The film thickness of a hanging soap bubble has been studied along its gravitational orientation after its birth and before its bursting using large lateral shearing displacement interferometry, with a theoretical error of less than 0.325λ. The results show that the spatial distribution of the film thickness could be approximated with an exponential model in all captured frames, especially in the lower half of the soap bubble. Before its bursting, a special zone, where the water layer has drained out while the surfactant solution layer remains, will occur at the top of the soap bubble and gradually expand toward the bottom. Moreover, the simulated fringe patterns based on the computed values match well with the experimentally observed ones.