Direct hydrogen production from dilute-acid pretreated sugarcane bagasse hydrolysate using the newly isolated Thermoanaerobacterium thermosaccharolyticum MJ1

Hu, Binbin; Zhu, Ming‐Jun

doi:10.1186/s12934-017-0692-y

Cited by 28 publications

(9 citation statements)

References 43 publications

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“…4 ). In fact, hydrogen may be produced by Coprothermobacter [ 42 – 44 ], Ruminococcus [ 45 , 46 ], and Thermoanaerobacterium [ 47 , 48 ], and hence any of these organisms may act to syntrophically contribute hydrogen to Methanothermobacter .…”

Section: Discussionmentioning

confidence: 99%

The effect of temperature and retention time on methane production and microbial community composition in staged anaerobic digesters fed with food waste

Gaby

Zamanzadeh

Horn

2017

Biotechnol Biofuels

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BackgroundFood waste is a large bio-resource that may be converted to biogas that can be used for heat and power production, or as transport fuel. We studied the anaerobic digestion of food waste in a staged digestion system consisting of separate acidogenic and methanogenic reactor vessels. Two anaerobic digestion parameters were investigated. First, we tested the effect of 55 vs. 65 °C acidogenic reactor temperature, and second, we examined the effect of reducing the hydraulic retention time (HRT) from 17 to 10 days in the methanogenic reactor. Process parameters including biogas production were monitored, and the microbial community composition was characterized by 16S amplicon sequencing.ResultsNeither organic matter removal nor methane production were significantly different for the 55 and 65 °C systems, despite the higher acetate and butyrate concentrations observed in the 65 °C acidogenic reactor. Ammonium levels in the methanogenic reactors were about 950 mg/L NH4 + when HRT was 17 days but were reduced to 550 mg/L NH4 + at 10 days HRT. Methane production increased from ~ 3600 mL/day to ~ 7800 when the HRT was decreased. Each reactor had unique environmental parameters and a correspondingly unique microbial community. In fact, the distinct values in each reactor for just two parameters, pH and ammonium concentration, recapitulate the separation seen in microbial community composition. The thermophilic and mesophilic digesters were particularly distinct from one another. The 55 °C acidogenic reactor was mainly dominated by Thermoanaerobacterium and Ruminococcus, whereas the 65 °C acidogenic reactor was initially dominated by Thermoanaerobacterium but later was overtaken by Coprothermobacter. The acidogenic reactors were lower in diversity (34–101 observed OTU0.97, 1.3–2.5 Shannon) compared to the methanogenic reactors (472–513 observed OTU0.97, 5.1–5.6 Shannon). The microbial communities in the acidogenic reactors were > 90% Firmicutes, and the Euryarchaeota were higher in relative abundance in the methanogenic reactors.ConclusionsThe digestion systems had similar biogas production and COD removal rates, and hence differences in temperature, NH4 + concentration, and pH in the reactors resulted in distinct but similarly functioning microbial communities over this range of operating parameters. Consequently, one could reduce operational costs by lowering both the hydrolysis temperature from 65 to 55 °C and the HRT from 17 to 10 days.Electronic supplementary materialThe online version of this article (10.1186/s13068-017-0989-4) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

The effect of temperature and retention time on methane production and microbial community composition in staged anaerobic digesters fed with food waste

Gaby

Zamanzadeh

Horn

2017

Biotechnol Biofuels

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“…For example, Lai et al, reported a hydrogen yield of 1.86 mol/mol consumed sugar using T. aotearoense SCUT27/ ∆ldh from SCB hydrolysate, which was slightly higher than the values obtained in this study (1.68–1.79 mol/mol consumed sugar), but the hydrogen productivity (12.43 mmol/L h) of T. aotearoense SCUT27/ ∆ldh was lower than that of SCUT27/ ∆nfnAB [43]. In addition, the newly isolated strain T. thermosaccharolyticum MJ1 showed a hydrogen yield of 2.52 mol/mol consumed sugar from SCB, but the relatively low hydrogen productivity of 6.55 mmol/L h restricted large-scale production of hydrogen by this strain [21]. The results obtained in the present study indicate that the engineered strain SCUT27/ ∆nfnAB was able to directly use dilute acid-pretreated lignocellulose hydrolysates for hydrogen production with high productivity, yield and substrate use ratio, suggesting that it has great potential to produce hydrogen from nonsterilized lignocellulose hydrolysates.…”

