Direct Patterning of Protein‐ and Cell‐Resistant Polymeric Monolayers and Microstructures

Khademhosseini, Ali; Jon, Sangyong; Suh, Kahp Y.; Tran, Thanh-Nga T.; Eng, George; Yeh, Judy; Seong, Jiehyun; Langer, Robert

doi:10.1002/adma.200305433

Cited by 125 publications

(118 citation statements)

References 27 publications

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“…[15][16][17][18] The use of sophisticated threedimensional (3D) hydrogel structures would enable important advances in drug delivery, tissue engineering, and favorable material-device interaction. [19][20][21][22][23] For example, 2D cell cultures do not properly imitate the microambiance of threedimensionally-organized native tissue.…”

mentioning

confidence: 99%

Three-Dimensionally-Patterned Submicrometer-Scale Hydrogel/Air Networks That Offer a New Platform for Biomedical Applications

et al. 2008

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Phase mask interference lithography was employed to fabricate three-dimensional (3D) hydrogel structures with high surface area on neural prosthetic devices. A random terpolymer of poly(hydroxyethyl methacrylate-ran-methyl methacrylate-ran-methacrylic acid) was synthesized and used as a negative-tone photoresist to generate bicontinuous 3D hydrogel structures at the submicrometer scale. We demonstrated that the fully open 3D hydrogel/air networks can be used as a pH-responsive polymeric drug-release system for the delivery of neurotrophins to enhance the performance of neural prosthetic devices. Additionally an open hydrogel structure will provide direct access of neuronal growth to the device for improved electrical coupling.

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mentioning

confidence: 99%

Three-Dimensionally-Patterned Submicrometer-Scale Hydrogel/Air Networks That Offer a New Platform for Biomedical Applications

et al. 2008

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“…In comparison with CP, the technique generates features varying in height with precise control over cell migration and interconnection of cell arrays. 6 If water is used as a solvent, the wetting environment is much different from previous studies of micromolding of hydrophobic polymers such as polystyrene involving organic solvents. 9 For example, instead of filling into the mold, a water solution under the void space recedes downwards ͑"capillary depression"͒ until the substrate surface becomes exposed ͑"dewetting"͒ due to an obtuse contact angle of water on PDMS mold ͑ϳ105°͒ ͑Ref.…”

Section: Introductionmentioning

confidence: 85%

“…2 and 3͒ or polyethylene glycol ͑PEG͒, [4][5][6][7][8] which is a form of soft lithography, has been introduced as an alternative to microcontact printing ͑ CP͒ for the development of protein and cell arrays. In previous work, we reported a direct molding method using polydimethylsiloxane ͑PDMS͒ mold, which involves the placement of a patterned PDMS mold on top of a dropdispensed or spin-coated HA/PEG solution typically dissolved in water or ethanol, leaving behind a polymer replica after solvent evaporation followed by mold removal.…”

Section: Introductionmentioning

confidence: 99%

On the thickness uniformity of micropatterns of hyaluronic acid in a soft lithographic molding method

Jeong¹,

Suh²

2005

Journal of Applied Physics

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A soft lithographic molding is a simple and yet robust method for fabricating well-defined microstructures of a hydrophilic biopolymer such as polyethylene glycol and polysaccharide over a large area. The method consists of three steps: placing a polydimethylsiloxane mold with a bas-relief pattern onto a drop-dispensed polymer solution typically dissolved in water, letting the mold and the solution undisturbed in contact until solvent evaporates completely, and leaving behind a polymer replica after mold removal. In such a molding process, water can only evaporate from the edges of the mold due to impermeable nature of polydimethylsiloxane to water, resulting in a nonuniform distribution of film thickness or pattern height. Here we examine systematically how the evaporation rate affects the thickness distribution of the resulting microstructures by evaporating the solution of hyaluronic acid in various conditions. To compare with a theory, we also present a simple theoretical model based on one-dimensional conservation equation for a liquid film, which is in good agreement with the experimental data.

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“…The extracellular microenvironment have a significant influence on cancer cell behaviors including their migration, adhesion, differentiation, proliferation, and morphogenesis (Khademhosseini et al 2003;Kim et al 2012;Shen et al 2008). Adhesive, soluble, and mechanical cues can be used for spatially organizing cells and achieving the desired tissue function.…”

Section: Introductionmentioning

confidence: 99%

Selective pattern of cancer cell accumulation and growth using UV modulating printing of hydrogels

Wei

et al. 2015

Biomed Microdevices

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Fabrication of extracellular microenvironment for cancer cell growth in vitro is an indispensable technique to precisely control the cell spatial arrangement and proliferation for cell-behavior research. Current micropatterning methods usually require relatively complicated operations, which makes it difficult to investigate the effects of different cell growth patterns. This manuscript proposes a DMD-based projection technique to quickly pattern a poly(ethylene) glycol diacrylate (PEGDA)-based hydrogel on a common glass substrate. Using this method, we can effectively control the growth patterns of cells. Compared with these traditional methods which employ digital dynamic mask, polymerization of PEGDA solution can be used to create arbitrary shaped microstructures with high efficiency, flexibility and repeatability. The duration of UV exposure is less than 10 s through controlling the projected illumination pattern. The ability of patterned PEGDA-coated film to hinder cell adhesion makes it possible to control area over which cells attach. In our experiments, we take advantage of the blank area to pattern cells, which allows cells to grow in various pre-designed shapes and sizes. And the patterning cells have a high viability after culturing for several days. Interestingly, we found that the restricted space could stiffen and strengthen the cells. These results indicate that cells and extracellular microenvironment can influence each other.

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Direct Patterning of Protein‐ and Cell‐Resistant Polymeric Monolayers and Microstructures

Cited by 125 publications

References 27 publications

Three-Dimensionally-Patterned Submicrometer-Scale Hydrogel/Air Networks That Offer a New Platform for Biomedical Applications

Three-Dimensionally-Patterned Submicrometer-Scale Hydrogel/Air Networks That Offer a New Platform for Biomedical Applications

On the thickness uniformity of micropatterns of hyaluronic acid in a soft lithographic molding method

Selective pattern of cancer cell accumulation and growth using UV modulating printing of hydrogels

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