed (Winkler & Yoder 1972; Manz & Maurer 1982; Speek et al. 1984; Laffi et al.…”

Section: Discussionmentioning

confidence: 99%

“…Limited methods have been reported for the determination of menadione in vitamin premix or various types of animal feed (Winkler & Yoder 1972;Manz & Maurer 1982;Speek et al 1984;Laffi et al 1988;Billedeau 1989;Grahl-Madsen & Lie 1997;Roy & Lall 2007). Some of these methods utilize an excessive amount of organic solvent (e.g.…”

Section: Extraction Of K Vitamers From Tissues (Methods A)mentioning

confidence: 99%

Influence of dietary menadione nicotinamide bisulphite (vitamin K3) and phylloquinone (vitamin K1) on Atlantic salmon (Salmo salar L.) tissue levels, determined by high-performance liquid chromatography with fluorescence detection

Graff

Krossøy

Gjerdevik

et al. 2010

Aquaculture Nutrition

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The aim of the present study was to investigate the retention of menadione nicotinamide bisulphite (MNB; vitamin K3) and phylloquinone (vitamin K1) in Atlantic salmon (Salmo salar L.). Another objective was to find a reliable method for determination of menadione in fish feed, and to include and validate more matrices in the methods for phylloquinone and menaquinones (vitamin K2). Duplicate tanks of Atlantic salmon (∼93 g) were fed four levels (0–1000 mg menadione kg−1 feed) of MNB for 9 weeks. The concentration of menadione and phylloquinone in the feed and the concentration of phylloquinone and menaquinone‐4 (MK‐4) in the tissues were determined. The analysed concentration of dietary menadione found in feed indicated a substantial loss of MNB during feed production. This assumption was supported by screening 15 commercial fish feed samples which also revealed menadione concentrations far below the recommended level. MNB fed salmon showed only a minor increase in liver MK‐4 concentration, compared to salmon fed phylloquinone which had a considerably higher level of liver phylloquinone, indicating a higher retention of phylloquinone compared to menadione in Atlantic salmon. Due to highly varying stability and bioavailability of the different vitamin K derivatives, vitamin K supplementation in fish feed needs a revision.

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“…Chromatographic techniques have provided suitable means of determination, e.g., GC with flame ionization detection, [1][2][3] HPLC with UV detection 4 and spectrofluorimetric detection with post-column derivatization based on the reduction of the menadione to a fluorescent derivative (2-methyl-1,4-dihydronaphthoquinone). Post-column reduction with sodium tetrahydroborate (NaBH 4 ) has also been reported.…”

mentioning

confidence: 99%

Spectrofluorimetric Determination of Vitamin K3 by a Solid-phase Zinc Reactor Immobilized in a Flow Injection Assembly

Torró¹,

Mateo²,

Calatayud³

1997

Analyst

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The spectrofluorimetric determination of vitamin K3 (menadione) using a flow injection (FI) assembly provided with a solid-phase reactor with immobilized zinc is described. The naphthohydroquinone was produced by means of two coupled steps in the FI system: hydrolysis of the sodium sulfite derivative of the menadione in a basic medium and reduction of the generated menadione in the zinc reactor in an acidic medium. The fluorescent product was monitored spectrofluorimetrically (lambda ex 325 nm; lambda em 425 nm). The calibration graph was linear over the range 0.1-18 micrograms ml-1 with a reproducibility of 1.6%; the limit of detection was 0.005 microgram ml-1 and the sample throughput was 70 h-1. The influence of foreign compounds was studied and the procedure was applied to the determination of vitamin K3 in three different pharmaceutical formulations.

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Direct Gas-Liquid Chromatographic Determination of Water-Soluble Vitamin K3 in Feed Premixes

Cited by 3 publications

References 0 publications

Spectrofluorimetric determination of vitamin K3

Spectrofluorimetric determination of vitamin K3

Influence of dietary menadione nicotinamide bisulphite (vitamin K3) and phylloquinone (vitamin K1) on Atlantic salmon (Salmo salar L.) tissue levels, determined by high-performance liquid chromatography with fluorescence detection

Spectrofluorimetric Determination of Vitamin K3 by a Solid-phase Zinc Reactor Immobilized in a Flow Injection Assembly

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