Spectrofluorimetric determination of vitamin K3

Nevado, J. J. Berzas; Laguna, Mariano

doi:10.1039/a706114e

Cited by 19 publications

(6 citation statements)

References 16 publications

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“…8 ). Related behavior was reported for MEN, whose hydroquinone form obtained upon chemical reduction also emits in the 420-nm region 35 , 36 . Indeed, reduced MEN displays a π-conjugated system similar to that of the C and D rings of LE–NAC red .…”

Section: Resultssupporting

confidence: 71%

Landomycins as glutathione-depleting agents and natural fluorescent probes for cellular Michael adduct-dependent quinone metabolism

et al. 2021

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Landomycins are angucyclines with promising antineoplastic activity produced by Streptomyces bacteria. The aglycone landomycinone is the distinctive core, while the oligosaccharide chain differs within derivatives. Herein, we report that landomycins spontaneously form Michael adducts with biothiols, including reduced cysteine and glutathione, both cell-free or intracellularly involving the benz[a]anthraquinone moiety of landomycinone. While landomycins generally do not display emissive properties, the respective Michael adducts exerted intense blue fluorescence in a glycosidic chain-dependent manner. This allowed label-free tracking of the short-lived nature of the mono-SH-adduct followed by oxygen-dependent evolution with addition of another SH-group. Accordingly, hypoxia distinctly stabilized the fluorescent mono-adduct. While extracellular adduct formation completely blocked the cytotoxic activity of landomycins, intracellularly it led to massively decreased reduced glutathione levels. Accordingly, landomycin E strongly synergized with glutathione-depleting agents like menadione but exerted reduced activity under hypoxia. Summarizing, landomycins represent natural glutathione-depleting agents and fluorescence probes for intracellular anthraquinone-based angucycline metabolism.

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Section: Resultssupporting

confidence: 71%

Landomycins as glutathione-depleting agents and natural fluorescent probes for cellular Michael adduct-dependent quinone metabolism

et al. 2021

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“…Therefore, the measurements were conducted at room temperature (about 25°C). It was proposed that the reduced products of vitamin K homologues are 1,4-dihydroxy-2-methylnaphthalene derivative (Scheme1), by analogy with the products proposed by reports for reduction of vitamin K 3 by reduction with SnCl 2 [37]. The excitation and emission spectra showed two excitation maxima at 240 and 330 nm and one emission maximum at 430 nm.…”

Section: Optimization Of Fluorescence Derivatization Reactionmentioning

confidence: 93%

“…Therefore, it is necessary to use more efficient and reliable reduction method. Stannous chloride was reported as one of the most efficient derivatization methods for quinones [37]. In this work, we study the effect of different pre-column reduction methods on the fluorescence intensity of using ethanolic solution of SnCl 2 , sodium borohydride, and Zinc powder/acetic acid.…”

Section: Optimization Of Fluorescence Derivatization Reactionmentioning

confidence: 99%

“…Therefore, ethanolic solution of SnCl 2 was selected for the fluorescence induction of the analytes in the present study. SnCl 2 is prone to hydrolysis and decomposition to the basic chloride Sn(OH)Cl plus HCl thus; it has to be prepared in HCl solution [37]. In order to obtain clear and stable solutions without affecting solubility of vitamins K, it was recommended to prepare SnCl 2 solution in a mixture ethanol and HCl (1;1, v/v).…”

Section: Optimization Of Fluorescence Derivatization Reactionmentioning

confidence: 99%

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A novel salting-out assisted extraction coupled with HPLC- fluorescence detection for trace determination of vitamin K homologues in human plasma

Ahmed

Mahmoud

2015

Talanta

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“…The interest in vitamin K 3 has also grown because of its anticancer activity. 1,2 A variety of manual methods have been developed for the determination of menadione using different techniques, such as spectrophotometry, 3,4 polarography, 5 fluorimetry 6,7 and stripping voltammetry. 8,9 Liquid chromatography with different detection systems has also been used.…”

Section: Introductionmentioning

confidence: 99%

Flow injection determination of vitamin K3 by a photoinduced chemiluminescent reaction

Martı́nez-Lozano¹,

Pérez-Ruíz²,

Martı́n³

1999

Analyst

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The determination of vitamin K3 using the coupling between a photochemical reaction and a chemiluminescent reaction in a flow system is described. The method is based on the photooxidation of ethanol sensitized by vitamin K3 to yield hydrogen peroxide, which is monitored through the chemiluminescent reaction with luminol catalysed by hematin. The new approach allows the determination of vitamin K3 in a wide concentration range (1 x 10(-7)-5 x 10(-4) mol l-1) with a throughput of 30 samples h-1. The applicability of the method was demonstrated by the determination of vitamin K3 in pharmaceutical preparations.

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Spectrofluorimetric determination of vitamin K3

Cited by 19 publications

References 16 publications

Landomycins as glutathione-depleting agents and natural fluorescent probes for cellular Michael adduct-dependent quinone metabolism

Landomycins as glutathione-depleting agents and natural fluorescent probes for cellular Michael adduct-dependent quinone metabolism

A novel salting-out assisted extraction coupled with HPLC- fluorescence detection for trace determination of vitamin K homologues in human plasma

Flow injection determination of vitamin K3 by a photoinduced chemiluminescent reaction

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