Direct immobilization of fluorescent dyes onto ferrite nanoparticles during their synthesis from aqueous solution

Abstract

FITC-avidin, a fluorescent substance, was successfully immobilized onto the surfaces of nanoparticles of ferrite of a mixture between Fe3O4 and γ-Fe2O3, during the synthesis of the nanoparticles an aqueous solution from containing Fe2+, Fe3+, and FITC-avidin. The particles were spherical in shape and ∼10 nm in size. The process was performed in open air, on near neutral conditions of pH 7–9, and at ∼4 °C. Utilizing the fluorescent magnetic particles, we successfully observed magnetic patterns (track pitch 187.5 μm) written on a floppy disk.

Preparation and medical application of magnetic beads conjugated with bioactive molecules

“…1) 4 1C and under nearly neutral conditions of pH ¼ 7-9, which is compatible with most functional biomolecules [2,3].…”

Magnetic luminescent nanoparticles as internal calibration for an immunoassay for ricin

“…Several groups have reported successful synthesis and characterization of particles that possess both fluorescent and magnetic properties (1)(2)(3)(4)(5)(6)(7). In most of the cases, the synthesis of particles with magnetic and fluorescent properties is complicated and expensive.…”

Microfluidic synthesis of fluorescent magnetonanoparticles and characterization

“…Most fluorescent magnetic nanocomposites are core-shell nanostructures coated with a polymer functionalized with a fluorescent dye. The polymer coating is advantageous because it makes the solid MNPs soft, prevents oxidation, and can improve biocompatibility [13]. Recent developments to improve biocompatibility of the fluorescent MNPs have used chitosan of a biocompatible compound modified with a fluorophore to coat the MNPs [14].…”