Section: Resultsmentioning

confidence: 99%

“…However, the incineration of lignocellulose resources such as corn stalks and wheat straw is the main disposal approach, which causes serious environment issues (global warming and air pollution) and wastes resources (10.75–11.25 billion tons per year) [20]. As cellulose and hemicellulose in lignocellulose can be hydrolyzed to glucose and xylose, lignocellulosic biomass (e.g., sugarcane bagasse [SCB], corn cob, corn stalk, and wheat straw) has the potential to be used for hydrogen production [15, 21, 22].…”

Section: Introductionmentioning

confidence: 99%

Engineered Thermoanaerobacterium aotearoense with nfnAB knockout for improved hydrogen production from lignocellulose hydrolysates

Yang

et al. 2019

Biotechnol Biofuels

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Background As a renewable and clean energy carrier, the production of biohydrogen from low-value feedstock such as lignocellulose has increasingly garnered interest. The NADH-dependent reduced ferredoxin:NADP+ oxidoreductase (NfnAB) complex catalyzes electron transfer between reduced ferredoxin and NAD(P)+, which is critical for production of NAD(P)H-dependent products such as hydrogen and ethanol. In this study, the effects on end-product formation of deletion of nfnAB from Thermoanaerobacterium aotearoense SCUT27 were investigated. Results Compared with the parental strain, the NADH/NAD+ ratio in the ∆nfnAB mutant was increased. The concentration of hydrogen and ethanol produced increased by (41.1 ± 2.37)% (p < 0.01) and (13.24 ± 1.12)% (p < 0.01), respectively, while the lactic acid concentration decreased by (11.88 ± 0.96)% (p < 0.01) when the ∆nfnAB mutant used glucose as sole carbon source. No obvious inhibition effect was observed for either SCUT27 or SCUT27/∆nfnAB when six types of lignocellulose hydrolysate pretreated with dilute acid were used for hydrogen production. Notably, the SCUT27/∆nfnAB mutant produced 190.63–209.31 mmol/L hydrogen, with a yield of 1.66–1.77 mol/mol and productivity of 12.71–13.95 mmol/L h from nonsterilized rice straw and corn cob hydrolysates pretreated with dilute acid. Conclusions The T. aotearoense SCUT27/∆nfnAB mutant showed higher hydrogen yield and productivity compared with those of the parental strain. Hence, we demonstrate that deletion of nfnAB from T. aotearoense SCUT27 is an effective approach to improve hydrogen production by redirecting the electron flux, and SCUT27/∆nfnAB is a promising candidate strain for efficient biohydrogen production from lignocellulosic hydrolysates.

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“…In present study, higher bioethanol yield (0.15 kg/kg TSW) and higher bioethanol concentration (87 g/L), this could decrease the cost of distillation process and is a promising way for bioethanol production. Hu and Zhu (2017) reported that pretreated hydrolysate can be used as a substrate to produce biohydrogen. To improve the bioethanol yield from lignocellulose, the bioethanol fermentation potential of pretreated hydrolysate is deserving of further study.…”

Section: Mass Balance Analysismentioning

confidence: 99%

Enhanced enzymolysis and bioethanol yield from tobacco stem waste based on mild synergistic pretreatment

Gong

Cai³

et al. 2023

Front. Energy Res.

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The use of mild pretreatment conditions can significantly lower the cost of the biorefining process. This study evaluated a mild pretreatment approach for tobacco stem waste using a combination of dilute acid and dilute alkali. By optimizing the pretreatment conditions, we obtained a high reducing sugar concentration at a pretreatment temperature <100°C. Increasing the acid or alkali strength in a single pretreatment step did not always improve the result; instead, the synergistic effect of both pretreatment components was important. To increase the bioethanol titer, a high solid loading, 30%, was used for bioethanol fermentation. Compared with batch fermentation, enzyme feeding with a shorter feeding period (24 h) enhanced the bioethanol production by 4.32%, generating 86.88 g/L bioethanol. The results provide valuable insights into the effects of two-step acid/alkali pretreatment on enzymolysis. The findings suggest that the optimized synergistic pretreatment process is efficient for bioethanol fermentation.

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Direct hydrogen production from dilute-acid pretreated sugarcane bagasse hydrolysate using the newly isolated Thermoanaerobacterium thermosaccharolyticum MJ1

Cited by 28 publications

References 43 publications

The effect of temperature and retention time on methane production and microbial community composition in staged anaerobic digesters fed with food waste

The effect of temperature and retention time on methane production and microbial community composition in staged anaerobic digesters fed with food waste

Engineered Thermoanaerobacterium aotearoense with nfnAB knockout for improved hydrogen production from lignocellulose hydrolysates

Enhanced enzymolysis and bioethanol yield from tobacco stem waste based on mild synergistic pretreatment

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