We report direct numerical simulations (DNSs) of open-channel flow with a step change from three-dimensional sinusoidal rough surface to smooth surface. We investigate the persistence of non-equilibrium behaviour beyond this step change (i.e. departures from the equilibrium smooth openchannel flow) and how this depends on 1) roughness virtual origin /h? (scaled by the channel height h), 2) roughness size k/h?, 3) roughness shape? and 4) Reynolds number Re τ ? To study (1), the roughness origin was placed aligned with, below (step-up) and above (step-down) the smooth patch. To study (2), the equivalent sand-grain roughness of the aligned case was decreased from k + s 160 to 106. To study (3) and ( 4) the step-down case at Re τ 395 was compared with a backward-facing step case at Re τ 527, and DNS of square rib rough-to-smooth case at Re τ 1160 (Ismail et al., J. Fluid Mech., vol. 843, 2018, pp. 419-449). Results showed that /h affects the departure from equilibrium by a large extent, while k/h, roughness shape and Re τ have a marginal influence. The departure from equilibrium was found to be related to the near-wall amplification of Reynolds shear stress, which in turn depends on /h, i.e. higher /h leads to higher amplification.