Direct Identification of On-Bead Peptides Using Surface-Enhanced Raman Spectroscopic Barcoding System for High-Throughput Bioanalysis

Kang, Homan; Jeong, Seong‐Min; Koh, Yul; Geun, Myeong; Yang, Jong In; Kyeong, San; Kim, Jaehi; Kwak, Seon‐Yeong; Chang, Hyejin; Lee, Hyunmi; Jeong, Cheolhwan; Kim, Jong-Ho; Jun, Bong‐Hyun; Kim, Yong-Kweon; Jeong, Dae Hong; Lee, Jun Young

doi:10.1038/srep10144

Cited by 31 publications

(19 citation statements)

References 50 publications

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“…Raman spectroscopy confirmed the selective deposition of the functionalized thiols on finger regions of the gold IDAs ( Supplementary Fig. 6), with the Raman spectra of the thiols matching literature values 42 .…”

Section: Scanning Electron Microscopy (Sem) and Focus Ion Beam (Fib)supporting

confidence: 80%

Dipole-field-assisted charge extraction in metal-perovskite-metal back-contact solar cells

Lin

2017

Proceedings of the 2nd Asia-Pacific Hybrid and Organic Photovoltaics

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Hybrid organic-inorganic halide perovskites are low-cost solution-processable solar cell materials with photovoltaic properties that rival those of crystalline silicon. The perovskite films are typically sandwiched between thin layers of hole and electron transport materials, which efficiently extract photogenerated charges. This affords high-energy conversion efficiencies but results in significant performance and fabrication challenges. Herein we present a simple charge transport layer-free perovskite solar cell, comprising only a perovskite layer with two interdigitated gold back-contacts. Charge extraction is achieved via self-assembled monolayers and their associated dipole fields at the metal-perovskite interface. Photovoltages of~600 mV generated by self-assembled molecular monolayer modified perovskite solar cells are equivalent to the built-in potential generated by individual dipole layers. Efficient charge extraction results in photocurrents of up to 12.1 mA cm −2 under simulated sunlight, despite a large electrode spacing.

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Section: Scanning Electron Microscopy (Sem) and Focus Ion Beam (Fib)supporting

confidence: 80%

Dipole-field-assisted charge extraction in metal-perovskite-metal back-contact solar cells

Lin

2017

Proceedings of the 2nd Asia-Pacific Hybrid and Organic Photovoltaics

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“…SERS is a promising immunodetection technique due to its exceptional sensitivity, specificity, and multiplexing ability with minimal spectral overlap between various reporter molecules. [12][13][14][15][16] The antenna-like behavior of GNSs is attributable to their unique geometry, where their core acts as an antenna and absorbs near-infrared light and their branches behave as emitters to localize the absorbed light at the tips to generate intense electric fields. [17][18][19] We have shown that the near-field electromagnetic radiation generated at the protrusions of GNSs gives rise to a > 10 9 enhancement in SERS signal, resulting in ultrasensitive detection in vitro, in vivo, and in biosensors.…”

mentioning

confidence: 99%

PRADA: Portable Reusable Accurate Diagnostics with nanostar Antennas for multiplexed biomarker screening

Wen

Zarick

et al. 2020

Bioengineering & Transla Med

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Precise monitoring of specific biomarkers in biological fluids with accurate biodiagnostic sensors is critical for early diagnosis of diseases and subsequent treatment planning. In this work, we demonstrated an innovative biodiagnostic sensor, portable reusable accurate diagnostics with nanostar antennas (PRADA), for multiplexed biomarker detection in small volumes (~50 μl) enabled in a microfluidic platform. Here, PRADA simultaneously detected two biomarkers of myocardial infarction, cardiac troponin I (cTnI), which is well accepted for cardiac disorders, and neuropeptide Y (NPY), which controls cardiac sympathetic drive. In PRADA immunoassay, magnetic beads captured the biomarkers in human serum samples, and gold nanostars (GNSs) "antennas" labeled with peptide biorecognition elements and Raman tags detected the biomarkers via surface-enhanced Raman spectroscopy (SERS). The peptide-conjugated GNS-SERS barcodes were leveraged to achieve high sensitivity, with a limit of detection (LOD) of 0.0055 ng/ml of cTnI, and a LOD of 0.12 ng/ml of NPY comparable with commercially available test kits. The innovation of PRADA was also in the regeneration and reuse of the same sensor chip for~14 cycles. We validated PRADA by testing cTnI in 11 de-identified cardiac patient samples of various demographics within a 95% confidence interval and high precision profile. We envision low-cost PRADA will have tremendous translational impact and be amenable to resourcelimited settings for accurate treatment planning in patients.

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“…Nowadays, multi-mode measurement is an inevitable trend in study of cells and biological tissues 32 33 . With the development of SERS technologies, easy-handling, low cost and controllability and reproducibility are important requirements for substrate production 34 35 . The gold plating technique incorporated with ZnO NRs growth can fabricate fine Au nanostructure on microelectrodes with high controllability and low-cost.…”

Section: Resultsmentioning

confidence: 99%

Nanostructured gold microelectrodes for SERS and EIS measurements by incorporating ZnO nanorod growth with electroplating

Zong

Zhu

Guo

2015

Sci Rep

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In this paper, a fine gold nanostructure synthesized on selective planar microelectrodes in micro-chip is realized by using an advanced hybrid fabrication approach incorporating growth of nanorods (NRs) with gold electroplating. By this developed nanostructure, integration of in-situ surface-enhanced Raman spectroscopy (SERS) detection with electrochemical impedance spectroscopy (EIS) measurement for label-free, nondestructive, real-time and rapid monitoring on a single cell has been achieved. Moreover, parameters of Au nanostructures such as size of nanoholes/nanogaps can be controllably adjusted in the fabrication. We have demonstrated a SERS enhancement factor of up to ~2.24 × 106 and double-layer impedance decrease ratio of 90% ~ 95% at low frequency range below 200 kHz by using nanostructured microelectrodes. SERS detection and in-situ EIS measurement of a trapped single cell by using planar microelectrodes are realized to demonstrate the compatibility, multi-functions, high-sensitivity and simplicity of the micro-chip system. This dual function platform integrating SERS and EIS is of great significance in biological, biochemical and biomedical applications.

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Direct Identification of On-Bead Peptides Using Surface-Enhanced Raman Spectroscopic Barcoding System for High-Throughput Bioanalysis

Cited by 31 publications

References 50 publications

Dipole-field-assisted charge extraction in metal-perovskite-metal back-contact solar cells

Dipole-field-assisted charge extraction in metal-perovskite-metal back-contact solar cells

PRADA: Portable Reusable Accurate Diagnostics with nanostar Antennas for multiplexed biomarker screening

Nanostructured gold microelectrodes for SERS and EIS measurements by incorporating ZnO nanorod growth with electroplating

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