Direct inhibition of phosphate transport by immune signaling in Arabidopsis

Dindas, Julian; DeFalco, Thomas A.; Yu, Gang; Zhang, Lu; David, Pascale; Bjornson, Marta; Thibaud, Marie-Christine; Custódio, Valéria; Castrillo, Gabriel; Nussaume, Laurent; Macho, Alberto P.; Zipfel, Cyril

doi:10.1016/j.cub.2021.11.063

Cited by 34 publications

(24 citation statements)

References 59 publications

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“…Recent evidence suggests a direct integration of nutrient homeostasis and PTI in plants. Perception of flg22 induces PHT1.4 phosphorylation to inhibit phosphate (Pi) uptake and promote root immunity (36).…”

Section: Discussionmentioning

confidence: 99%

CEP signaling coordinates plant immunity with nitrogen status

Rzemieniewski

Leicher

Lee

et al. 2022

Preprint

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Little is known about plant genetic and biochemical components that coordinate immune responses with growth and environmental cues. C-TERMINALLY ENCODED PEPTIDEs (CEPs) control plant development and nitrogen demand signaling. Here, we identified CEP4 as an immune-modulatory peptide (phytocytokine) in Arabidopsis thaliana. CEP4 and related CEPs are important regulators of resistance to plant pathogenic bacteria and are perceived by the tissue-specific receptor kinases CEP RECEPTOR 1 (CEPR1), CEPR2 and the phylogenetically related RECEPTOR-LIKE KINASE 7 (RLK7). CEP4 promotes flagellin-triggered responses and we provide evidence that CEPs modulate cell surface immunity upon N limitation. We propose that CEPs integrate biotic and abiotic stress-associated signals to safeguard plant health.

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Section: Discussionmentioning

confidence: 99%

CEP signaling coordinates plant immunity with nitrogen status

Rzemieniewski

Leicher

Lee

et al. 2022

Preprint

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“…Very recently, Dindas et al. (2022) described a negative regulation of Pi transport by immune signaling in Arabidopsis. In citrus plants, Pi content was associated to symptomology in Huanglongbing (HLB) disease, where Pi deficiency favors development of HLB symptoms (Zhao et al., 2013).…”

Section: Discussionmentioning

confidence: 99%

“…The PSR system also appears to control root colonization by the endophytic fungus Colletotrichum tofieldiae in Arabidopsis (Frerigmann et al, 2021;Hiruma et al, 2016). Very recently, Dindas et al (2022) described a negative regulation of Pi transport by immune signaling Arabidopsis. In citrus plants, Pi content was associated to symptomology in Huanglongbing (HLB) disease, where Pi deficiency favors development of HLB symptoms (Zhao et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

Phosphate‐induced resistance to pathogen infection in Arabidopsis

et al. 2022

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In nature, plants are concurrently exposed to a number of abiotic and biotic stresses. Our understanding of convergence points between responses to combined biotic/abiotic stress pathways remains, however, rudimentary. Here we show that MIR399 overexpression, loss-of-function of PHOSPHATE2 (PHO2), or treatment with high phosphate (Pi) levels is accompanied by an increase in Pi content and accumulation of reactive oxygen species (ROS) in Arabidopsis thaliana. High Pi plants (e.g., miR399 overexpressors, pho2 mutants, and plants grown under high Pi supply) exhibited resistance to infection by necrotrophic and hemibiotrophic fungal pathogens. In the absence of pathogen infection, the expression levels of genes in the salicylic acid (SA)-and jasmonic acid (JA)-dependent signaling pathways were higher in high Pi plants compared to wild-type plants grown under control conditions, which is consistent with increased levels of SA and JA in non-infected high Pi plants. During infection, an opposite regulation in the two branches of the JA pathway (ERF1/PDF1.2 and MYC2/VSP2) occurs in high Pi plants. Thus, while pathogen infection induces PDF1.2 expression in miR399 OE and pho2 plants, VSP2 expression is downregulated by pathogen infection in these plants. This study supports the notion that Pi accumulation promotes resistance to infection by fungal pathogens in Arabidopsis, while providing a basis to better understand interactions between Pi signaling and hormonal signaling pathways for modulation of plant immune responses.

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“…MAMP exposure induces K + and Mn 2+ loss and accumulation of P 3– S 2– , Zn 2+ , and Fe 3+ in Arabidopsis seedlings within minutes of flg22 application though this shift is transient since Fe 3+ accumulation returns to control levels by 6 h ( Brauer et al, 2016 ; Nobori et al, 2018 ). Indeed, suppression of phosphate transport through the PHT1;4 uptake transporter in roots is regulated downstream of MAMP recognition by BIK1 and PBL1 receptor-like cytosolic kinases and influences infection by Ralstonia solanacearum ( Dindas et al, 2022 ). On the pathogen side, P. syringae DC3000 sulfur starvation responses are induced when plants are pre-treated with flg22 indicating that bacteria may sense the nutritional shift in plants during immune activation ( Lovelace et al, 2018 ).…”

Section: Discussionmentioning

confidence: 99%

Back From the Dead: The Atypical Kinase Activity of a Pseudokinase Regulator of Cation Fluxes During Inducible Immunity

Brauer

Ahsan²,

Popescu³

et al. 2022

Front. Plant Sci.

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Pseudokinases are thought to lack phosphotransfer activity due to altered canonical catalytic residues within their kinase domain. However, a subset of pseudokinases maintain activity through atypical phosphotransfer mechanisms. The Arabidopsis ILK1 is a pseudokinase from the Raf-like MAP3K family and is the only known plant pseudokinase with confirmed protein kinase activity. ILK1 activity promotes disease resistance and molecular pattern-induced root growth inhibition through its stabilization of the HAK5 potassium transporter with the calmodulin-like protein CML9. ILK1 also has a kinase-independent function in salt stress suggesting that it interacts with additional proteins. We determined that members of the ILK subfamily are the sole pseudokinases within the Raf-like MAP3K family and identified 179 novel putative ILK1 protein interactors. We also identified 70 novel peptide targets for ILK1, the majority of which were phosphorylated in the presence of Mn2+ instead of Mg2+ in line with modifications in ILK1’s DFG cofactor binding domain. Overall, the ILK1-targeted or interacting proteins included diverse protein types including transporters (HAK5, STP1), protein kinases (MEKK1, MEKK3), and a cytokinin receptor (AHK2). The expression of 31 genes encoding putative ILK1-interacting or phosphorylated proteins, including AHK2, were altered in the root and shoot in response to molecular patterns suggesting a role for these genes in immunity. We describe a potential role for ILK1 interactors in the context of cation-dependent immune signaling, highlighting the importance of K+ in MAMP responses. This work further supports the notion that ILK1 is an atypical kinase with an unusual cofactor dependence that may interact with multiple proteins in the cell.

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Direct inhibition of phosphate transport by immune signaling in Arabidopsis

Cited by 34 publications

References 59 publications

CEP signaling coordinates plant immunity with nitrogen status

CEP signaling coordinates plant immunity with nitrogen status

Phosphate‐induced resistance to pathogen infection in Arabidopsis

Back From the Dead: The Atypical Kinase Activity of a Pseudokinase Regulator of Cation Fluxes During Inducible Immunity

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