Direct laser writing by two-photon polymerization as a tool for developing microenvironments for evaluation of bacterial growth

Otuka, Adriano J. G.; Corrêa, Daniel S.; Fontana, Carla Raquel; Mendonça, Cleber Renato

doi:10.1016/j.msec.2013.11.005

Cited by 16 publications

(13 citation statements)

References 25 publications

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“…Rastering the laser focus throughout the photoresist in three dimensions then enables the creation of polymer structures with virtually any geometry. [7-9] This architectural versatility renders these 3D polymer structures useful for many technological applications, including drug delivery, [10-12] tissue engineering, [13-15] micro/nano-optics [16-17] and photonics. [18-19] …”

Section: Introductionmentioning

confidence: 99%

“…Rastering the laser focus throughout the photoresist in three dimensions then enables the creation of polymer structures with virtually any geometry. [7][8][9] This architectural versatility renders these 3D polymer structures useful for many technological applications, including drug delivery, [10][11][12] tissue engineering, [13][14][15] micro-/nano-optics, [16,17] and photonics. [18,19] To further expand the application of these 3D structures, it is not only important to precisely engineer the structure and architecture of the material, it is also necessary to have control over the chemical functionality on the surface and/or in the volume of these 3D structures.…”

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confidence: 99%

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Functionalized 3D Architected Materials via Thiol‐Michael Addition and Two‐Photon Lithography

et al. 2017

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Graphical abstract A facile method of fabricating functionalized 3D architected materials is achieved by using functionalized acrylates synthesized via thiol-Michael addition, which are then polymerized using two-photon lithography. A wide variety of functional groups can be attached, from Boc-protected amines to fluoroalkanes. Modification of surface wetting properties and conjugation with fluorescent tags are demonstrated to highlight the potential applications of this technique.

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Section: Introductionmentioning

confidence: 99%

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confidence: 99%

Functionalized 3D Architected Materials via Thiol‐Michael Addition and Two‐Photon Lithography

et al. 2017

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“…In this particular case, 3D structures have been used to control the bacterial growth (E. coli) and to investigate their trapping and migration. 46 Cell force measurement 2pp fabricated scaffolds are able to physically stimulate the cells, but, conversely, also cells can exert forces and elastically deform these structures: in particular, neurites and cardiomyocyte forces have been measured by exploiting 2pp structure deformability. 34,43 The biophysical measurement of these forces is particularly important not only for the investigation of the cell-cell mechanical communication systems, but also for the analysis of their consequence on the mechanical properties and functions of the deriving tissues.…”

Section: Pp Structures For Biological Investigations Cell/substrate mentioning

confidence: 99%

Biomimicry at the nanoscale: current research and perspectives of two-photon polymerization

et al. 2015

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Living systems such as cells and tissues are extremely sensitive to their surrounding physico-chemical microenvironment. In the field of regenerative medicine and tissue engineering, the maintenance of culture conditions suitable for the formation of proliferation niches, for the self-renewal maintenance of stem cells, or for the promotion of a particular differentiation fate is an important issue that has been addressed using different strategies. A number of investigations suggests that a particular cell behavior can be in vitro resembled by mimicking the corresponding in vivo conditions. In this context, several biomimetic environments have been designed in order to control cell phenotypes and functions. In this review, we will analyze the most recent examples of the control of the in vitro physical micro/nano-environment by exploiting an innovative technique of high resolution 3D photolithography, the two-photon polymerization (2pp). The biomedical applications of this versatile and disruptive computer assisted design/manufacturing technology are very wide, and range from the fabrication of biomimetic and nanostructured scaffolds for tissue engineering and regenerative medicine, to the microfabrication of biomedical devices, like ossicular replacement prosthesis and microneedles.

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“…Otuka et al [47] used 2PP to fabricate microenvironment for in situ monitoring of cell growth and movement. They fabricated a microenvironment that was doped at specific site with ciprofloxacin (an antibiotic that is used in the treatment of diseases caused by E. coli) and that included microfences that can trap bacteria.…”

Section: Two-photon Polymerization Technology For Tissue Engi-mentioning

confidence: 99%

3D Nanoprinting Technologies for Tissue Engineering Applications

Lee

2015

Journal of Nanomaterials

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Tissue engineering recovers an original function of tissue by replacing the damaged part with a new tissue or organ regenerated using various engineering technologies. This technology uses a scaffold to support three-dimensional (3D) tissue formation. Conventional scaffold fabrication methods do not control the architecture, pore shape, porosity, or interconnectivity of the scaffold, so it has limited ability to stimulate cell growth and to generate new tissue. 3D printing technologies may overcome these disadvantages of traditional fabrication methods. These technologies use computers to assist in design and fabrication, so the 3D scaffolds can be fabricated as designed and standardized. Particularly, because nanofabrication technology based on two-photon absorption (2PA) and on controlled electrospinning can generate structures with submicron resolution, these methods have been evaluated in various areas of tissue engineering. Recent combinations of 3D nanoprinting technologies with methods from molecular biology and cell dynamics have suggested new possibilities for improved tissue regeneration. If the interaction between cells and scaffold system with biomolecules can be understood and controlled and if an optimal 3D environment for tissue regeneration can be realized, 3D nanoprinting will become an important tool in tissue engineering.

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Direct laser writing by two-photon polymerization as a tool for developing microenvironments for evaluation of bacterial growth

Cited by 16 publications

References 25 publications

Functionalized 3D Architected Materials via Thiol‐Michael Addition and Two‐Photon Lithography

Functionalized 3D Architected Materials via Thiol‐Michael Addition and Two‐Photon Lithography

Biomimicry at the nanoscale: current research and perspectives of two-photon polymerization

3D Nanoprinting Technologies for Tissue Engineering Applications

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