Direct mapping of adeno-associated virus capsid proteins B and C: a possible ACG initiation codon.

Abstract

The three major capsid proteins of adeno-

uORFs with unusual translational start codons autoregulate expression of eukaryotic ornithine decarboxylase homologs

“…Initiation of eukaryotic translation on codons differing at a single position from AUG is inefficient and rare (25). The nucleotides surrounding the AUU codon in antizyme inhibitor uCC comply with the standard context for efficient initiation (15) although not to the same extent as those flanking some previously identified non-AUG-initiating codons (26).…”

Parvovirus Gene Regulation

“…The seeming paradox is apparently resolved in two ways. VP-2 has been reported to initiate translation at an ACG upstream from the first potential AUG (Becerra et al, 1985). VP-1 translation may require a second splice of the p40 transcript, either in addition to or in place of that shown in Fig.…”

Yeast cells may use AUC or AAG as initiation codon for protein synthesis

“…BECERRA et al (2) speculate that use of non-AUG as initiation codon for translation of the AAV-B protein could regulate the relative synthesis of proteins B and C in vivo if these proteins are translated from the same mRNA. Assuming the initiation signal for AAV-B protein (initiation codon ACG) is weak compared to the signal for AAV-C protein (initiation codon AUG), then it would be possible for the cell to obtain a fixed ratio of B protein/C protein.…”

“…BECERRA et at. (2) have shown that the methionyl-tRNA dependent initiation ofadeno-associated virus (AAV) B protein starts from an ACG codon, and ANDERSON et al (1) have shown that an ACG codon (replacing the AUG used in vivo to initiate the synthesis of Abbreviations: ADHI (PR) = alcohol dehydrogenase I (promoter); bp = base pair(s).…”