Direct, preparative enantioselective chromatography of propranolol hydrochloride and thioridazine hydrochloride using carbon dioxide-based mobile phases

Geiser, Fiona; Schultz, Martin; Betz, L. D.; Shaimi, Mohamed; Lee, James; Champion, William L.

doi:10.1016/s0021-9673(99)01117-6

Cited by 41 publications

(15 citation statements)

References 9 publications

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“…12 Enantioselective separation of each of the target analytes was first attempted in the absence of any additive to establish analyte retention and observe chromatographic peak shape. Additives were then incorporated into the methanol modifier at various levels and the effects on retention, enantioselectivity, and resolution were monitored.…”

Section: Resultsmentioning

confidence: 99%

Additive concentration effects on enantioselective separations in supercritical fluid chromatography

Phinney

Sander

2003

Chirality

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Polar additive concentration effects in supercritical fluid chromatography were studied on chiral stationary phases having either a macrocyclic glycopeptide or a derivatized polysaccharide as the chiral selector. Two basic additives, isopropylamine and triethylamine, were incorporated into the methanol modifier at various concentrations and the effects on retention, selectivity, and resolution were monitored. Many of the analytes failed to elute from the macrocyclic glycopeptide stationary phase in the absence of an additive and the most noticeable effect of increasing additive concentration was a significant decrease in retention. On the derivatized polysaccharide stationary phase the additives had little effect on retention, but they did foster significant improvements in peak shape and resolution.

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Section: Resultsmentioning

confidence: 99%

Additive concentration effects on enantioselective separations in supercritical fluid chromatography

Phinney

Sander

2003

Chirality

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“…Among the methods, voltammetry [8][9][10] and conventional high performance liquid chromatography 11,12 are most often used. Supercritical fluid chromatography 13 and HPLC combined with other techniques [14][15][16][17][18] were reported for determination of TRH and its main metabolites or derivatives. Chemiluminescence 19 , electrophoresis 20 , spectrofluorimetric 21,22 and spectrophotometry [23][24][25][26] have been also reported for the determination of thioridazine hydrochloride.…”

Section: Chemicals and Materialsmentioning

confidence: 99%

Two Selective Spectrophotometry Methods for the Determination of Thioridazine Hydrochloride in Tablets and in Biological Fluids

El‐Didamony

Moustafa

2012

J. Chil. Chem. Soc.

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Two simple, rapid and selective spectrophotometric methods have been developed for the analysis of the psychoactive drug thioridazine HCl (TRH) in tablets and in biological fluids. The first method is based on the oxidation of TRH by a known excess of N-bromosuccinimide (NBS), followed by the determination of unreacted oxidants by measuring the decrease in absorbance of two different dyes; amaranth (AM) and methylene blue (MB) at a suitable l max 520 and 660 nm, respectively. Beer-Lambert plots showed good correlation in the concentration ranges of 0.8-4.8 and 0.8-5.6 µg/ml for AM and MB methods, respectively. The second method is based upon the formation of an ion-pair complexes (1: 1) with the acidic sulphonphthalein dyes bromophenol blue (BPB) and bromothymol blue (BTB). The formed complexes were extracted into methylene chloride and their absorbance was measured at 405 and 408 nm for BPB and BTB, respectively. Beer's law was obeyed with a good correlation coefficient (r = 0.9995-0.9989) in the concentration ranges of 4 -24 µg/ml for both BPB and BTB methods, respectively. All measurements of the two procedures are carried out in an acidic medium at room temperature. Optimizations of the different experimental conditions are described for both methods. The two methods have been successfully applied to the determination of thioridazine HCl in pharmaceutical, serum and urine samples and average recoveries are in the range of 98.12 -102.55%. Analytical results obtained with this novel method are satisfactory.

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“…column were 40 mg and 32 mg for propranolol HCl and thioridazine HCl, respectively, and the enantiomeric excess was better than 99.2% and 98.2%, respectively. 127 The most often used preparative chromatographic enantioseparation is simulated moving bed technology applied for such drugs as tramadol (productivity, with a feed concentration of 20 g/l, at a feed flow- 128 guaifenesin (on Chiralcel OD, productivity, 80.6 g/day/kg chiral stationary phase) 129,130 and others (formoterol, aminoglutethimide) 129,131 or for some pharmaceutical intermediates (ester of quinoline mevalonic acid). 132,133 A similar method, called closed-loop steady state recycling, was also used for that purpose.…”

Section: à125mentioning

confidence: 99%

Recent applications of stereoselective chromatography

2002

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Direct, preparative enantioselective chromatography of propranolol hydrochloride and thioridazine hydrochloride using carbon dioxide-based mobile phases

Cited by 41 publications

References 9 publications

Additive concentration effects on enantioselective separations in supercritical fluid chromatography

Additive concentration effects on enantioselective separations in supercritical fluid chromatography

Two Selective Spectrophotometry Methods for the Determination of Thioridazine Hydrochloride in Tablets and in Biological Fluids

Recent applications of stereoselective chromatography

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